1 online resource (xix, 668 pages) :

In recent years, large-scale advances in technology have led to greater understanding of the world on a much tinier scale: the biomolecular level. In Micro and Nano Technologies in Bioanalysis: Methods and Protocols, expert researchers from across the globe explore the technology which makes this analysis possible, investigating the worlds of microfluidics and nanotechnologies, and examining physical science techniques for the separation, detection, manipulation, and analysis of biomolecules. This volume contains innovative protocols on the application of microfluidics and the utilization of physical science-related technologies that will prove to be invaluable in the field of molecular biology. Chapters contain cutting edge applications of emerging nanotechnologies, including quantum dots and molecular fluorescence for the imaging and tracking of biomolecules. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and groundbreaking, Micro and Nano Technologies in Bioanalysis: Methods and Protocols is a necessary tool for cellular biologists, biochemists, microbiologists, geneticists and medical researchers alike

Includes bibliographical references and index

HPLC-chip/MS technology in proteomic profiling / M. Vollmer and T. Goor -- Nanofluidic channel fabrication and manipulation of DNA molecules / K.-G. Wang and H. Niu -- A single-molecule barcoding system using nanoslits for DNA analysis / K. Jo, T.M. Schramm and D.C. Schwartz -- Microfluidic devices with photodefinable pseudo-valves for protein separation / Z.H. Fan -- Microfluidic chips designed for measuring biomolecules through a microbead-based quantum dot fluorescence assay / K.-S. Yun [and others] -- DNA focusing using microfabricated electrode arrays / F.A. Shaikh and V.M. Ugaz -- Solid-state nanopore for detecting individual biopolymers / J. Li and J.A. Golovchenko -- Inserting and manipulating DNA in a nanopore with optical tweezers / U.F. Keyser [and others] -- Forming an [alpha]-hemolysin nanopore for single-molecule analysis / N.N. Jetha, M. Wiggin and A. Marziali -- Nanopore force spectroscopy on DNA duplexes / N.N. Jetha, M. Wiggin and A. Marziali -- Quantitative chemical analysis of single cells / M.L. Heien and A.G. Ewing -- Trapping and detection of single molecules in water / M. Willander [and others] -- ZnO nanorods as an intracellular sensor for ph measurements / M. Willander and S. Al-Hilli -- Analysis of biomolecules using surface plasmons / M. Willander and S. Al-Hilli -- Use of residual dipolar couplings in structural analysis of protein-ligand complexes by solution NMR spectroscopy / N.U. Jain -- Raman-assisted x-ray crystallography for the analysis of biomolecules / D. Bourgeois [and others] -- Methods and software for diffuse x-ray scattering from protein crystals / M.E. Wall -- Deuterium labeling for neutron structure-function-dynamics analysis / F. Meilleur, K.L. Weiss and D.A.A. Myles -- Small-angle neutron scattering for molecular biology: Basics and instrumentation / W.T. Heller and K.C. Littrell -- Small-angle scattering and neutron contrast variation for studying bio-molecular complexes / A.E. Whitten and J. Trewhella -- Protein sequencing with tandem mass spectrometry / A.G. Ziady and M. Kinter -- Metabolic analysis / V.V. Tolstikov -- Multicolor detection of combed DNA molecules using quantum dots / C. Escudé [and others] -- Quantum dot molecular beacons for DNA detection / N.C. Cady -- Quantum dot hybrid gel blotting: A technique for identifying quantum dot-protein/protein-protein interactions / T.Q. Vu and H.Y. Liu -- In vivo imaging of quantum dots / I. Texier and V. Josser -- Semiconductor fluorescent quantum dots: Efficient biolabels in cancer diagnostics / P.M.A. Farias, B.S. Santos and A. Fontes -- The monitoring and affinity purification of proteins using dual tags with tetracysteine motifs / R.J. Giannone, Y. Liu and Y. Wang -- Use of genomic DNA as reference in DNA microarrays / Y. Yang -- Single-molecule imaging of fluorescent proteins expressed in living cells / K. Hibino [and others] -- MicroPET, microSPECT, and NIR fluorescence imaging of biomolecules in vivo / Z.-B. Li and X. Chen -- Ultrahigh resolution imaging of biomolecules by fluorescence photoactivation localization microscopy / S.T. Hess [and others] -- Real-time imaging of gene delivery and expression with DNA nanoparticle technologies / W. Sun and A.G. Ziady -- Nanoparticle-mediated gene delivery / S. Jin, J.C. Leach and K. Ye -- Magnetic nanoparticles for local drug delivery using magnetic implants / R. Fernández-Pacheco, J.G. Valdivia and M.R. Ibarra -- Functionalized magnetic nanoparticles as an in vivo delivery system / S. Taira [and others] -- Formulation/preparation of functionalized nanoparticles for in vivo targeted drug delivery / F. Gu, R. Langer and O.C. Farokhzad -- Detection of mRNA in single living cells using AFM nanoprobes / H. Uehara, A. Ikai and T. Osada -- Reverse transfection using gold nanoparticles / S. Yamada [and others] -- Custom-designed molecular scissors for site-specific manipulation of the plant and mammalian genomes / K. Kandavelou and S. Chandrasegaran -- Determining DNA sequence specificity of natural and artificial transcription factors by cognate site identifier analysis / M.S. Ozers, C.L. Warren and A.Z. Ansari

Print version record

Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library

Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002

digitized 2010

HPLC-chip/MS technology in proteomic profiling / M. Vollmer and T. Goor -- Nanofluidic channel fabrication and manipulation of DNA molecules / K.-G. Wang and H. Niu -- A single-molecule barcoding system using nanoslits for DNA analysis / K. Jo, T.M. Schramm and D.C. Schwartz -- Microfluidic devices with photodefinable pseudo-valves for protein separation / Z.H. Fan -- Microfluidic chips designed for measuring biomolecules through a microbead-based quantum dot fluorescence assay / K.-S. Yun [and others] -- DNA focusing using microfabricated electrode arrays / F.A. Shaikh and V.M. Ugaz -- Solid-state nanopore for detecting individual biopolymers / J. Li and J.A. Golovchenko -- Inserting and manipulating DNA in a nanopore with optical tweezers / U.F. Keyser [and others] -- Forming an [alpha]-hemolysin nanopore for single-molecule analysis / N.N. Jetha, M. Wiggin and A. Marziali -- Nanopore force spectroscopy on DNA duplexes / N.N. Jetha, M. Wiggin and A. Marziali -- Quantitative chemical analysis of single cells / M.L. Heien and A.G. Ewing -- Trapping and detection of single molecules in water / M. Willander [and others] -- ZnO nanorods as an intracellular sensor for ph measurements / M. Willander and S. Al-Hilli -- Analysis of biomolecules using surface plasmons / M. Willander and S. Al-Hilli -- Use of residual dipolar couplings in structural analysis of protein-ligand complexes by solution NMR spectroscopy / N.U. Jain -- Raman-assisted x-ray crystallography for the analysis of biomolecules / D. Bourgeois [and others] -- Methods and software for diffuse x-ray scattering from protein crystals / M.E. Wall -- Deuterium labeling for neutron structure-function-dynamics analysis / F. Meilleur, K.L. Weiss and D.A.A. Myles -- Small-angle neutron scattering for molecular biology: Basics and instrumentation / W.T. Heller and K.C. Littrell -- Small-angle scattering and neutron contrast variation for studying bio-molecular complexes / A.E. Whitten and J. Trewhella -- Protein sequencing with tandem mass spectrometry / A.G. Ziady and M. Kinter -- Metabolic analysis / V.V. Tolstikov -- Multicolor detection of combed DNA molecules using quantum dots / C. Escude [and others] -- Quantum dot molecular beacons for DNA detection / N.C. Cady -- Quantum dot hybrid gel blotting: A technique for identifying quantum dot-protein/protein-protein interactions / T.Q. Vu and H.Y. Liu -- In vivo imaging of quantum dots / I. Texier and V. Josser -- Semiconductor fluorescent quantum dots: Efficient biolabels in cancer diagnostics / P.M.A. Farias, B.S. Santos and A. Fontes -- The monitoring and affinity purification of proteins using dual tags with tetracysteine motifs / R.J. Giannone, Y. Liu and Y. Wang -- Use of genomic DNA as reference in DNA microarrays / Y. Yang -- Single-molecule imaging of fluorescent proteins expressed in living cells / K. Hibino [and others] -- MicroPET, microSPECT, and NIR fluorescence imaging of biomolecules in vivo / Z.-B. Li and X. Chen -- Ultrahigh resolution imaging of biomolecules by fluorescence photoactivation localization microscopy / S.T. Hess [and others] -- Real-time imaging of gene delivery and expression with DNA nanoparticle technologies / W. Sun and A.G. Ziady -- Nanoparticle-mediated gene delivery / S. Jin, J.C. Leach and K. Ye -- Magnetic nanoparticles for local drug delivery using magnetic implants / R. Fernandez-Pacheco, J.G. Valdivia and M.R. Ibarra -- Functionalized magnetic nanoparticles as an in vivo delivery system / S. Taira [and others] -- Formulation/preparation of functionalized nanoparticles for in vivo targeted drug delivery / F. Gu, R. Langer and O.C. Farokhzad -- Detection of mRNA in single living cells using AFM nanoprobes / H. Uehara, A. Ikai and T. Osada -- Reverse transfection using gold nanoparticles / S. Yamada [and others] -- Custom-designed molecular scissors for site-specific manipulation of the plant and mammalian genomes / K. Kandavelou and S. Chandrasegaran -- Determining DNA sequence specificity of natural and artificial transcription factors by cognate site identifier analysis / M.S. Ozers, C.L. Warren and A.Z. Ansari

plus-circle Add Review

comment

Reviews