Aim: This study aims to investigate the effects of four weeks cardio-Pilates exercise program on physical characteristics in females. Material and methods: The total 40 female participants were tested before and after four weeks regular exercise of 3 × 1 hr. sessions/week. Body height and weight, waist and hip circumferences, body fat percent and weight were measured. To test the differences, a paired t-test was used. Results: This study showed that cardio-Pilates exercise program significantly decreased body fat percent, body fat weight, waist and hip circumferences. Cohen's d calculations showed that four weeks exercise program had a significant moderate effect on body fat percent, body fat weight, waist and hip circumferences in females. Conclusion: Four weeks exercise program seems to be effective in decreasing the body fat and circumference values in females. It was concluded that the magnitude of training effect depends on the duration and intensity of the exercise program.