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OIPE 



. . PAGE: 1 



RAW SEQUENCE LISTING 

PATENT APPLICATION US/09/804, 62S 



DATE: 01/1 1/-2002 
TIME: 22:10:28 



INPUT SET: S3671S.raw 



This Raw Listing contains the General 
Information Section and up to the first 5 pages. 



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SEQUENCE LISTING 



(1) 



General Information : 



(i) APPLICANT: Wang, Elizabeth A. 

Rosen, Vicki A. 
Wozney, John M 

(ii) TITLE OF INVENTION: Novel BMP Products 



ENTERED 



(iii) NUMBER OF SEQUENCES: 6 

(iv) CORRESPONDENCE ADDRESS: 

(A) ADDRESSEE: LEGAL AFFAIRS, GENETICS INSTITUTE, INC. 

(B) STREET: 87 CAMBRIDGEPARK DRIVE 

(C) CITY: CAMBRIDGE 

(D) STATE: MA 

(E) COUNTRY: USA 

(F) ZIP: 02140 

(v) COMPUTER READABLE FORM: 

(A) MEDIUM TYPE: Floppy disk 

(B) COMPUTER: IBM PC compatible 

(C) OPERATING SYSTEM: PC-DOS/MS -DOS 

(D) SOFTWARE: Patentin Release #1.0, Version #1.25 



(vi) CURRENT APPLICATION. DATA: 

(A) APPLICATION NUMBER: 

(B) FILING DATE: 

(C) CLASSIFICATION: 



09/804, 625 



(vii) PRIOR APPLICATION DATA: 

(A) APPLICATION NUMBER: 08/925,779 

(B) FILING DATE: 



(viii) ATTORNEY/AGENT INFORMATION: 

(A) NAME: Kapinos , Ellen J. 

(B) REGISTRATION NUMBER: 32,245 

(C) REFERENCE/DOCKET NUMBER: 5160C 

(ix) TELECOMMUNICATION INFORMATION: 

(A) TELEPHONE: 617-876-1170 

(B) TELEFAX: 617-876-5851 




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47 (2) INFORMATION FOR SEQ ID NO : 1 : 
48 

49 (i) SEQUENCE CHARACTERISTICS: 

50 (A) LENGTH: 592 base pairs 

51 (B) TYPE: nucleic acid 

52 (C) STRANDEDNESS : double 

53 (D) TOPOLOGY: linear 
54 

55 (ii) MOLECULE TYPE: DNA (genomic) 

56 

57 (iii) HYPOTHETICAL: NO 

' 58 

59 (iv). ANTI-SENSE: NO 

60 

61 (vi) ORIGINAL SOURCE: 

62 (A) ORGANISM: BOS TAURUS 
63 

64 (vii) IMMEDIATE SOURCE: 

65 (A) LIBRARY: BOVINE GENOMIC IN LAMBDA Jl 

66 (B) CLONE: LAMBDA BP-21 

67 - 

68 (viii) POSITION IN GENOME: 
6 9 (C) UNITS: bp 

70 

71 (ix) FEATURE: 

72 (A) NAME/KEY: CDS 

73 , (B) LOCATION: 1..390 
74 

75 (ix) FEATURE: 

76 (A) NAME/KEY: mat_J3eptide 

77 (B) LOCATION: 46.. 387 

78 . 
79 

80 (xi) SEQUENCE DESCRIPTION: SEQ ID N0:1: 

81 

82 GGC CAC GAT GGG AAA GGA CAC CCT CTC CAC AGA AGA GAA AAG CGG CAA 48 

83 Gly His Asp Gly Lys Gly His Pro Leu His Arg Arg Glu Lys Arg Gin 

84 -15 -10 -5 1 
85 

86 GCA AAA CAC AAA CAG CGG AAA CGC CTC AAG TCC AGC TGT AAG AGA CAC 96 

87 Ala Lys His Lys Gin Arg Lys Arg Leu Lys Ser Ser Cys Lys Arg His 

88 5 10 15 
89 

90 CCT TTA TAT GTG GAC TTC AGT GAT GTG GGG TGG AAT GAC TGG ATC GTT 144 

91 Pro Leu Tyr Val Asp Phe Ser Asp Val Gly Trp Asn Asp Trp lie Val 

92 20 25 30 
93 

94 GCA CCG CCG GGG TAT CAT GCC TTT TAC TGC CAT GGG GAG TGC CCT TTT 192 

95 Ala Pro Pro Gly Tyr His Ala Phe Tyr Cys His Gly Glu Cys Pro Phe 

96 35 40 45 
97 

98 CCC CTG GCC GAT CAC CTT AAC TCC ACG AAT CAT GCC ATT GTC CAA ACT 240 

99 Pro Leu Ala Asp His Leu Asn Ser Thr Asn His Ala lie Val Gin Thr 



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100 50 55 60 65 

101 

102 CTG GTC AAC TCA GTT AAC TCT AAG ATT CCC AAG GCA TGC TGT GTC CCA 2 88 

103 Leu Val Asn Ser Val Asn Ser Lys lie Pro Lys Ala Cys Cys Val Pro 

104 70 75 80 
105 

106 ACA GAG CTC AGC GCC ATC TCC ATG CTG TAG CTT GAT GAG AAT GAG AAG 33 6 

107 Thr Glu Leu Ser Ala lie Ser Met Leu Tyr Leu Asp Glu Asn Glu Lys 

108 85 90 95 
109 

110 GTG GTA TTA AAG AAC TAT CAG GAC ATG GTT GTC GAG GGT TGT GGG TGT 384 

111 Val Val Leu Lys Asn Tyr Gin Asp Met Val Val Glu Gly Cys Gly Cys 

112 100 105 110 
113 

114- CGT TAGCACAGCA AAATAAAATA TAAATATATA TATATATATA TTAGAAAAAC 437 

115 Arg 

116 115 
117 

118 AGCAAAAAAA TCAAGTTGAC ACTTTAATAT TTCCCAATGA AGACTTTATT TATGGAATGG . 497 

119 - 

120 AATGGAGAAA AAGAAAAACA CAGCTATTTT GAAAACTATA TTTATATCTA CCGAAAAGAA 557 
121 

122 GTTGGGAAAA CAAATATTTT AATCAGAGAA TTATT 592 

123 

124 

125 (2) INFORMATION FOR SEQ ID NO : 2 : 
126 

127 (i) SEQUENCE CHARACTERISTICS: 

128 (A) LENGTH: 12 9 amino acids 

129 (B) TYPE: amino acid 

130 (D) TOPOLOGY: linear 
131 

132 (ii) MOLECULE TYPE: protein 

133 

134 (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 2 : 

135 

136 Gly His Asp Gly Lys Gly His Pro Leu His Arg Arg Glu Lys Arg Gin 

137 -15 -10 -5 1 
138 

139 Ala Lys His Lys Gin Arg Lys Arg Leu Lys Ser Ser Cys Lys Arg His 

140 5 10 15 
141 

142 Pro Leu Tyr Val Asp Phe Ser Asp Val Gly Trp Asn Asp Trp lie Val 

143 20 25 30 
144 

145 Ala Pro Pro Gly Tyr His Ala Phe Tyr Cys His Gly Glu Cys Pro Phe 

146 35 40 45 
147 

148 Pro Leu Ala Asp His Leu Asn Ser Thr Asn His Ala lie Val Gin Thr 

149 50 55 60 65 
150 

151 Leu Val Asn Ser Val Asn Ser Lys lie Pro Lys Ala Cys Cys Val Pro 

152 70 75 80 



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RAW SEQUENCE LISTING DATE: Ol/l 1/2002 

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Thr Glu Leu Ser Ala lie Ser Met Leu Tyr Leu Asp Glu Asn Glu Lys 
85 90 95 



Val Val Leu Lys Asn Tyr Gin Asp Met Val Val Glu Gly Cys Gly Cys 
100. 105 110 



Arg 



(2) INFORMATION FOR SEQ ID NO : 3 : 

(i) SEQUENCE CHARACTERISTICS: 

(A) LENGTH: 1607 base pairs 

(B) TYPE: nucleic acid 

(C) STRANDEDNESS : double 

(D) TOPOLOGY: linear 

(ii) MOLECULE TYPE: CDNA to mRNA 



(iii) HYPOTHETICAL: NO 



(iv) ANTI- SENSE: NO 



(vi) ORIGINAL SOURCE: 

(A) ORGANISM: Homo Sapiens 

(G) CELL TYPE: Osteosarcoma Cell Line 

(H) CELL LINE: U-20S 

(vii) IMMEDIATE SOURCE: 

(A) LIBRARY: U20S cDNA in Lambda GTIO 

(B) CLONE: Lambda U20S-3 9 



(viii) POSITION IN GENOME: 
(C) UNITS: bp 

(ix) FEATURE: 

(A) NAME/KEY: CDS 

(B) LOCATION: 356.. 1546 



(ix) FEATURE: 

(A) NAME/KEY: mat_peptide 

(B) LOCATION: 1202.. 1543 



(ix) FEATURE: 

(A) NAME/KEY: mRNA 

(B) LOCATION: 14.. 1607 



(ix) FEATURE: 

(A) NAME/KEY: sig_peptide 

(B) LOCATION: 356.. 424 



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206 

207 (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 3 : 

208 

209 GTCGACTCTA GAGTGTGTGT CAGCACTTGG CTGGGGACTT CTTGAACTTG CAGGGAGAAT 60 
210 

211 AACTTGCGCA CCCCACTTTG CGCCGGTGCC TTTGCCCCAG CGGAGCCTGC TTCGCCATCT 120 
212 

213 CCGAGCCCCA CCGCCCCTCC ACTCCTCGGC CTTGCCCGAC ACTGAGACGC TGTTCCCAGC 180 
214 

215 GTGAAAAGAG AGACTGCGCG GCCGGCACCC GGGAGAAGGA GGAGGCAAAG AAAAGGAACG 24 0 

216 

217 GACATTCGGT CCTTGCGCCA GGTCCTTTGA CCAGAGTTTT TCCATGTGGA CGCTCTTTCA 300 
218 

219 ATGGACGTGT CCCCGCGTGC TTCTTAGACG GACTGCGGTC TCCTAAAGGT CGACC ATG 3 58 

220 Met 

221 -282 
222 

223 GTG GCC GGG ACC CGC TGT CTT CTA GCG TTG CTG CTT CCC CAG GTC CTC 406 

224 Val Ala Gly Thr Arg Cys Leu Leu Ala Leu Leu Leu Pro Gin Val Leu 

225 -280 -275 -270 
226 

227 CTG GGC GGC GCG GCT GGC CTC GTT CCG GAG CTG GGC CGC AGG AAG TTC 454 

228 Leu Gly Gly Ala Ala Gly Leu Val Pro Glu Leu Gly Arg Arg Lys Phe 

229 -265 -260 -255 -250 
230 

231 GCG GCG GCG TCG TCG GGC CGC CCC TCA TCC CAG CCC TCT GAC GAG GTC 502 

232 Ala Ala Ala Ser Ser Gly Arg Pro Ser Ser Gin Pro Ser Asp Glu Val 

233 -245 -240 -235 
234 

23 5 CTG AGC GAG TTC GAG TTG CGG CTG CTC AGC ATG TTC GGC CTG AAA CAG 550 
23 6 Leu Ser Glu Phe Glu Leu Arg Leu Leu Ser Met Phe Gly Leu Lys Gin 
• 237 -230 -225 -220 

238 

23 9 AGA CCC ACC CCC AGC AGG GAC GCC GTG GTG CCC CCC TAC ATG CTA GAC 598 

240 Arg Pro Thr Pro Ser Arg Asp Ala Val Val Pro Pro Tyr Met Leu Asp 

241 -215 -210 -205 
242 

243 CTG TAT CGC AGG CAC TCA GGT CAG CCG GGC TCA CCC GCC CCA GAC CAC 646 

244 Leu Tyr Arg Arg His Ser Gly Gin Pro Gly Ser Pro Ala Pro Asp His 

245 -200 -195 -190 
246 

247 CGG TTG GAG AGG GCA GCC AGC CGA GCC AAC ACT GTG CGC AGC TTC CAC 694 

248 Arg Leu Glu Arg Ala Ala Ser Arg Ala Asn Thr Val Arg Ser Phe His 

249 -185 -180 -175 -170 
250 

251 CAT GAA GAA TCT TTG GAA GAA CTA CCA GAA ACG AGT GGG AAA ACA ACC 742 

252 His Glu Glu Ser Leu Glu Glu Leu Pro Glu Thr Ser Gly Lys Thr Thr 

253 -165 -160 -155 
254 

255 CGG AGA TTC TTC TTT AAT TTA AGT TCT ATC CCC ACG GAG GAG TTT ATC 7 90 

256 Arg Arg Phe Phe Phe Asn Leu Ser Ser lie Pro Thr Glu Glu Phe lie 

257 -150 -145 -140 
258