In-silico analysis was performed to analyse the promoters of four MIOX genes in Arabidosis thaliana. The analyses revealed that several regulatory elements were present in upstream promoter region (1200 bp from start codon) of Arabidopsis MIOX (AtMIOX) genes. Some of regulatory elements were common while the number of elements differed in AtMIOX promoter sequences. The highest expression of AtMIOX4 was noticed in flower and pistil, whereas AtMIOX1 and AtMIOX5 were highly expressed in endosperm of seed. MYB binding site (MBS) involved in drought stress were present in promoter sequence of AtMIOX2 and AtMIOX4. Expression pattern of MIOX genes positively correlated with the similar number of cis-regulatory elements and motifs presented in the promoter regions. The presence of specific cis-regulatory elements and motifs indicate that the response of MIOX is highly controlled by abiotic (heat and drought) and biotic (fungi and bacteria) stresses. The information generated by this study could pave the way for functional and crop improvement studies in economically important crop plants.