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Full text of "USPTO Patents Application 09804625"

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September 12, 1989 which is a continuation-in-part of 07/655,579 filed March 18, 1991 which is 
a divisional of 07/179,100 filed April 8, 1988 (US Patent 5,013,649) which is a continuation- in- part 
of 07/028,285 filed March 20, 1987( abandoned) ;which is a continuation- in-part of 06/943,332 filed 
December 17, 1986 (abandoned) which is a continuation-in-part of 06/880,776 filed July 1, 1986, 
abandoned... 



In the Claims: 



24. An isolated DNA molecule comprising a DNA sequence selected fi*om the group consisting 



(a) nucleorides 1202 through 1543 of Figure 2; 

(b) nucleotide 1252 through 1543 of Figure 2; 

(c) nucleotides 1279 through 1626 of Figure 3; 

(d) nucleotides 1333 through 1626 of Figure 3; 

(e) nucleotides encoding amino acids 283 through 396 of Figure 2; 

(f) nucleotides encoding amino acids 299 through 396 of Figure 2; 

(g) nucleotides encoding amino acids 293 dirough 408 of Figure 3; 

(h) nucleotides encoding amino acids 3 1 1 through 408 of Figure 3; and 

(i) naturally occurring allelic sequences and equivalent degenerative codon sequences 



of (a) through (h). 

25. An isolated DNA molecule comprising a DNA sequence of nucleotides 1202 through 1543 
of Figure 2. 



Please 




new claims as follows: 



USSN TBD 

Filed March 9, 2001 

Page 3 

26. An isolated DNA molecule comprising a DNA sequence of nucleotide 1252 through 1543 
of Figure 2. 

27. An isolated DNA molecule comprising a DNA sequence of nucleotides 1279 through 1626 
of Figure 3. ^ - - - 

28. .An isolated DNA molecule comprising a DNA sequence of nucleotides 1333 through 1626 
of Figure 3. 

29. An isolated DNA molecule comprising a DNA sequence of nucleotides encoding amino acids 
283 through 396 of Figure 2. 

30. An isolated DNA molecule comprising a DNA sequence of nucleotides encoding amino adds 
299 du-ough 396 of Figure 2, 

31. An isolated DNA molecule comprising a DNA sequence of nucleotides encoding amino acids 
293 dirough 408 of Figure 3 . 

32. An isolated DNA molecule comprising a DNA sequence of nucleotides encoding amino acids 
3 1 1 through 408 of Figure 3 . 

33. An isolated DNA sequence encoding BMP-2 protein which hybridizes to a DNA sequence 
of claim 30 under stringent hybridization conditions. 

34. .An isolated DNA sequence encoding BMP-4 protein which hybridizes to a DNA sequence 
of claim 32 under stringent hybridization conditions. 

35. A vector comprising a DNA molecule of claim 24 in operative association with an expression 
control sequence therefor. 

36. A vector comprising a DNA molecule of claim 33 in operative association with an expression 
control sequence therefor. 



USSN TBD 

Filed March 9, 2001 

Page 4 

37. A vector comprising a DNA molecule of claim 34 in operative association with an expression 
control sequence therefor. 

38. A host cell transformed with a vector of claim 35. 

39. A host cell transformed with a vector of claim 36. 

40. A host cell transformed with a vector of claim 37. 

41. An isolated DNA molecule comprising a DNA selected from the group consisting of ATCC 
deposits 40345 and 40342. 

42. A method for producing a BMP-2 protein said method comprising the steps of: 

(a) culturing a host cell transformed with a DNA sequence of claim 33, and; 

(b) recovering said BMP-2 protein. 

43. A method for producing BMP-4 protein, said method comprising the steps of: 

(a) culturing a host cell transformed with a DNA sequence of claim 34, and; 

(b) recovering said BMP-4 protein. 

44. A BMP-2 made by the method of claim 42, 

45. A purified BMP-2 polypeptide comprising an amino acid sequence encoded by a DNA 
sequence comprising nucleotides 1202 through 1543 of Figure 2. 

' AT. A purified BMP-2 polypeptide comprising an amino acid sequence encoded by a DNA 
sequence comprising nucleotides 1252 through 1543 of Figure 2. 



^ 49. A purified BMP-2 protein comprising an amino acid sequence of amino acid 299 through 396 
of Figure 2. 




A purified BMP-2 protein comprising an amino acid sequence of amino acid 283 through 396 



of Figure 2.