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(19) Japan Patent Office (JP) 

) Kokai Patent Gazette (a) S60- 1 6 1 9 1 1 



Request for 



(43) Publication Date: August 23, 1985 
Not yet requested Number of claims: 1 (Total of 7 pages) 



(54) Title of the Invention Skin Cosmetic 

(21) Appli 



(72) Inventor: 
(72) Inventor: 
(72) Inventor: 
(71) Applicant: 



No.: S59-16238 
(22) Filing Date: January 31, 1984 

Takashi Abe 

294-3, Kamomiya, Odawara-shi 
Tatsu Miyamoto 

19-23-204, Yaezaki-cho, Hiratsuka-shi 
Kiyoshi Maeno 

6-7-17, Togoshi, Shinagawa-ku, Tokyo 
Kanebo, Ltd. 

5-17-4, Sumida, Sumida-ku, Tokyo 



Specification 

1 . Title of the Invention 
Skin Cosmetic 

2. Claims 

(1) A skin cosmetic characterized in comprismg one or 
two or more compounds selected from a group comprising 
cholesteryl sulfate and salts thereof 

(2) The skin cosmetic according to claim (1), 
containing 0.01-5 wt.% of cholesteryl sulfate and/or salts 
thereof 

Tlie present invention relates to a novel skin cosmetic 
that promotes the moisture retention function of the skin, 
comprising one or two or more compounds selected from a 
group comprising cholesteryl sulfate (hereinafter abbreviated 
CHS) and salts thereof 

In the past, in order to maintain liealtliy skin, skin 
cosmetics had been compounded by including water-based 
skin moisturizers and oil-based skin softeners that give the 
skin the proper amounts of moisture and oils. 

The skin moisturizers used include glycerin, propylene 
glycol, polyethylene glycol, pyrrolidone carboxylic acid and 



others, but these may cause the stratum comeum of the 
epidermis (the outennost layer of the skin) to absorb moisture, 
and may conversely cause a loss of moisture from the skin, 
and also, skin cosmetics that contain large amounts of them 
may give a sricky feel, so they had not necessarily been 
satisfactory. 

In addirion, the skm softeners used mclude liquid 
paraffin. Vaseline, olive oil, Scwaran lanolin, synthetic ester 
oils and the like, but these also, when included in a skin 
cosmetic in amounts sufficient to prevent the transpiration of 
moisture from the skin, may cause blockage of the nomial 
metabolism of the skin, among other disadvantages. 

In consideration of the aforementioned disadvantage 
seen in skin softeners, the present inventors conducted 
diligent studies with the object of providing a skin cosmetic 
that, rather than merely depending on such mechanisms as 
supplying moisture to the skin or preventing the transpiration 
of moisture from the skin by the physical action of these 
compounds, would promote the moisture retention function 
that the skin was originally provided with, and keep the skin 
in a healthy state, or repair the skin to that state. 



-63- 



JP S60-161911 A 



As a result, they found that this object is achieved by skin 
cosmetics that contain CHS and/or a salt thereof, thereby 
achieving the present invention. 

To wit, the present invention is a skin cosmetic 
characterized in comprising one or two or more compounds 
selected from a group comprising CHS and salts thereof 

Moisture in the skin constantly travels from the interior 
of the skin toward the outer layers, in accordance with the 
gradient in the moisture content which decreases as one goes 
from the dermis toward the outer layers in the epidermis, 
comprising the basal cell layer and further the stratum 
comeum, and the moisture is ultimately transpired through the 
stratum comeum, but this transpiration of moisture is 
controlled by means of the protective function (barrier 
function) mainly formed from the dense cell structure of the 
stratum corneum. The amount of this transpiration [the 
insensible perspiration (transepidermal water loss) value 
(hereinafter referred to as the "TIVL value") may fall within 
the range of 0.2-0.3 mg/cm^/lir at the epidermis of the 
forearm, for example, with healthy skin in the normal state, 
and normally it is kept below roughly 0.25 mg/cm^/hr. In 
contrast, in the xeroderma (dry skin) states often seen, or in 
dry skin seen with aging skin, the TIVL value may exhibit a 
value near the upper limit of the aforementioned range or 
even a larger value, thus confirming the fact that the moisture 
retention function of the skin is decreased and this derives 
from, in the case of these kinds of dry skin, it is in the state in 
which the normal control limit due to the protection function 
has been exceeded, or this protection function is exhausted. 

Accordingly, if it is possible to make he cell structure of 
the stratum comeum more dense and thus activate this 
protective function, this will promote the skin's water 
retention function and thus the skin will be kept in a healthy 
state and also, moreover it will be possible to ameliorate or 
repair dry skin, and as a result of the studies of the present 
inventors, it became clear that a skin cosmetic that contains 
CHS and/or salts thereof normalizes these intercellular 
functions of the skin and makes the structure of the corneal 
cells denser so that the moisture retention function of the skin 
can be promoted. 

For this reason, the present invention provides a skin 
cosmetic that, by promoting the moisture retention function of 
the skin itself can ameliorate the dry skin condition, or keep 
the skin in the healthy condition and prevent its aging, and 
thus give tlie skin moistness (silky feel), flexibility (smooth 
feel) elasticity and glow. 



In addition, in the case of the skin cosmetic according to 
the present invention, there is no need to use large amounts of 
moisturizers and softeners as in conventional skin cosmetics, 
so there is no risk of these compounds impeding the normal 
physiological functions of the skin. 

In order to obtain the CHS and salts thereof to be used in 
the skin cosmetic according to the present invention, it is 
sufficient to use known methods, to wit, by dissolving 
cholesterol in formamide, dimethyl formamide, pyridine or 
other basic organic solvents, or in benzene, toluene or other 
organic solvents, and then reacting with anhydrous sulfuric 
acid, fuming sulfuric acid, chlorosulfonic acid, a pyridine- 
anhydrous sulfuric acid complex or other sulfuric acid 
esterifying agent, and the impure CHS thus obtained may be 
used as is, or in the form of a desired salt obtained by 
allowing various bases to act thereupon, and thus produced by 
the ordinary methods of fractional precipitation and 
manipulation using methanol, acetone, benzene, petroleum 
ether, purified water and the like. 

Here, the usable salts of CHS include any of those that 
can be stably incorporated within a skin cosmetic base and 
that are harmless from a physiological standpoint, but to 
present several columns that are particularly preferable, these 
include, for example, as inorganic salts: sodium salt, calcium 
salt, magnesium salt, etc. and as salts with organic bases: 
lysine salts, aspartates, triethanol amine salts and the like. In 
addition, these salts may include, for sodium salts for example, 
the dihydrate or hexahydrate forms, with hydrates in various 
forms, and naturally these may be similarly used. Note that 
Table 1 presents examples of the CHS and salts thereof 



Table 1 



CHS and Salts Thereof 


Rational Formula 


Melting or 
Decomposition 
Point (°C) 


CHS 

Sodium salt (dihydrate) 
Potassium salt 
(monohydrate) 
Calcium salt 
Magnesium salt 


C27H45SO4H 
C27H45S04Na-2H20 

C27H45S04K-H20 
(C27H45S04)2Ca 
(C27H45S04)2Mg 


72-74 
163-167 
202-208 

134-138 
137-143 



With the present invention, CHS or salts thereof are 
incorporated into a skin cosmetic base as a single salt or a 
mixture of two or more salts. In addition, depending on the 
case, it is also possible to use CHS which is partially 
neutralized with an equal amoimt or less of a base. 



-64- 



JPS60-161911 A 



The content of the CHS or salts thereof incorporated, as 
the total content of the on or two or more compounds thereof 
is typically in the range 0.0 1-5 wt.% of the total weight of the 
skin cosmetic, and preferably in the range 0.05-3 wt.%. If the 
content is less than 0.01 wt.%, the meritorious effect of the 
skin cosmetic according to the present invention will not be 
adequately achieved, and on the other hand if the content is in 
excess of 10 wt.%, there is no prospect for any further 
increase in the meritorious effect concomitant to the amount 
of increase. 

The skin cosmetic according to the present invention 
containing this CHS and/or salts thereof may be manufactured 
by normal methods. To wit, it is sufficient to dissolve, 
disperse or uniformly mix one or two or more compounds 
selected from the group comprising CHS and the salts thereof 
into a desired skin cosmetic base, thus obtaining a lotion, 
milky lotion, cream or pack. 

In this case, it is preferable for the sodium salts, 
potassium salts, lysine salts, triethanol amine salts or other 
salts of CHS to be uniformly mixed as an aqueous solution, 
while the CHS and the calcium salts, magnesium salts, 
aspartates and other salts thereof to be uniformly dissolved or 
dispersed in an oil-based base. 

Here follows a detailed description of the present 
invention made with reference to working examples and 
experimental examples thereof 

Note that the method of measuring and the method of 
evaluating the TWL value, percentage change in the TWL 
value and exfoliation characteristics of corneal cells are 
presented below. 

(1) TTFi Value 

We used a method whereby the change in the humidity 
of a sealed volume of air above the skin is measured using its 
electrical resistance. 

To wit, we sealed a measurement cell to the skin of the 
experimental subject, forced dry air through the cell and thus 
adequately replaced the air within the cell with dry air, and 
then halted the supply of dry air and found the relative 
humidity within the cell at that time as RHs (%>), left the cell 
alone for 10 minutes and again measured the relative 
humidity within the cell as RHio (%). The TWL value was 
calculated from the change in humidity values at this time by 
the following formula: 



TWL 



.(^^--^->^--^-^(mg/cm'/hr) 



Where, 

D,: density of saturated water vapor in air at the measured 

temperature (mg/L) 
V: volume of the cell (L) 
S: surface area, measured (cm*) 

(2) Percentage Change in TWL Value 

We measured the TWL value before and after applying a 
sample (skin cosmetic) to the skin and calculated the 
percentage change therein by the following formula: 
TWL, -TWL, 



Percentage change in TWL = 



TWL„ 



xlOO(%) 



Where, 

TWLj. TWL value before application of sample (skin 
cosmetic) 

TWL/j-. TWL value after application of sample (skin cosmetic) 

(3) Exfoliation Property of Corneal Cells 

We applied Scotch tape (Nichiban mending tape) to the 
skin, removed the tape and thus caused corneal cells of the 
skin to be adhered to the tape. Next, we performed a detailed 
observation of the state of these corneal cells using a scanning 
electron microscope and, based on the criteria presented in 
Table 2, found an index value after determining the 
appropriate category for the exfoliation property of the 
corneal cells. 

Table 2 



State of Exfoliated Corneal Cells 


Index Value 


No scales found 

Scattered small scales found 

Scattered small to medium scales found 

Conspicuous small to medium scales found 

Conspicuous large scales found 


1 

2 
3 
4 
5 



Note that the exfoliation property of these corneal cells 
becomes an index for determining the structure of the stratum 
comeum, and typically in dry skin and aged skin, it is though 
that the amount of intercellular binding is though to be weak, 
and the density of its structure is low, so this index value is 
thought to increase. 

[Seal] Remainder Left Blank 



65- 



JP S60-161911 A 



4 and Comparative Example 1 (Skin Cream) 



Ingredients 


Content (wt.%) 


Comparative Example 


Working Examples 


! 


! 




? 






/^Beeswax 


2.0 


2.0 


1 

2.0 


2.0 


T— 

2.0 




Stearic acid 


5.0 












Stearyl alcohol 




3 0 


3 0 


3 0 


3 0 




Reduced Lanolin 


2 0 


2.0 


2.0 


2.0 


2.0 


(AX 


Scwaran 


15.0 


15.0 


15.0 


15.0 


15.0 




Sorbitan monostearate 


3.0 


3.0 


3.0 


3.0 


3,0 




Polyoxyethylene sorbitan monostearate 


3.0 


3.0 


3.0 


3.0 


3.0 




CHS 




0.5 










Calcium salt of CHS 






0.5 








Sodium salt of CHS (dihydride) 








0.5 






Lysine salt of CHS 










0.5 


(B)< 


Propylene glycol 


5.0 


5.0 


5.0 


5.0 


5.0 




Preservative 


0.2 


0.2 


0.2 


0.2 


0.2 




^ Purified water 


61.75 


61.25 


61.25 


61.25 


61.25 


(C) 


Perfume 


0.05 


0.05 


0.05 


0.05 


0.05 



(2) Method of Prepi 

Component (A) and component (B) were melted by 
heating each to 80''C, and then were cooled while stirring, and 
component (C) was added when they reached 50°C; stirring 
was continued until 30°C was reached, thus obtaining creams 
of each. 

Experimental Example 1 

We examined the TWL values of Comparative Example 
1 and Working Example 1 of the present invention, and the 
effects they have on the corneal cell exfoliation property. 
(1) Test Method 

We assembled Group A as 20 test subjects (women in 
the age range of 20-25 years) that have normal healthy skin. 
Group B as 20 test subjects (women in the age range of 20-25 
years) that exhibit normal dry skin, and Group C as 20 test 
subjects (women in the age range of 50-60 years) that exhibit 
dry skin due to aging, and measured the TWL value of the 
forearm skin of each subject in advance. 

Next, all of the test subjects in Groups A, B and C were 
asked to apply the skin cream of Comparative Example 1 to 
the skin of their left forearm, and the skin cream of Working 
Example 1 of their right forearm twice daily (morning. 

Table 3 



ig), continuously for one month. On the next day after 
the final application, the TWL values of all test subjects' left 
and right forearms where the cream had been applied were 
measured, and moreover the corneal cell exfoliation property 
thereof was also evaluated. 

Note that as a result of examinations performed by 
physicians after the end of the application test, there were no 
abnormalities found with respect to the forearm skin or health 
of any of the test subjects. 
(2) Results 

Table 3 presents the TWL values before and after 
application of skin cream (average values of the 20 test 
subjects in each Group), the percentage change in the TWL 
value found from these TWL values, along with the index 
values for the corneal cell exfoliation property (average 
values of the 20 test subjects in each Group). 

Note that in Table 3, the TWL values prior to applying 
cream increased in Group A, Group B and Group C in this 
order, and this indicates that the loss of moisture from the skin 
increases in this order, and so the density and function of the 
corneal cells decreases concomitantly. 



Test Subjects and Forearm 
Locations 




7WZ, Value (mg/cm^/hr) 


Percentage 


Corneal Cell 


Sample Applied 


Before Applying 


After Applying 


change in TWL 
value (%) 


Exfoliation 
Property 


Group A 

Healthy skin 

(20 subjects) 


Left 


Comparative Example 1 


0.230 


0.225 


2.2 


1.7 


Right 


Working Example 1 


0.240 


0.220 


8.3 


1.4 


Group B 

Dry skin 

(20 subjects) 


Left 


Comparative Example 1 


0.300 


0.282 


6.0 


2.7 


Right 


Working Example 1 


0.310 


0.211 


31.9 


1.8 


Group C 
Age-deteriorated skin 
(20 subjects) 


Left 


Comparative Example 1 


0.515 


0.475 


7.8 


3.6 


Right 


Working Example 1 


0.530 


0.290 


45.3 


2.1 



Based on the results illustrated in Table 3 the rr 
effects of the skin cream of Working Example 1 of the present 
invention were confirmed as follows. 

(1) On the normal healtliy skin in Group A, the moisture 
retention function of the original skin and the structural 
characteristics of the corneal cells were kept in the normal 



state, so the effect of applying the skin cream according to 
the present invention (Working Example 1) is not readily 
evident, but still there was slight improvement in 
comparison to Comparative Example 1. 
(2) On the dry skin of Groups B and C, there was a marked 
improvement in the TWL val ue of the right forearm . . . 



-66- 



JP S60-161911 A 



... skin where the skin cream according to the present 
invention (Working Example 1) was applied, in 
comparison to the left forearm skin (where the skin cream 
of Comparative Example 1 was applied), and the value 
thereof was equal to or close to that of normal skin. In 
addition, the skin cream according to the present 
invention was slightly better with respect to the corneal 
cell exfoliation property also. 
To wit, the skin cream according to Working Example 1 of 
the present invention exhibited the meritorious effect of 
maintaining healthy skin in Group A, and in Groups B and C, 
it clearly had a meritorious effect of bringing it closer to 
healthy skin. 

Based on these results, one can see that the CHS 
contained in the skin cream according to Working Example 1 
of the present invention effectively acted on the cells in the 
stratum corneum, thus improving the intercellular binding 
ability in the stratum comeum and making its structure denser, 
thereby activating the protective function of the stratum 
comeum and promoting the moisture retention function of the 
skin, and yet it is still highly safe without impairing the 
physiological functions ofhealthy skin. 
Experimental Example 2 

We examined the change in the TWL values when the 
skin creams according to Working Examples 2, 3 and 4 of the 
present invention as well as the skin cream according to 
Comparative Example 1 were applied t) dry skin. 



(1) Test Method 

We assembled 60 new test subjects that exhibited normal 
dry skin (women in the age range of 26-34 years) and divided 
them into three groups (Groups D, E and F). Prior to the start 
of testing, we measured the TWL value of the left and right 
forearm skin of all test subjects, and calculated the average 
value for each Group. 

Next, all of the test subjects in Groups A, B and C were 
asked to apply the skin cream of Comparative Example 1 to 
the skin of their left forearm, and a different skin cream for 
each Group to the skin of their right forearm twice daily 
(morning, evening), continuously for one month, with Group 
D applying the skin cream of Working Example 2, Group E 
applying that of Working Example 3 and Group F applying 
that of Working Example 4, respectively. On the next day 
after the final application, the TWL values of all test subjects' 
left and right forearms where the cream had been applied 
were measured. 

Note that as a result of examinations performed by 
physicians after the end of the application test, there were no 
abnormalities found with respect to the forearm skin or health 
of any of the test subjects. 

(2) Results 

Table 4 presents the TWL values before and after 
application of skin cream (average values of the 20 test 
subjects in each Group) and the percentage change in the TWL 
value found from these TWL values. 



Test Subjects and Forearm 
Locations 


Sample Applied 


3m Value (mg/cm^/hr) 


Percentage 
change in TWL 
value (%) 


Before Applying 
Cream 


After Applying 
Cream 


Group D (20 subjects) 


Left 


Comparative Example 1 


0.290 


0.273 


5.8 


Right 


Working Example 2 


0.310 


0.226 


27.1 


Group E (20 subjects) 


Left 


Comparative Example 1 


0.295 


0.283 


4.1 


Right 


Working Example 3 


0.284 


0.202 


29.0 


Group C (20 subjects) 


Left 


Comparative Example 1 


0.305 


0.281 


7.9 


Right 


Working Example 4 


0.308 


0.203 


34.1 



With the results presented in Table 4, we confirmed that 
the cream containing sodium salt of CHS, potassium salt of 
CHS and lysine salt of CHS in Working Examples 2-A 
reduced the TWL values in the same manner as the skin cream 
containing CHS in Working Example 1, normalized the 
protective function of the stratum comeum and also promoted 

Experimental Example 3 

We assembled groups of 30 test subjects each (women in 
the age range of 26-34 years) that complained of dry skin and 
asked them to apply the skin creams of Comparative Example 



1 and Working Examples 1-4 twice daily (morning, evening) 
continuously for one month. As a result of examinations 
performed by physicians after the end of the application test, 
there were no abnormalities found with respect to the forearm 
skin or health of any of the test subjects. 

Table 5 presents the results of interviewing all test 

; (silky feel), flexibility (smooth feel), elasticity and 
after applying the cieam. 

[Seal] Remainder Left Blank 



-67- 



JP S60-1619I1 A 







Samples Applied 


Item 


Evaluation 


Comparative Example 


Working Examples 






• 


2 


3 






Extremely good 


0 replies 


19 replies 


18 replies 


19 replies 


17 replies 




Good 


3 


6 


10 


5 




Skin moistness 


Somewhat good 


6 


3 










No change 


20 


2 


0 


2 


3 




Somewhat poor 


1 


0 


0 








Extremely good 


0 


18 


19 


16 


17 




Good 


6 


5 


6 


5 


5 


Flexibility 


Somewhat good 


5 


^ 


^ 








No change 


19 












Somewhat poor 




0 


0 


0 


0 




Extremely good 


0 


15 


16 


19 


18 




Good 


1 


7 


5 


6 


7 


Elasticity 


Somewhat good 


4 


5 


6 


4 


3 




No change 


24 


3 


3 


1 


2 




Somewhat poor 


1 


0 


0 


0 


0 




Extremely good 


0 






20 


17 




Good 


2 




6 


4 


6 


Glow 


Somewhat good 


6 


3 


4 


5 


5 




No change 


22 


1 


2 




2 




Somewhat poor 


0 


0 


0 


0 


0 



As is evident from the results of Table 5, the skin creams 
of Working Examples 1^ which contain CHS and salts 
thereof demonstrated effects that were markedly superior to 
the skin cream according to Comparative Example 1 . 
Working Examples 5-8 and Comparative Example 2 (Skin 
Lotions) 

We prepared a skin lotion (Comparative Example 2) 
according to the ordinary method using the following as the 
raw ingredients: 10 wt.% of ethanol, 3 wt.% of glycerin, 

Table 



0.2 wt% of polyoxyethylene sorbitan m 
(solubilizer), 0.01 wt.% of preservative, 0.01 wt.% of perfume, 
artificial color and added purified water in the remaining 
amount to reach 100 wt.%. Next, we similarly prepared skin 
lotions (Working Examples 5-8) using the same raw 
ingredients as for Comparative Example 2, but adding sodium 
salt of CHS (dihydride) in the amounts indicated in Table 6 to 
reach 100 wt%. 

[Seal] Remainder Left Blank 



Comparative Examples and Working Examples 


Contentof Sodium Salt of CHS (dihydride) (wt.%) 


Comparative Example 2 


0 


Working Example 5 


0.01 


Working Example 6 


1.0 


Working Example 7 


3.0 


Working Example 8 


5.0 



Experimental Example 

We assembled groups of 30 test subjects each 
the age range of 26-34 years) that complained of dry 
asked them to apply the skin lotions of Comparati 
2 and Working Examples 5-8 twice daily (morning, 
continuously for one month. As a result of 
performed by physicians after the end of the appli 



skin and 
Example 
evening) 



there were no abnormalities found with respect to the forearm 
skin or health of any of the test subjects. 

Table 7 presents the results of interviewing all test 
subjects in regard to the meritorious effects of giving skin 
moistness (silky feel), flexibility (smooth feel), elasticity and 
glow after applying the cream. 



JPS60-16191I A 







Samples Applied 


Item 


Evaluation 


Comparative 


Example 


Working Examples 
















■ 




Extremely good 


0 r 


eplies 


0 replies 


1 1 replies 


23 replies 


24 replies 




Good 


1 




7 








Skin moistness 


Somewhat good 






13 










No change 


23 




10 


3 


0 


0 




Somewhat poor 


3 




0 


0 








Extremely good 


0 




0 


10 


16 


16 




Good 


0 




5 


8 


1 1 


12 


Flexibility 


Somewhat good 


4 














No change 






's 










Somewhat poor 


^2 




1 


0 


0 


0 




Extremely good 


0 




0 


12 


21 


22 




Good 


0 




6 


9 


7 


7 


Elasticity 


Somewhat good 


3 




15 


6 


2 


1 




No change 


23 




7 


3 


0 


0 




Somewhat poor 


4 




2 


0 


0 


0 




Extremely good 


0 




0 


13 


22 


21 




Good 


0 




7 


8 


6 


7 


Glow 


Somewhat good 


6 




16 


7 




2 




No change 


21 




6 


2 


1 


0 




Somewhat poor 


3 






0 


0 


0 



As is evident from the results of Table 7, each of the skin prepared by neutralizing CHS in triethanol 
lotions according to Working Examples 5-8 of the present 
invention demonstrated effects that were superior to those of 
Comparative Example 2, and particularly superior skin 
improvement effects were observed in the case of the skin 
lotions of Comparative Examples 6-8 in particular. 

Note that the skin lotion of Working Example 8 
exhibited a slight tendency to gel in long-term storage. 
Working Example 9 and Comparative Example 5 (Skin Packs 
(Membrane Type)) 

We prepared a skin pack (membrane type) (Comparative 
Example 3) according to the ordinary method using the 
following as the raw ingredients: 10 wt.% of polyvinyl 
alcohol (average degree of polymerization: 1750, degree of 
saponification: 88 mol%), 3 wt.% of glycerin, 0.02 wt.% of 
perfume, 0.05 wt.% of preservative, artificial color and added 
purified water in the remaining amount to reach 100 wt.%. 
Next, we similarly prepared a skin pack (membrane type) 
(Working Example 9) using the same raw ingredients as for 
Comparative Example 3, but adding 0.2 wt.% of a salt 



o pH=7.0 



Table 8 



Experimental Example 5 

We assembled groups of 50 test subjects each (women in 
the age range of 20-60 years) and asked them to apply the 
skin packs of Comparative Example 3 and Working Example 
9 twice daily (morning, evening) continuously for one month. 
As a result of examinations performed by physicians after the 
end of the application test, there were no abnormalities found 
with respect to the forearm skin or health of any of the test 
subjects. 

We interviewed all test subjects in regard to the 
meritorious effects of giving skin moistness (silky feel), 
flexibility (smooth feel), elasticity and glow after applying the 
skin packs (membrane type), and the numbers of subjects that 
replied to the effect that improvements occurred are presented 
in Table 8. The skin pack (membrane type) according to 
Working Example 9 was found to have clearly markedly 
superior meritorious effects in comparison to that of 
Comparative Example 3. 

[Seal] Remainder Left Blank 



Item 


Comparative Example 3 


Working Example 9 


Number of subjects with improved moistness 


11 out of 50 


41 out of 50 


Number of subjects with improved flexibility 


8 out of 50 


36 out of 50 


Number of subjects with improved elasticity 


4 out of 50 


33 out of 50 


Number of subjects with improved glow 


3 out of 50 


36 out of 50 



Patent Applicant: Kanebo, Ltd. [Seal] 



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