Ethanol is a polar organic solvent, and frequently used as a fuel in automobile industries, principally as an additive with gasoline due to its higher octane rating. It is generally produced from biomass such as corn, sugar and some other agriculture products. In the present study, impact of biofield treatment on ethanol was evaluated with respect to its atomic and thermal properties. The ethanol sample was divided into two parts i.e., control and treatment. Control part was remained untreated. Treatment part was subjected to Mr. Trivedi’s biofield treatment. Control and treated samples were characterized using Gas chromatography-mass Spectrometry (GC-MS), Differential scanning calorimetry (DSC), and High performance liquid chromatography (HPLC). GC-MS data revealed that isotopic abundance of 13C i.e., δ13C of treated ethanol was significantly changed from -199‰ upto 155‰ as compared to control. The DSC data exhibited that the latent heat of vaporization of treated ethanol was increased by 94.24% as compared to control, while no significant change was found in boiling point. Besides, HPLC data showed that retention time was 2.65 minutes in control, was increased to 2.76 minutes in treated ethanol sample. Thus, overall data suggest that biofield treatment has altered the atomic and thermal properties of ethanol.