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Full text of "USPTO Patents Application 09804625"

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What is claimed is: 

1. An osteogenic protein comprising an amino acid sequence 
selected from the group consisting of: 

(a) amino acids #32-#129 of Figure 1 (SEQ ID NO: 2) ; 

(b) amino acids #299-#396 of Figure 2 (SEQ ID NO: 4); and 

(c) amino acids #311-#408 of Figure 3 (SEQ ID NO: 6) . 



2. An osteogenic protein selected from the group consisting of: 

(a) a purified BMP-2 protein produced by the steps of 

(i) culturing a cell transformed with 

(a) a cDNA comprising the nucleotide sequence 
from nucleotide #356 to #1543 as shown in Figure 

2 (SEQ ID NO: 3) ; and 

(b) sequences which hybridize to sequence (a)^ 
under stringent conditions and encode a 
protein having cartilage and/or bone induction 
activity 

(ii) recovering and purifying from said culture 
medium a protein comprising the 97 amino acid 
sequence from amino acid *#299 to amino acid #396 as 
shown in Figure 2 (SEQ ID NO: 4) ; and 

(b) a purified BMP-4 protein produced by the steps of 

(i) culturing a cell transformed with 

(a) a cDNA comprising the nucleotide sequence 
from nucleotide #403 to #1626 as shown in Figure 

3 (SEQ ID NO: 5) ; and 

(b) sequences which hybridize to sequence (a) 
under stringent conditions and encode a 
protein having cartilage and/or bone induction 
activity; 

(ii) recovering from said culture medium a protein 
comprising the 97 amino acid sequence from amino acid 
#311 to amino acid #408 as shown in Figure 3 (SEQ ID 
NO: 6), 



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3. A BMP-2 protein comprising amino acids #283-#396 of Figure 

2 (SEQ ID NO: 4) • , 

4. The protein of claim 3 wherein said protein is a disulfide 
linked dimer wherein at least one of the subunits of said dimer 
comprises amino acid #283-#396 of Figure 2 (SEQ ID NO: 4) . 

5. A purified BMP-2 protein produced by the steps of 

(a) culturing a cell transformed with a cDNA comprising 
the nucleotide sequence from nucleotide #356 to #1543 as 
shown in Figure 2 (SEQ ID NO: 3); and 

(b) recovering and purifying from said culture medium a 
protein comprising the 97 amino acid sequence from amino 
acid #299 to amino acid #396 as shown in Figure 2 (SEQ ID 
NO: 4). 

6. A BMP-4 protein comprising amino acids #293-#408 of Figure 

3 (SEQ ID NO: 6) . 

7. The protein of claim 6 wherein said protein is a disulfide 
linked dimer wherein at least one of* the subunits of said dimer 
comprises amino acid #293-#408 of Figure 3 (SEQ ID NO: 6). 

8. A purified BMP-4 protein produced by the steps of 

(a) culturing a cell transformed with a cDNA comprising 
the nucleotide sequence from nucleotide #403 to #1626 as 
shown in Figure 3 (SEQ ID NO: 5) ; and 

(b) recovering form said culture medium a protein 
comprising the 97 amino acid sequence from amino acid #311 
to amino acid #408 as shown in Figure 3 (SEQ ID NO: 6) . 

9. A protein of claim 1 further characterized by the ability 
to induce the formation of cartilage and/or bone, 

10- A pharmaceutical composition comprising an effective 



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amount of a BMP-2 protein in admixture with a pharmaceutical]^ 
acceptable vehicle. , 

11. A pharmaceutical composition comprising an effective 
amount of a BMP-4 protein in admixture with a pharmaceutical^ 
acceptable vehicle. 

12. A composition for bone and/or cartilage formation 
comprising an effective amount of a BMP-2 protein in a 
pharmaceutical^ acceptable vehicle. 

13. A composition for bone and/ or cartilage formation 
comprising an effective amount of a BMP-4 protein in a 
pharmaceutical ly acceptable vehicle. 

14. A composition of claim 12 further comprising a matrix for 
supporting said composition and providing a surface for bone 
and/or cartilage growth. 

15. A composition of claim 13 further comprising a matrix for 
supporting said composition and prdviding a surface for bone 
and/or cartilage growth. 

16. The composition of claim 14 wherein said matrix comprises 
a material selected from the group consisting of 
hydroxyapatite, collagen, polylactic acid and tricalcium 
phosphate. 

17. The composition of claim 15 wherein said matrix comprises 
a material selected from the group consisting of 
hydroxyapatite, collagen, polylactic acid and tricalcium 
phosphate. 

18. A method for inducing bone and/or cartilage formation in 
a patient in need of same comprising administering to said 



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patient an effective amount of the composition of claim 11. 

19. A method for inducing bone and/ or cartilage formation in 
a patient in need of same comprising administering to said 
patient an effective amount of the composition of claim 11. 

20. A pharmaceutical composition for wound healing and tissue 
repair said composition comprising an effective amount of a 
BMP-2 protein in a pharmaceutical^ acceptable vehicle. 

21. a pharmaceutical composition for wound healing and tissue 
repair said composition comprising an effective amount of a 
BMP-4 protein in a pharmaceutical^ acceptable vehicle. 

22. A method for treating wounds and/or tissue repair in a 
patient in need of same comprising administering to said 
patient an effective amount of the composition of claim 21. 

23. A method for treating wounds and/or tissue repair in a 
patient in need of same comprising administering to said 
patient an effective amount of the composition of claim 22.