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-1- 



SEQUENCE LISTING 



<110> Sebbel, Peter 

Dunant, Nicolas 
Bachmann, Martin 
Tissot, Alain 
Lechner , Franziska 

<-120> Molecular Antigen Array 



<130> 1700.0180002 

<140> 
<141> 

<160> 186 

<170> Patentin Ver. 2.1 

<210> 1 
<211> 41 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 1 

ggggacgcgt gcagcaggta accaccgtta aagaaggcac c 41 



<210> 2 
<211> 44 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 2 

cggtggttac ctgctgcacg cgttgcttaa gcgacatgta gcgg 44 

<210> 3 
<211> 20 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 3 

ccatgaggcc tacgataccc 20 



<210> 4 
<211> 25 
<212> DNA 

<213> Artificial Sequence 



<220> 



-2- 



<223> Primer 
<400> 4 

ggcactcacg gcgcgcttta caggc 25 



<210> 5 
<211> 47 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 5 

ccttctttaa cggtggttac ctgctggcaa ccaacgtggt tcatgac 47 



<210> 6 
<211> 40 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 6 

aagcatgctg cacgcgtgtg cggtggtcgg atcgcccggc 40 



<210> 7 
<211> 90 
<212> DNA 

<213> Artificial Sequence 



<220> 

<223> Primer 
<400> 7 

gggtctagat tcccaaccat tcccttatcc aggctttttg acaacgctat gctccgcgcc 60 
catcgtctgc accagctggc ctttgacacc 90 



<210> 8 
<211> 108 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 8 

gggtctagaa ggaggtaaaa aacgatgaaa aagacagcta tcgcgattgc agtggcactg 60 
gctggtttcg ctaccgtagc gcaggccttc ccaaccattc ccttatcc 108 



<210> 9 
<211> 31 
<212> DNA 

<213> Artificial Sequence 



<220> 

<223> Primer 



-3- 

<400> 9 

cccgaattcc tagaagccac agctgccctc c 31 



<210> 10 
<211> 24 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 10 

cctgcggtgg tctgaccgac accc 24 

<210> 11 
<211> 41 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 11 

ccgcggaaga gccaccgcaa ccaccgtgtg ccgccaggat g 41 

<210> 12 
<211> 33 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 12 

ctatcatcta gaatgaatag aggattcttt aac 33 

<210> 13 
<211> 15 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Modified ribosome 
binding site 

<400> 13 

aggaggtaaa aaacg 15 



<210> 14 
<211> 21 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> signal peptide 



<400> 14 

Met Lys Lys Thr Ala lie Ala lie Ala Val Ala Leu Ala Gly Phe Ala 



-4- 



10 



15 



Thr Val Ala Gin Ala 
20 



<210> 15 
<211> 46 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> modified Fos 
construct 

<400> 15 

Cys Gly Gly Leu Thr Asp Thr Leu Gin Ala Glu Thr Asp Gin Val Glu 
15 10 15 

Asp Glu Lys Ser Ala Leu Gin Thr Glu lie Ala Asn Leu Leu Lys Glu 
20 25 30 

Lys Glu Lys Leu Glu Phe lie Leu Ala Ala His Gly Gly Cys 
35 40 45 



<210> 16 
<211> 6 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> peptide linker 
<400> 16 

Ala Ala Ala Ser Gly Gly 
1 5 



<210> 17 . 
<211> 6 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> peptide linker 
<400> 17 

Gly Gly Ser Ala Ala Ala 
1 5 



<210> 18 
<211> 256 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Fos fusion construct 
<400> 18 

gaattcagga ggtaaaaaac gatgaaaaag acagctatcg cgattgcagt ggcactggct 60 
ggtttcgcta ccgtagcgca ggcctgggtg ggggcggccg cttctggtgg ttgcggtggt 120 



-5- 



ctgaccgaca ccctgcaggc ggaaaccgac caggtggaag acgaaaaatc cgcgctgcaa 180 
accgaaatcg cgaacctgct gaaagaaaaa gaaaagctgg agttcatcct ggcggcacac 24 0 
ggtggttgct aagctt 256 



<210> 19 
<211> 52 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Fos fusion construct 
<400> 19 

Ala Ala Ala Ser Gly Gly Cys Gly Gly Leu Thr Asp Thr Leu Gin Ala 
5 10 15 

Glu Thr Asp Gin Val Glu Asp Glu Lys Ser Ala Leu Gin Thr Glu He 
20 25 30 

Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe He Leu Ala Ala 
35 40 45 

His Gly Gly Cys 
50 



<210> 20 
<211> 261 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Fos fusion ^• 
construct 

<220> 

<221> CDS 

<222> (22) . . (240) 

<400> 20 

gaattcagga ggtaaaaaac g atg aaa aag aca get ate gcg att gca gtg 51 

Met Lys Lys Thr Ala He Ala He Ala Val 
15 10 

gca ctg get ggt ttc get ace gta gcg cag gee tgc ggt ggt etg ace 99 
Ala Leu Ala Gly Phe Ala Thr Val Ala Gin Ala Cys Gly Gly Leu Thr 
15 20 25 

gac ace ctg cag gcg gaa ace gae cag gtg gaa gae gaa aaa tec gcg 147 
Asp Thr Leu Gin Ala Glu Thr Asp Gin Val Glu Asp Glu Lys Ser Ala 
30 35 40 

ctg caa ace gaa ate gcg aac etg ctg aaa gaa aaa gaa aag etg gag 195 
Leu Gin Thr Glu He Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu 
45 50 55 

ttc ate etg gcg gca cae ggt ggt tgc ggt ggt tet gcg gee get 240 
Phe He Leu Ala Ala His Gly Gly Cys Gly Gly Ser Ala Ala Ala 
60 65 ■ 70 

gggtgtgggg atatcaaget t 261 



-6- 



<210> 21 
<211> 73 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<400> 21 

Met Lys Lys Thr Ala lie Ala He Ala Val Ala Leu Ala Gly Phe Ala 
1 5 , 10 15 

Thr Val Ala Gin Ala Cys Gly Gly Leu Thr Asp Thr Leu Gin Ala Glu 
20 25 30 

Thr Asp Gin Val Glu Asp Glu Lys Ser Ala Leu Gin Thr Glu He Ala 
35 40 45 

Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe He Leu Ala Ala His 
50 55 60 

Gly Gly Cys Gly Gly Ser Ala Ala Ala 
65 70 ■ 



<210> 22 

<211> 196 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 



□ <220> 

L.L <221> CDS 



<222> (34) . . (189) 
<400> 22 

gaattcagga ggtaaaaaga tatcgggtgt ggg gcg gcc get tct ggt ggt tgc 54 

Ala Ala Ala Ser Gly Gly Cys 
1 5 

ggt ggt ctg acc gac acc ctg cag gcg gaa acc. gac cag gtg gaa gac 102 
Gly Gly Leu Thr Asp Thr Leu Gin Ala Glu Thr Asp cin Val Glu Asp 
10 15 20 

gaa aaa tec gcg ctg caa acc gaa ate gcg aac ctg ctg aaa gaa aaa 150 
Glu Lys Ser Ala Leu Gin Thr Glu He Ala Asn Leu Leu Lys Glu Lys 
25 30 35 

gaa aag ctg gag ttc ate ctg gcg gea cac ggt ggt tgc taagctt 196 
Glu Lys Leu Glu Phe He Leu Ala Ala His Gly Gly Cys 
40 45 50 



<210> 23 
<211> 52 
<212> PRT 

<213> Artificial Sequence 



-7- 



<220> 

<223> Fos fusion 
construct 

<400> 23 

Ala Ala Ala Ser Gly Gly Cys Gly Gly Leu Thr Asp Thr Leu Gin Ala 
15 10 15 

Glu Thr Asp Gin Val Glu Asp Glu Lys Ser Ala Leu Gin Thr Glu He 
20 25 30 

Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe He Leu Ala Ala 
35 40 45 

His Gly Gly Cys 
50 



<210> 24 
<211> 204 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<400> 24 

gaattcagga ggtaaaaaac gatggcttgc 
accgaccagg tggaagacga aaaatccgcg 
gaaaaagaaa agctggagtt catcctggcg 
gctgggtgtg gggatatcaa gctt 



ggtggtctga ccgacaccct gcaggcggaa 60 
ctgcaaaccg aaatcgcgaa cctgctgaaa 120 
gcacacggtg gttgcggtgg ttctgcggcc 180 

204 



<210> 25 
<211> 56 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<400> 25 

Lys Thr Met Ala Cys Gly Gly Leu Thr Asp Thr Leu Gin Ala Glu Thr 

15 10 15 

Asp Gin Val Glu Asp Glu Lys Ser Ala Leu Gin Thr Glu He Ala Asn 

20 25 30 

Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe He Leu Ala Ala His Gly 

35 40 45 

Gly Cys Gly Gly Ser Ala Ala Ala 
50 55 



<210> 26 

<211> 26 

<212> PRT 

<213> Homo sapiens 



<400> 26 

Met Ala Thr Gly Ser Arg Thr Ser Leu Leu Leu Ala Phe Gly Leu Leu 
15 10 15 



Cys Leu Pro Trp Leu Gin Glu Gly Ser Ala 
20 25 



<210> 27 
<211> 262 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<400> 27 

gaattcaggc ctatggctac aggctcccgg 
tgcctgccct ggcttcaaga gggcagcgct 
ggtggtctga ccgacaccct gcaggcggaa 
ctgcaaaccg aaatcgcgaa cctgctgaaa 
gcacacggtg gttgctaagc tt 



acgtccctgc tcctggcttt tggcctgctc 60 
gggtgtgggg cggccgcttc tggtggttgc 120 
accgaccagg tggaagacga aaaatccgcg 180 
gaaaaagaaa agctggagtt catcctggcg 240 

262 



<210> 28 
<211> 52 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<400> 28 

Ala Ala Ala Ser Gly Gly Cys Gly Gly Leu Thr Asp Thr Leu Gin Ala 
5 10 15 

Glu Thr Asp Gin Val Glu Asp Glu Lys Ser Ala Leu Gin Thr Glu He 
20 25 30 

Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe He Leu Ala Ala 
35 40 • 45 . 

His Gly Gly Cys 
50 



<210> 29 
<211> 261 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<220> 

<221> CDS 

<222> (7) . . (240) 

<400> 29 

gaattc atg get aca ggc tec egg acg tec ctg etc etg get ttt ggc 48 

Met Ala Thr Gly Ser Arg Thr Ser Leu Leu Leu Ala Phe Gly 
1 5 10 



-9- 



ctg etc tgc ctg ccc tgg ctt caa gag ggc age get tgc ggt ggt ctg 96 
Leu Leu Cys Leu Pro Trp Leu Gin Glu Gly Ser Ala Cys Gly Gly Leu 
15 20 25 30 

ace gac aec etg cag gcg gaa acc gae eag gtg gaa gac gaa aaa tec 14 4 
Thr Asp Thr Leu Gin Ala Glu Thr Asp Gin Val Glu Asp Glu Lys Ser 
35 40 45 

gcg etg caa aec gaa ate gcg aac ctg ctg aaa gaa aaa gaa aag etg 192 
Ala Leu Gin Thr Glu lie Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu 
50 55 60 

gag ttc ate ctg gcg gca eac ggt ggt tgc ggt ggt tct gcg gee get 240 
Glu Phe He Leu Ala Ala His Gly Gly Cys Gly Gly Ser Ala Ala Ala 
65 70 75 

gggtgtggga ggcetaaget t 261 



<210> 30 
<211> 78 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<400> 30 

Met Ala Thr Gly Ser Arg Thr Ser Leu Leu Leu Ala Phe Gly Leu Leu 
15 10 15 

Cys Leu Pro Trp Leu Gin Glu Gly Ser Ala Cys Gly Gly Leu Thr Asp 
20 25 30 

y Thr Leu Gin Ala Glu Thr Asp Gin Val Glu Asp Glu Lys Ser Ala Leu 

-f: 35 40 45 

O 

Gin Thr Glu He Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe 
50 55 60 

He Leu Ala Ala His Gly Gly Cys Gly Gly Ser Ala Ala Ala 
65 70 75 



<210> 31 
<211> 44 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 31 

eetgggtggg ggeggccgct tctggtggtt geggtggtet gace 44 



<210> 32 

<211> 44 

<212> DNA 

<213> Artificial 



Sequence 



-10- 



<220> 

<223> Primer 
<400> 32 

ggtgggaatt caggaggtaa aaagatatcg ggtgtggggc ggcc 44 



<210> 33 
<211> 47 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<400> 33 

ggtgggaatt caggaggtaa aaaacgatgg cttgcggtgg tctgacc 47 



<210> 34 
<211> 18 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<400> 34 

gcttgcggtg gtctgacc 



<210> 35 
<211> 27 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<400> 35 

ccaccaagct tagcaaccac cgtgtgc 27 



<210> 36 
<211> 54 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 36 

ccaccaagct tgatatcccc acacccagcg gccgcagaac caccgcaacc accg 54 



<210> 37 
<211> 32 
<212> DNA 

<213> Artificial Sequence 



<220> 

<223> Primer 



-11- 



<400> 37 

ccaccaagct taggcctccc acacccagcg gc 32 

<210> 38 
<211> 29 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 38 

ggtgggaatt caggaggtaa aaaacgatg 29 



<210> 39 
<211> 32 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 39 

ggtgggaatt caggcctatg gctacaggct cc 32 



<210> 40 
<211> 27 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 40 

ggtgggaatt' catggctaca ggctccc 27 



<210> 41 
<211> 59 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 41 

gggtctagaa tggctacagg ctcccggacg tccctgctcc tggcttttgg cctgctctg 59 

<210> 42 
<211> 58 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<4G0> 42 

cgcaggcctc ggcactgccc tcttgaagcc agggcaggca gagcaggcca aaagccag 58 



-12- 



<210> 43 
<211> 402 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Modified bee 

venom phospholipase A2 

<220> 

<221> CDS 

<222> (1) . . (402) 

<400> 43 

ate ate tac cca ggt act ctg tgg tgt ggt cac ggc aac aaa tct tct 48 

lie lie Tyr Pro Gly Thr Leu Trp Cys Gly His Gly Asn Lys Ser Ser 
15 10 15 

ggt ccg aac gaa etc ggc cgc ttt aaa cac aec gac gca tgc tgt cgc 96 
Gly Pro Asn Glu Leu Gly Arg Phe Lys His Thr Asp Ala Cys Cys Arg 
20 25 30 

ace cag gac atg tgt ccg gac gtc atg tct get ggt gaa tct aaa cac 144 
Thr Gin Asp Met Cys Pro Asp Val Met Ser. Ala Gly Glu Ser Lys His 
35 40 45 

ggg tta act aac ace get tct cac aeg cgt etc age tgc gac tgc gac 192 
Gly Leu Thr Asn Thr Ala Ser His Thr Arg Leu Ser Cys Asp Cys Asp 
50 55 60 

gac aaa ttc tac gac tgc ctt aag aac tee gee gat ace ate tct tct 240 
Asp Lys Phe Tyr Asp Cys Leu Lys Asn Ser Ala Asp Thr lie Ser Ser 
65 70 75 80 

tac ttc gtt ggt aaa atg tat ttc aac ctg ate gat ace aaa tgt tac 288 
Tyr Phe Val Gly Lys Met Tyr Phe Asn Leu lie Asp Thr Lys Cys Tyr 
85 90 95 

aaa ctg gaa cac ccg gta ace ggc tgc ggc gaa cgt aec gaa ggt cgc 336 
Lys Leu Glu His Pro Val Thr Gly Cys Gly Glu Arg Thr Glu Gly Arg 
100 105 110 

tgc ctg cac tac ace gtt gac aaa tct aaa ccg aaa gtt tac cag tgg 384 
Cys Leu His Tyr Thr Val Asp Lys Ser Lys Pro Lys Val Tyr Gin Trp 
115 120 125 

ttc gac ctg cgc aaa tac 402 
Phe Asp Leu Arg Lys Tyr 
130 



<210> 44 
<211> 134 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Modified bee 

venom phospholipase A2 

<400> 44 

lie lie Tyr Pro Gly Thr Leu Trp Cys Gly His Gly Asn Lys Ser Ser 
15 10 15 



-13- 



Gly Pro Asn Giu Leu Gly Arg Phe Lys His Thr Asp Ala Cys Cys Arg 
20 25 30 

Thr Gin Asp Met Cys Pro Asp Val Met Ser Ala Gly Glu Ser Lys His 
35 40 45 

Gly Leu Thr Asn Thr Ala Ser His Thr Arg Leu Ser Cys Asp Cys Asp 
50 55 60 

Asp Lys Phe Tyr Asp Cys Leu Lys Asn Ser Ala Asp Thr lie Ser Ser 
65. 70 75 80 

Tyr Phe Val Gly Lys Met Tyr Phe Asn Leu lie Asp Thr Lys Cys Tyr 
85 90 95 

Lys Leu Glu His Pro Val Thr Gly Cys Gly Glu Arg Thr Glu Gly Arg 
100 105 110 

Cys Leu His Tyr Thr Val Asp Lys Ser Lys Pro Lys Val Tyr Gin Trp 
115 120 125 

Phe Asp Leu Arg Lys Tyr 
130 



<210> 45 
<211> 19 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 45 

ccatcatcta cccaggtac 19 



<210> 46 
<211> 34 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 46 

cccacaccca gcggccgcgt atttgcgcag gtcg 34 



<210> 47 
<211> 36 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 47 

cggtggttct gcggccgcta tcatctaccc aggtac 36 



<210> 48 



-14- 



<211> 19 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 48 

ttagtatttg cgcaggtcg 19 



<210> 49 
<211> 18 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 49 

ccggctccat cggtgcag 



<210> 50 
<211> 36 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 50 

accaccagaa gcggccgcag gggaaacaca tctgcc 36 



<210> 51 
<211> 35 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 51 

cggtggttct gcggccgctg gctccatcgg tgcag 35 



<210> 52 
<211> 21 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 52 

ttaaggggaa acacatctgc c 21 



<210> 53 
<211> 33 
<212> DNA 

<213> Artificial Sequence 



<220> 

<223> Primer 
<400> 53 

actagtctag aatgagagtg aaggagaaat ate 



<210> 54 
<211> 42 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 54 

tagcatgcta gcaccgaatt tatctaattc caataattct tg 



<210> 55 
<211> 51 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 55 

gtagcaccca ccaaggcaaa gctgaaagct acccagctcg agaaactggc 



<210> 56 
<211> 48 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 56 

caaagctcct attcccactg ccagtttctc gagctgggta gctttcag 



<210> 57 
<211> 36 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 57 

ttcggtgcta gcggtggctg cggtggtctg accgac 



<210> 58 
<211> 37 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



-16- 

<400> 58 

gatgctgggc ccttaaccgc aaccaccgtg tgccgcc 37 



<210> 59 
<211> 46 
<212> PRT 
<213> Artificia, 

<220> 

<223> JUN amino 
sequence 

<400> 59 
Cys Gly Gly Arg 
1 

Ala Gin Asn Ser 
20 

Val Ala Gin Leu 
35 



Sequence 
acid 



lie Ala Arg Leu 
5 

Glu Leu Ala Ser 

Lys Gin Lys Val 
40 



Glu Glu Lys Val 
10 

Thr Ala Asn Met 
25 

Met Asn His Val 



Lys Thr Leu Lys 
15 



Leu Arg Glu Gin 
30 



Gly Cys 
45 



<210> 60 
<211> 46 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> FOS amino 

acid sequence 

<400> 60 

Cys Gly Gly Leu Thr Asp Thr Leu 
1 5 

Asp Glu Lys Ser Ala Leu Gin Thr 
20 

Lys Glu Lys Leu Glu Phe lie Leu 
35 40 



Gin Ala Glu Thr Asp Gin Val Glu 
10 15 

Glu lie Ala Asn Leu Leu Lys Glu 
25 30 

Ala Ala His Gly Gly Cys 
45 



<210> 61 
<211> 33 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 61 

ccggaattca tgtgcggtgg tcggatcgcc egg 33 



<210> 62 

<211> 39 

<212> DNA 

<213> Artificial 



Sequence 



<220> 

<223> Primer 



-17- 



<400> 62 



gtcgctaccc gcggctccgc aaccaacgtg gttcatgac 



39 



<210> 63 
<211> 50 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 63 

gttggttgcg gagccgcggg tagcgacatt gacccttata aagaatttgg 50 



<210> 64 
<211> 38 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<210> 65 
<211> 33 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 65 



<210> 66 
<211> 38 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 66 

cgcgtcccaa gcttttagca accaacgtgg ttcatgac 38 



<210> 67 
<211> 31 
<212> DNA 

<213> Artificial Sequence 



<400> 64 

cgcgtcccaa gcttctacgg aagcgttgat aggatagg 



38 



ctagccgcgg gttgcggtgg tcggatcgcc egg 



33 



<220> 

<223> Primer 



<400> 67 

ccggaattca tggacattga cccttataaa g 



31 



-18- 

<210> 68 
<211> 45 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 68 

ccgaccaccg caacccgcgg ctagcggaag cgttgatagg atagg 45 



<210> 69 
<211> 47 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 69 

ctaatggatc cggtgggggc tgcggtggtc ggatcgcccg gctcgag 47 



<210> 70 
<211> 39 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 70 

gtcgctaccc gcggctccgc aaccaacgtg gttcatgac 39 



<210> 71 
<211> 31 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 71 

ccggaattca tggacattga cccttataaa g 31 



<210> 72 
<211> 48 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 72 

ccgaccaccg cagcccccac cggatccatt agtacccacc caggtagc 48 



<210> 
<211> 
<212> 



73 
45 
DNA 



-19- 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 73 

gttggttgcg gagccgcggg tagcgaccta gtagtcagtt atgtc 45 



<210> 74 
<211> 38 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 74 

cgcgtcccaa gcttctacgg aagcgttgat aggatagg 38 



^. <210> 75 

W <211> 33 

<212> DNA 

H'j <213> Artificial Sequence 

5- <220> 

<223> Primer 

^ <400> 75 

y^ ctagccgcgg gttgcggtgg tcggatcgcc egg 33 

H <210> 76 

Ji- <211> 38 

y <212> DNA 

4^'. <213> Artificial Sequence 

|I. <220> 

<223> Primer 

<400> 76 

cgcgtcccaa gcttttagca accaacgtgg ttcatgac 38 



<210> 77 
<211> 30 
<212> DNA 

<213> 'Artificial Sequence 
<220> 

<223> Primer 
<400> 77 

ccggaattca tggccacact tttaaggagc 30 



•<210> 78 
<211> 38 
<212> DNA 

<213> Artificial Sequence 



<220> 



<223> Primer 
<400> 78 

cgcgtcccaa gcttttagca accaacgtgg ttcatgac 



<210> 79 
<211> 31 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 79 

ccggaattca tggacattga cccttataaa g 



<210> 80 
<211> 51 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 80 

cctagagcca cctttgccac catcttctaa attagtaccc acccaggtag 



<210> 81 
<211> 48 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 81 

gaagatggtg gcaaaggtgg ctctagggac ctagtagtca gttatgtc 



<210> 82 
<211> 38 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 82 

cgcgtcccaa gcttctaaac aacagtagtc tccggaag 



<210> 83 
<211> 36 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<400> 83 



gccgaattcc tagcagctag caccgaattt atctaa 



<210> 84 
<211> 33 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 84 

ggttaagtcg acatgagagt gaaggagaaa tat 



<210> 85 
<211>"30 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 85 

taaccgaatt caggaggtaa aaagatatgg 



<210> 86 
<211> 35 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 86 

gaagtaaagc ttttaaccac cgcaaccacc agaag 



<210> 87 
<211> 33 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 87 

tcgaatgggc cctcatcttc gtgtgctagt cag 



<210> 88 
<211> 4 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<400> 88 
Glu Phe Arg Arg 
1 



-22- 



<210> 89 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 89 

Met Asp lie Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro lie 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Thr Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Gly Ser Gin Cys 
180 



<210> 90 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 90 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro Thr 



-23- 



65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 " 95 

Phe Arg Gin Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Thr Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Cys Val He Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Gly Ser Gin Cys 
180 



<210> 91 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 91 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro He Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 



-24- 



Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 92 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 92 

Met Gin Leu Phe His Leu Cys Leu He lie Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 

20 "25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Asn Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro He Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 . 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 93 

<211> 183 

<212> PRT 

<213> Hepatitis B virus 



<400> 93 
Met Asp lie 
1 

Ser Phe Leu 



Thr Ala Ser 
35 



Asp Pro Tyr Lys 
5 

Pro Thr Asp Phe 
20 

Ala Leu Tyr Arg 



Glu Phe Gly 
10 

Phe Pro Ser 
25 

Glu Ala Leu 
40 



Ala Thr Val Glu Leu Leu 
15 

Val Arg Asp Leu Leu Asp 
30 

Glu Ser Pro Glu His Cys 
45 



Ser Pro His 
50 

Leu Met Thr 
65 



His Thr Ala Leu 
55 

Leu Ala Thr Trp 
70 



Arg Gin Ala 
Val Gly Val 



lie Leu 
60 

Asn Leu 

75 



Cys Trp Gly Glu 



Glu Asp Pro Ala 
80 



Ser Arg Asp 
Phe Arg Gin 



Glu Thr Val 
115 



Leu Val Val Ser 
85 

Leu Leu Trp Phe 
100 

lie Glu Tyr Leu 



Tyr Val Asn 
90 

His lie Ser 
105 

Val Ser Phe 
120 



Thr Asn Met Gly Leu Lys 
95 

Cys Leu Thr Phe Gly Arg 
110 

Gly Val Trp lie Arg Thr 
125 



Pro Pro Ala 
130 



Tyr Arg Pro Pro 
135 



Asn Ala Pro 



lie Leu 
140 



Ser Thr Leu Pro 



Glu Thr Cys 
145 



Val Val Arg Arg 
150 



Arg Gly Arg 



Ser Pro Arg Arg Arg Thr 
155 160 



Pro Ser Pro 



Arg Arg Arg Arg 
165 



Ser Gin Ser 
170 



Pro Arg Arg Arg Arg Ser 
175 



Gin Ser Arg 



Glu Ser Gin Cys 
180 



<210> 94 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 94 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 .25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro Val Ser Arg Asp 



-26- 



100 



105 



110 



Leu Val Val Ser Tyr Val Asn Thr Asn Val Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

lie Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
i45 150 155 160 

Tyr Arg Pro Pro Asn. Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 ' 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 95 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 



<400> 95 

Met Gin Leu Phe His Leu 
1 5 

Val Gin Ala Ser Lys Leu 
20 

Asp Pro Tyr Lys Glu Phe 
35 

Pro Ser Asp Phe Phe Pro 
50 



Cys Leu He lie Ser Cys Ser Cys Pro Thr 
10 15 

Cys Leu Gly Trp Leu Trp Asp Met Asp He 
25 30 

Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
40 45 

Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
55 60 



Ala Leu Tyr Arg 
65 

His Thr Ala Leu 



Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 

70 75 80 

Arg Gin Ala He Leu Cys Trp Gly Asp Leu Met Thr 

85 90 95 



Leu Ala Thr Trp 
100 

Leu Val Val Ser 
115 

Leu Leu Trp Phe 
130 

He Glu Tyr Leu 
145 

Tyr Arg Pro Pro 



Val Gly Gly Asn Leu Glu Asp Pro Val Ser Arg Asp 
105 110 

Tyr Val Asn Thr Asn Val Gly Leu Lys Phe Arg Gin 
120 125 

His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
135 140 

Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
150 155 160 

Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 



-27- 



Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 96 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 96 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro Gin 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly' Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro He Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 ^ 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 97 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 



-28- 



<400> 97 
Met Gin Leu Phe 
1 



Val Gin Ala Ser 
20 



Asp Pro Tyr Lys 
35 

Pro Ser Asp Phe 
50 



Ala Leu Tyr Arg 
65 



His Thr Ala Leu 



His Leu Cys Leu 
5 

Lys Leu Cys Leu 



Glu Phe Gly Ala 
40 

Phe Pro Ser Val 
55 

Glu Ala Leu Glu 
70 

Arg Gin Ala He 
85 



He He Ser Cys 
10 

Gly Trp Leu Trp 
25 

Thr Val Glu Leu 



Arg Asp Leu Leu 
60 

Ser Pro Glu His 
75 

Leu Cys Trp Gly 
90 



Ser Cys Pro Thr 
15 

Gly Met Asp He 
30 

Leu Ser Phe Leu 
45 

Asp Thr Ala Ser 



Cys Ser Pro His 
80 

Glu Leu Met Thr 
95 



Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Lys Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Gly Ser Gin Cys 
210 



<210> 98 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 98 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Phe Arg Asp Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro Ala 



# 



-29- 

65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Giy Arg 
100 105 110 

Asp Thr Val lie Giu Tyr Leu Vai Ser Phe Giy Val Trp lie Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Ser Asn Ala Pro lie Leu Ser Thr Leu Pro 
130 135 140 

Giu Thr Cys Vai Vai Arg Arg Arg Giy Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Giu Ser Gin Cys 
180 



<210> 99 

<211> 183 ■ 
<212> PRT 

<213> Hepatitis B virus 
<400> 99 

Met Asp lie Asp Pro Tyr Lys Giu Phe Giy Ala Thr Vai Giu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Vai Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Giu Ala Leu Giu Ser Pro Giu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Aia lie Leu Cys Trp Giy Giu 
50 55 ' 60 

Leu Met Thr Leu Aia Thr Trp Vai Giy Vai Asn Leu Giu Asp Pro Aia 
65 70 75 80 

Ser Arg Asp Leu Vai Vai Ser Tyr Vai Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Giy Arg 
100 105 110 

Giu Thr Val lie Giu Tyr Leu Vai Ser Phe Giy Vai Trp lie Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Aia Pro lie Leu Ser Thr Leu Pro 
130 135 140 

Giu Thr Thr Vai Vai Arg Arg Arg Giy Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 



-30- 



Gln Ser Arg Glu Ser Gin Cys 
180 



<210> 100 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 100 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 • 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

CJ; Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 

65 70 75 80 

5" His Thr Ala Leu Arg His Ala He Leu Cys Trp Gly Asp Leu Arg Thr 

X 85 ■ 90 95 

Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro He Ser Arg Asp 
N= 100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

^ Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 

O 130 135 140 

£' 

iH He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 

145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185. 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205, 

Glu Ser Gin Cys 
210 



<210> 101 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 101 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
1 5 10 . 15 



3 1 



Val Gin Ala Ser 



Lys Leu Cys Leu Gly Trp Leu Trp Asp Met 



Asp He 



-31- 



20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Phe Arg Asp Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 



Leu Ala Thr Trp Val 
100 

Leu Val Val Ser Tyr 
115 

Leu Leu Trp Phe His 
130 

He Glu Tyr Leu Val 
145 

Tyr Arg Pro Pro Asn 
165 

Val Val Arg Arg Arg 
180 

Arg Arg Arg Arg Ser 
195 



Gly Ala Asn Leu Glu Asp 
105 

Val Asn Thr Asn Met -Gly 
120 

He Ser Cys Leu Thr Phe 
135 

Ser Phe Gly Val Trp lie 
150 155 

Ala Pro He Leu Ser Thr 
170 

Gly Arg Ser Pro Arg Arg 
185 

Gin Ser Pro Arg Arg Arg 
200 



Pro Ala Ser Arg Asp 
110 

Leu Lys Phe Arg Gin 
125 

Gly Arg Glu Thr Val 
140 

Arg Thr Pro Gin Ala 
160 

Leu Pro Glu Thr Cys 
175 

Arg Thr Pro Ser Pro 
190 

Arg Ser Gin Ser Arg 
205 



Glu Ser Gin Cys 
210 



<210> 102 
<211> 183 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> synthetic 

human Hepatitus B construct 

.<400> 102 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu 
50 55 - 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala 
65 70 75 80 



-32- 



Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Thr Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 103 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 103 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 = 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp Leu Met Ser 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro He Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 



-33- 



Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 104 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 104 

Met Asp lie Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 ■ 25 30 

Thr Ala Ser Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Thr Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 105 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 105 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 



-34- 



Ser Pro 
50 

Leu Met 
65 



35 

His His Thr Ala 



40 

Leu Arg 
55 



Gin Ala 



Thr Leu 



Ser Arg Asp Leu 
Phe Arg 



Ala Thr 
70 

Val Val 
85 



Trp Val Gly Val 
Ser Tyr 



lie Leu 
60 

Asn Leu 
75 



45 



Cys Trp Gly Asp 



Glu Asp Pro Ala 
80 



Val Asn 
90 



Thr Asn Met Gly Leu Lys 
95 



Glu Thr 



Gin Leu 
100 

Val He 
115 



Pro Pro 
130 



Ala Tyr 
Thr Val 
Pro Ser Pro Arg 



Leu Trp Phe His 
Glu Tyr 
Arg Pro 



He Ser 
105 



Leu Val 
120 



Cys Leu 
Ser Phe Gly Val 



Pro Asn 
135 



Ala Pro 



Glu Thr 
145 



Val Arg 
150 



Gin Ser 



Arg Glu 
180 



Arg Arg Gly Arg 
Arg Ser 
Ser Gin Cys 



He Leu 
140 

Ser Pro 
155 



Thr Phe Gly Arg 
110 

Trp He Arg Thr 
125 

Ser Thr Leu Pro 



Arg Arg Arg Thr 
160 



Arg Arg 
165 



Gin Ser 
170 



Pro Arg Arg Arg Arg Ser 
• 175 



<210> 106 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 106 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 

20 ' 25 30 

Thr Ala Ser Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Ala Asn Leu Glu Asp Pro Ala 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His He Ser Cys ^ Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 



Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 



-35- 



Glu Thr Thr Val Val Arg Arg Arg Gly Arg Thr Pro Arg Arg Arg Thr 
145 150 . 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 107 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 107 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
1 5 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp'ciy Met Asp He 
20 -.25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 108 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 



-36- 



<400> 108 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 109 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 109 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Thr Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 ■ 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 



-37- 



65 

His Thr Ala Leu 



Leu Ala 



Leu Val 



Thr Trp 
100 

Val Ser 
115 



70 

Arg Gin Ala 
85 

Val Gly Val 
Tyr Val Asn 



75 

lie Leu Cys Trp Gly Glu 
90 



Asn Leu 
105 

Thr Asn 
120 



Glu Asp Pro Ala 



Met Gly Leu Lys 
125 



80 

Leu Met Thr 
95 

Ser Arg Asp 
110 

Phe Arg Gin 



Leu Leu 
130 

lie Glu 
145 



Trp Phe 
Tyr Leu 



Tyr Arg Pro Pro 



His lie Ser 
135 

Val Ala Phe 
150 

Asn Ala Pro 
165 



Cys Leu Thr Phe Gly Arg 
140 

Gly Val Trp lie Arg Thr 
155 

lie Leu Ser Thr Leu Pro 
170 



Glu Thr Val 



Pro Pro Ala 
160 

Glu Thr Thr 
175 



Val Val 



Arg Arg 



Arg Arg 
180 

Arg Arg 
195 



Arg Gly Arg 



Ser Gin Ser 



Ser Pro 
185 

Pro Arg 
200 



Arg Arg Arg Thr 



Arg Arg Arg Ser 
205 



Pro Ser Pro 
190 

Gin Ser Arg 



Glu Ser 
210 



Gin Cys 



<210> 110 

<211> 212 . : 

<212> PRT 

<213> Hepatitis B virus 
<400> 110 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Phe Glu Cys Ser Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro He Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 



-38- 



Ile Glu Tyr Leu Val Ser Phe Gly 
145 150 

Tyr Arg Pro Pro Asn Ala Pro lie 
165 

Val Val Arg Arg Arg Gly Arg Ser 
180 

Arg Arg Arg Arg Ser Gin Ser Pro 
195 200 

Glu Ser Gin Cys 
210 



Val Trp He Arg Thr Pro Pro Ala 
155 160 

Leu Ser Thr Leu Pro Glu Thr Thr 
170 175 

Pro Arg Arg Arg Thr Pro Ser Pro 
185 190 

Arg Arg Arg Arg Ser Gin Ser Arg 
205 



<210> 111 
<211> 212 
<21-2> PRT 

<213> Hepatitis B virus 
<220> 

<221> UNSURE 

<222> (28).. (28) 

<223> May be any amino acid. 



<400> 111 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Xaa Asp Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp Leu He Thr 
85 90 95 

Leu Ser Thr Trp Val Gly Gly Asn Leu Glu Asp Pro Thr Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 



Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 



Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Thr Gin Ser Arg 
195 200 205 



Glu Ser Gin Cys 
210 



<210> 112 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 112 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Asn Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 113 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 



<400> 113 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 



-40- 



1 5 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 ^ 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His lie Cys Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 • 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys" 
210 



<210> 114 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 114 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 



-41- 



His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 



Glu Pro Gin Cys 
210 



<210> 115 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 115 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
1 5 . 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp ' Phe Phe Pro Ser Val Arg Asp Leu Leu Ser Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 



-42- 



Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 116 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 116 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Leu Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 , 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 117 



-43- 



<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 117 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Asp Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Lys Gin 
115 120 125 

Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 118 
<211> 212 
<212> PRT 
<213> Hepatitis 

<400> 118 
Met Gin Leu Phe 
1 

Val Gin Ala Ser 
20 

Asp Pro Tyr Lys 
35 



B virus 

His Leu Cys Leu 
5 

Lys Leu Cys Leu 

Glu Phe Gly Ala 
40 



He He Ser Cys 
10 



Gly Trp Leu Trp 
25 

Thr Val Glu Leu 



Ser Cys Pro Thr 
15 

Gly Met Asp He 
30 

Leu Ser Phe Leu 
45 



-44- 



Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ala 
50 55 60 

Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Thr Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

Leu Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 119 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 119 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Ser Met Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Tyr Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Thr Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Gly Asn Leu Gin Asp Pro Thr 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His Val Ser Cys Leu Thr Phe Gly Arg 
.100 105 110 

Glu Thr Val Val Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 



Pro Gin Ala Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Cys Val Vai Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 120 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 120 

Met Asp lie Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg His Val Phe Leu Cys Trp Gly Asp 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro Thr 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Thr Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Glu Ser Gin- Cys 
180 



<210> 121 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 



<400> 121 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 



-46- 



10 



15 



Val Gin Ala Ser Lys Leu- Cys Leu Gly 
20 .25 



Trp Leu Trp Gly Met Asp lie 
30 



Asp Pro Tyr Lys Glu Phe Gly Ala Thr 
35 40 



Val Glu Leu Leu Ser Phe Leu 
45 



Pro Ser Asp Phe Phe Pro Ser Val Arg 
50 55 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser 
65 70 



Asp Leu Leu Asp Thr Ala Ser 
60 

Pro Glu His Cys Ser Pro His 
75 80 



His Thr Ala Leu 



Arg Gin Ala lie Leu 
85 



Cys Trp Gly Asp Leu Thr Thr 
90 95 



Leu Ala 



Leu Val 



Leu Leu 
130 

He Glu 
145 



Thr Trp 
100 

Val Ser 
115 

Trp Phe 
Tyr Leu 



Tyr Arg Pro Pro 



Val Gly Val Asn Leu 
105 

Tyr Val Asn Thr Asn 
120 

His lie Ser Cys Leu 
135 

Val Ser Phe Gly Val 
150 

Asn Ala Pro He Leu 
165 



Glu Asp Pro Ala Ser Arg Asp 
110 

Met Gly Leu Lys Phe Arg Gin 
125 

Thr Phe Gly Arg Glu Thr Val 
140 

Trp He Arg Thr Pro Pro Ala 
155 160 

Ser Thr Leu Pro Glu Thr Thr 
170 175 



Val Val Arg Arg Arg Gly Arg Ser Pro 
180 185 

Arg Arg Arg Arg Ser Gin Ser Pro Arg 
195 200 



Arg Arg Arg Thr Pro Ser Pro 
190 

Arg Arg Arg Ser Gin Ser Arg 
205 



Glu Ser 
210 



Gin Cys 



<210> 122 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 122 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 



-47- 



His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu He Phe Gly Arg Glu Thr Val 
130 135 . 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 . 205 

Glu Ser Gin Cys 
210' 



<210> 123 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 123 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
1 5 "10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp 
50 55 • 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Val 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Val Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Thr Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 



Pro Ser Pro Ala Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 



Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 124 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 124 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 ■ 25 • 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65. 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp Leu Met Asn 
85 90 95 

Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro Val Ser Arg Asp 
100 105 110 

Leu Val Val Gly Tyr Val Asn Thr Thr Val Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 125 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 



<400> 125 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 



-49- 



15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
■ 20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Asp 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val He Glu Tyr Leu Val Ser Phe Gly Val Trp lie Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Thr Val Val Arg Arg Arg Gly Arg Thr Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 126 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 126 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
1 5 10 15 ' 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Ala Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 



-50- 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

He Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 127 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 127 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
1 5 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Thr Arq Asp 
100 105 no 

Leu Val Val Ser Tyr Val Asn Thr Asn Val Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 



-51- 



Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 128 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<4.00> 128 

Met Gin Leu Phe His Leu Cys Leu He lie Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Arg He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 . 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Thr Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 129 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 



<400> 129 

Met Gin Leu Phe His Leu Cys 



Leu Val He Ser Cys Ser Cys Pro Thr 



-52- 



10 



15 



Val Gin Ala Ser 
20 



Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 
25 30 



Asp Pro Tyr Lys Glu Phe Gly Ala 
35 40 



Thr Val Glu Leu Leu Ser Phe Leu 
45 



Pro Ser Asp Phe Phe Pro Ser Val 
50 55 



Arg Asp Leu Leu Asp Thr Ala Ala 
60 



Ala Leu Tyr Arg 
65 



Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
70 75 80 



His Thr Ala Leu 



Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 



Leu Ala Thr Trp Val Gly 
100 

Leu Val Val Asn Tyr Val 
115 



Asn Asn Leu Glu Asp Pro Ala Ser Arg Asp 
105 110 

Asn Thr Asn Met Gly Leu Lys He Arg Gin 
120 125 



Leu Leu Trp Phe His He Ser Cys 
130 135 



Leu Thr Phe Gly Arg Glu Thr Val 
140 



Leu Glu Tyr Leu Val Ser 
145 150 



Phe Gly Val Trp He Arg Thr Pro Pro Ala 
155 160 



Tyr Arg Pro Pro 



Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 



Val Val Arg Arg Arg Gly 
180 

Arg Arg Arg Arg Ser Gin 
195 



Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 

185 190 

Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 

200 205 



Glu Ser Gin Cys 
210 



<210> 130 
<211> 212 
<212> PRT 
<213> Hepatitis 

<400> 130 
Met Gin Leu Phe 
1 

Val Gin Ala Ser 
20 

Asp Pro Tyr Lys 
35 

Pro Ser Ala Phe 
50 

Ala Leu Tyr Arg 
65 



B virus 

His Leu Cys Leu 
5 

Lys Leu Cys Leu 

Glu Phe Gly Ala 
40 

Phe Pro Ser Val 
55 

Glu Ala Leu Glu 
70 



He He Ser Cys 
10 

Gly Trp Leu Trp 
25 

Thr Val Glu Leu 



Arg Asp Leu Leu 
60 



Ser Pro Glu His 
75 



Ser Cys Pro Thr 
15 

Gly Met Asp He 
30 

Leu Ser Phe Leu 
45 

Asp Thr Ala Ser 



Cys Ser Pro His 
80 



His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 131 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 131 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ala Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu' Arg Gin Ala He Leu Cys Trp Gly Glu 
50 55 60 

Leu Met Thr Leu Ala Thr 'Trp Val Gly Asn Asn Leu Glu Asp Pro Ala 
65 70 75 80 

Ser Arg Asp Leu Val Val Asn Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95- 

He Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 " 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 



Glu Thr Thr Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 



-54- 



Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 132 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 132 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
1 5 • 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro He 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90^ 95 

Phe Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Cys Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Gly Ser Gin Cys 
180 

<210> 133 
<211> 3221 
<212> DNA 

<213> Hepatitis B virus 

<220> 
<221> CDS 

<222> (1901) . . (2458) 
<400> 133 

ttccactgcc ttccaccaag ctctgcagga ccccagagtc aggggtctgt attttcctgc 60 



tggtggctcc agttcaggaa cagtaaaccc tgctccgaat attgcctctc acatctcgtc 120 



aaLCtCCycg 


dygdcuyyyy 


dOL-L-Ly LydL- 




v^uL-y uy LLdL- 


dyyL-yyyy l l 


rr t"' ^ n ^ n ^ "i" 3 

yudyay LOLd 


yciL-Loy '-yy ^ 


yyd^L. L.v^i.v..>L. 


Lyyi^UdddciL 


LoyLrdy n-'Ov.^ 


L>ddL>L' LL'U-dd 


LU^^Lyy l Ldu 


LryoLyyd uy l 


y uL-LyL-yyoy 


dCyCCLCdLC 


Ll_CLCdLLyy 


L LL-L LOLyyd 




ccaacaacdd 


L-Udy LdL-yyy 


aggcaact ct 


aliyLLLCCOL 


odLy LLyL-Ly 


tattcccatc 


ccatcgtcct 


ft f-f ft f~^ •i~ ■i — H ft 

gggctLLcgc 


LLLCLCLUyy 


CTI.CdyUUCa.C 


Ldy LyL-OdL L 


uyi_LLyyCLL 


H^ayOLd La L 


yydLydLy Ly 


ydyLCOOl-LL 


dLdL-x^yt^Ly L 


Ld^Odd L L L L 


aacaaaacaa 


aaagatgggg 


LUdLLCOOLd 


ygaacai- Lgc 


CaCdygaLCci 


LdLLy Udt^dd 


gttaacaggc 


4~ 4~ /T 4~ "f~ 

CLaLLgaLLy 


gadagLciLy L 


gctccat tta 


cacaatgtgg 


aLdLCOl^gL-O 


aaacaggctt 


LCaCllLLCLC 


gCCddOLLdO 


"f~ /~i /-I 

CT!.LL.aCCCCg 


L LyuLuyyt^d 


dL-yyoL-Lyy l 


dOLyy Luyyy 


yL-L Lyyood l 


dyy^oa uv^ciy 


CCgdLCCa La 


c Lgcggaac L 


UULdyooyL-L 


ctcat cggaa 


ctgacaaLLC 


Ly LOy LL-ULU 


ctaggctgta 


ctgccaactg 


gaLCCLCL*gL. 


ctgaat cccg 


cggacgaccc 


+• r^^-f^ftftftftr^ 

ULt-Luyyyyo 


ctgccgttcc 


agccgaccac 


yyyyoyudL-L* 


LCLCduccgc 


cggT-ccgLgL 


yudL. L LOyL- L 


tgaacgccca 


tcagatcctg 


cccaaggtct 


tgtcaacgac 


cgaccttgag 


gcctacttca 


tgggggagga 


gattaggtta 


aaggtctttg 


gcgcaccagc 


accatgcaac 


tttttcacct 


tgttcaagcc 


tccaagctgt 


gccttgggtg 



tat aaa gaa ttt gga get act gtg 



-55- 



aaaca tcrcrao 

^ c;^ \^ u, ^ u ^ 


aacatcaca t 


caggatt cct 


180 


tttattgttg 


acaagaatcc 


tcacaatacc 


240 


caattttata 


gggggatcac 


ccgtgtgtct 


300 


tcactcacca 


acctcctgtc 


ctccaatttg 


360 


ttttatcata 


ttcctcttca 


tcctgctgct 


420 


ttatcaaggt 


atgttgcccg 


tttgtcctct 


480 


accatgcaaa 


acctgcacga 


ctcctgctca 


540 


t acaaaacct 


a egg tt gga a 


attgcacctg 


600 


aaaataccta 


taaaaataaa 
'-yyy^-^y '-yyy 


cct cagt ccg 


660 


t"ni~i~r'r3CTtcra 
^ L- L. w y ^ y y 


ttcgtagggc 


tttcccccac 


720 


atattQcrocra 


ccaagtctgt 


acagcatcgt 


780 


cttttgtctc 


tgggtataca 


tttaaaccct 


840 


aacttcatgg 


gttacataat 


tggaagt tgg 


900 


aaga t caaac 


actgttt tag 


aaaactt cct 


960 


caaagaat tg 


tgggtctttt 


gggctttgct 


1020 


ttaatgcctt 


tgtatgcatg 


tatacaggct 


1080 


aaaacct ttc 


taagtaaaca 


gtacatgaac 


1140 


ctgtgccaag 


tgtttgctga 


cgcaaccccc 


1200 


cgcatgagtg 


gaacct t tgt 


ggctcctctg 


1260 


tgtattgctc 


gcagccggtc 


tggagcaaag 


1320 


tcgcggaaat 


atacat cgtt 


t ccatggctg 


1380 


gggacgt cct 


ttgtttacgt 


cccgtcggcg 


1440 


cact tcrcraac 


t ctatcat cc 


ccttctccgt 


1500 


tctctttacg 


cggtct cccc 


gtctgtgcct 


1560 


tcacct ctgc 


acgt tgcatg 


gaga cca ccg 


1620 


tacataagag 


gactcttgga 


ctcccagcaa 


1680 


aagactgtgt 


gtttaaggac 


tgggaggagc 


1740 


tattaggagg 


ctgtaggcat 


aaattggtct 


1800 


ctgcctaatc 


atctcttgta 


catgtcccac 


1860 


gctttggggc 


atg gac att gac cct 
Met Asp lie Asp Pro 


1915 



1 5 
tta etc teg ttt ttg cct tet 1963 



-56- 



Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu Pro Ser 
10 15 20 

gac ttc ttt cct tec gtc aga gat etc eta gae acc gee tea get ctg 2011 
Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser Ala Leu 
25 30 35 

tat ega gaa gee tta gag tct ect gag eat tgc tea eet cac cat act 2059 
Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His His Thr 
40 45 50 

gea etc agg caa gee att etc tgc tgg ggg gaa ttg atg act eta get 2107 
Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr Leu Ala 
55 60 65 

acc tgg gtg ggt aat aat ttg gaa gat cca gea tec agg gat eta gta 2155 
Thr Trp Val Gly Asn Asn Leu Glu Asp Pro Ala Ser Arg Asp Leu Val 
70 75 80 85 

gte aat tat gtt aat act aac atg ggt tta aag ate agg caa eta ttg 2203 
Val Asn Tyr Val Asn Thr Asn Met Gly Leu Lys He Arg Gin Leu Leu 
90 95 100 

tgg ttt eat ata tct tgc ctt act ttt gga aga gag act gta ctt gaa 2251 
Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val Leu Glu 
105 110 115 

tat ttg gte tct ttc gga gtg tgg att cgc act ect cca gee tat aga 2299 
Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala Tyr Arg 
120 125 . 130 

cca cca aat gee eet ate tta tea aca ctt eeg gaa act act gtt gtt 2347 
Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr Val Val 
135 140 145 

aga cga egg gac cga ggc agg tec ect aga aga aga act ccc teg cct 2395 
Arg Arg Arg Asp Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
150 155 160 165 

cgc aga cgc aga tct caa teg ccg cgt cgc aga aga tct caa tct egg 244 3 
Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
170 175 180 

gaa tct caa tgt tag tattccttgg actcataagg tgggaaactt tactgggett 2498 
Glu Ser Gin Cys 
185 



tattcctcta 


cagtaectat 


ctttaatect 


gaatggcaaa 


ctecttcctt 


tectaagatt 


2558 


eatttacaag 


aggacattat 


tgataggtgt 


caacaatttg 


tgggccctet 


eaetgtaaat 


2618 


gaaaagagaa 


gattgaaatt 


aattatgcet 


gctagattct 


ateetaceca 


cactaaatat 


2678 


ttgeccttag 


acaaaggaat 


taaaccttat 


tateeagate 


aggtagttaa 


tcattaette 


2738 


caaaecagac 


attatttaea 


tactetttgg 


aaggctggta 


ttetatataa 


gagggaaacc 


2798 


aeacgtageg 


cateattttg 


cgggteacca 


tattettggg 


aacaagaget 


acageatggg 


2858 


aggttggtca 


ttaaaacete 


gcaaaggcat 


ggggaegaat 


etttetgttc 


ceaaccctct 


2918 


gggattcttt 


cecgatcate 


agttggaeee 


tgcattegga 


geeaaetcaa 


acaatecaga 


2978 



-57- 

ttgggacttc aaccccatca aggaccactg gccagcagcc aaccaggtag gagtgggagc 3038 
attcgggcca gggctcaccc ctccacacgg cggtattttg gggtggagcc ctcaggctca 3098 
gggcatattg accacagtgt caacaattcc tcctcctgcc tccaccaatc ggcagtcagg 3158 
aaggcagcct actcccatct ctccacctct aagagacagt catcctcagg ccatgcagtg 3218 
gaa 3221 



<210> 134 
<211> 185 
<212> PRT 

<213> Hepatitis B virus 
<400> 134 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 

15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 

20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 

35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu 

50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Asn Asn Leu Glu Asp Pro Ala 
65 70 75 80 

Ser Arg Asp Leu Val Val Asn Tyr Val Asn Thr Asn Met Gly Leu Lys 

85 90 95 

He Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg 

100 105 110 

Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 

115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 

130 135 140 

Glu. Thr Thr Val Val Arg Arg Arg Asp Arg Gly Arg Ser Pro Arg Arg 
145 150 155 160 

Arg Thr Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg 

165 170 175 

Arg Ser Gin Ser Arg Glu Ser Gin Cys 
180 185 



<210> 135 
<211> 188 
<212> PRT 

<213> Woodchuck hepatitis B virus 
<400> 135 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ser Ser Tyr Gin Leu Leu 
15 10 15 

Asn Phe Leu Pro Leu Asp Phe Phe Pro Asp Leu Asn Ala Leu Val Asp 
20 25 30 

Thr Ala Thr Ala Leu Tyr Glu Glu Glu Leu Thr Gly Arg Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala He Arg Gin Ala Leu Val Cys Trp Asp Glu 
50 55 60 

Leu Thr Lys Leu He Ala Trp Met Ser Ser Asn He Thr Ser Glu Gin 



-58- 



65 70 75 80 

Val Arg Thr He He Val Asn His Val Asn Asp Thr Trp Gly Leu Lys 
85 90 . 95 

Val Arg Gin Ser Leu Trp Phe His Leu Ser Cys Leu Thr Phe Gly Gin 
100 ' 105 110 

His Thr Val Gin Glu Phe Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

Pro Ala Pro Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

Glu His Thr Val He Arg Arg Arg Gly Gly Ala Arg Ala Ser Arg Ser 
145 150 155 160. 

Pro Arg Arg Arg Thr Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro 
165 170 175 

Arg Arg Arg Arg Ser Gin Ser Pro Ser Thr Asn Cys 
180 * 185 



<210> 136 
<211> 217 
<212> PRT 

<213> Ground squirrel hepatitis virus 
<400> 136 

Met Tyr Leu Phe His Leu Cys Leu Val Phe Ala Cys Val Pro Cys Pro 
1 5 - 10 15 

Thr Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Asp Met Asp 
20 25 30 

He Asp Pro Tyr Lys Glu Phe Gly Ser Ser Tyr Gin Leu Leu Asn Phe 
35 40 45 

Leu Pro Leu Asp Phe Phe Pro Asp Leu Asn Ala Leu Val Asp Thr Ala 
50 55 60 

Ala Ala Leu Tyr Glu Glu Glu Leu Thr Gly Arg Glu His Cys Ser Pro 
65 70 75 80 

His His Thr Ala He Arg Gin Ala Leu Val Cys Trp Glu Glu Leu Thr 
85 90 95 

Arg Leu He Thr Trp Met Ser Glu Asn Thr Thr Glu Glu Val Arg Arg 
100 105 110 

He He Val Asp His Val Asn Asn Thr Trp Gly Leu Lys Val Arg Gin 
115 120 125 

Thr Leu Trp Phe His Leu Ser Cys Leu Thr Phe Gly Gin His Thr Val 
130 135 140 

Gin Glu Phe Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Ala Pro 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu His Thr 
165 170 175 



-59- 



Val He Arg Arg Arg Gly Gly Ser Arg Ala Ala Arg Ser Pro Arg Arg 
180 185 190 

Arg Thr Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg 
195 200 205 

Arg Ser Gin Ser Pro Ala Ser Asn Cys 
210 215 



<210> 137 
<211> 262 
<212> PRT 

<213> Snow Goose Hepatitis B Virus 
<400> 137 

Met Asp Val Asn Ala Ser Arg Ala Leu Ala Asn Val Tyr Asp Leu Pro 
1 5 ' 10 15 

Asp Asp Phe Phe Pro Lys He Glu Asp Leu Val Arg Asp Ala Lys Asp 
20 25 30 

Ala Leu Glu Pro Tyr Trp Lys Ser Asp Ser He Lys Lys His Val Leu 
35 40 45 

He Ala Thr .His Phe Val Asp Leu He Glu Asp Phe Trp Gin Thr Thr 
50 55 60 

Gin Gly Met His Glu He Ala Glu Ala He Arg Ala Val He Pro Pro 
65 70 75 80 

Thr Thr Ala Pro Val Pro Ser Gly Tyr Leu He Gin His Asp Glu Ala 
85 90 95 

Glu Glu He Pro Leu Gly Asp Leu Phe Lys Glu Gin Glu Glu Arg He 
100 105 110 

Val Ser Phe Gin Pro Asp Tyr Pro He Thr Ala Arg He His Ala His 
115 120 125 

Leu Lys Ala Tyr Ala Lys He Asn Glu Glu Ser Leu Asp Arg Ala Arg 
130 135 140 

Arg Leu Leu Trp Trp His Tyr Asn Cys Leu Leu Trp Gly Glu Ala Thr 
145 150. 155 160 

Val Thr Asn Tyr He Ser Arg Leu Arg Thr Trp Leu Ser Thr Pro Glu 
165 170 175 

Lys Tyr Arg Gly Arg Asp Ala Pro Thr- He Glu Ala He Thr Arg Pro 
180 185 190 

He Gin Val Ala Gin Gly Gly Arg Lys Thr Ser Thr Ala Thr Arg Lys 
195 200 205 

Pro Arg Gly Leu Glu Pro Arg Arg Arg Lys Val Lys Thr Thr Val Val 
210 215 220 

Tyr Gly Arg Arg Arg Ser Lys Ser Arg Glu Arg Arg Ala Ser Ser Pro 
225 230 235 240 



Gin Arg Ala Gly Ser Pro Leu Pro Arg Ser Ser Ser Ser His His Arg 
245 250 255 



-50- 



Ser Pro Ser Pro Arg Lys 
260 



<210> 138 
<211> 305 
<212> PRT 

<213> Duck hepatitis B virus 
<400> 138 

Met Trp Asp Leu Arg Leu His Pro Ser Pro Phe Gly Ala Ala Cys Gin 
1 .5 10 . 15 

Gly lie Phe Thr Ser Ser Leu Leu Leu Phe Leu Val Thr Val Pro Leu 
20 25 . 30 

Val Cys Thr lie Val Tyr Asp Ser Cys Leu Cys Met Asp lie Asn Ala 
35 40 45 

Ser Arg Ala Leu Ala Asn Val Tyr Asp Leu Pro Asp Asp Phe Phe Pro 
50 55 60 

Lys lie Asp Asp Leu Val Arg Asp Ala Lys Asp Ala Leu Glu Pro Tyr 
65 70 75 80 

Trp Arg Asn Asp Ser lie Lys Lys His Val Leu lie Ala Thr His Phe 
85 90 95 

Val Asp Leu He Glu Asp Phe Trp Gin Thr Thr Gin Gly Met His Glu 
100 105 110 

He Ala Glu Ala Leu Arg Ala He He Pro Ala Thr Thr Ala Pro Val 
115 120 125 

Pro Gin Gly Phe Leu Val Gin His Glu Glu Ala Glu Glu He Pro Leu 
130 135 140 

Gly Glu Leu Phe Arg Tyr Gin Glu Glu Arg Leu Thr Asn Phe Gin Pro 
145 150 155 160 

Asp Tyr Pro Val Thr Ala Arg He His Ala His Leu Lys Ala Tyr Ala 
165 170 175 

Lys He Asn Glu Glu Ser Leu Asp Arg Ala Arg Arg Leu Leu Trp Trp 
180 185 190 

His Tyr Asn Cys Leu Leu Trp Gly Glu Pro Asn Val Thr Asn Tyr He 
195 200 205 

Ser Arg Leu Arg Thr Trp Leu Ser Thr Pro Glu Lys Tyr Arg Gly Lys 
210 215 220 

Asp Ala Pro Thr He Glu Ala He Thr Arg Pro He Gin Val Ala Gin 
225 230 235 240 

Gly Gly Arg Asn Lys Thr Gin Gly Val Arg Lys Ser Arg Gly Leu Glu 
245 250 255 

Pro Arg Arg Arg Arg Val Lys Thr Thr He Val Tyr Gly Arg Arg Arg 
260 265 270 

Ser Lys Ser Arg Glu Arg Arg Ala Pro Thr Pro Gin Arg Ala Gly Ser 
275 280 285 



-61- 



Pro Leu Pro Arg Thr Ser Arg Asp His His Arg Ser Pro Ser Pro Arg 
290 295 300 

Glu 
305 



<210> 139 
<211> 212 
<212> PRT 

<213> Haemophilus influenzae 
<40b> 139 

Met Lys Lys Thr Leu Leu Gly Ser Leu lie Leu Leu Ala Phe Ala Gly 
15 10 15 

Asn Val Gin- Ala Ala Ala Asn Ala Asp Thr Ser Gly Thr Val Thr Phe 
20 25 30 

Phe Gly Lys Val Val Glu Asn Thr Cys Gin Val Asn Gin Asp Ser Glu 
35 40 45 

Tyr Glu Cys Asn Leu Asn Asp Val Gly Lys Asn His Leu Ser Gin Gin 
50 55 60 

Gly Tyr Thr Ala Met Gin Thr Pro Phe Thr lie Thr Leu Glu Asn Cys 
65 70 75 80 

Asn Val Thr Thr Thr Asn Asn Lys Pro Lys Ala Thr Lys Val Gly Val 
85 90 95 

Tyr Phe Tyr Ser Trp Glu lie Ala Asp Lys Asp Asn Lys Tyr Thr Leu 
100 105 110 

Lys Asn lie Lys Glu Asn Thr Gly Thr Asn Asp. Ser Ala Asn Lys Val 
115 120 125 

Asn lie Gin Leu Leu Glu Asp Asn Gly Thr Ala Glu He Lys Val Val 
130 135 140 

Gly Lys Thr Thr Thr Asp Phe Thr Ser Glu Asn His Asn Gly Ala Gly 
145 150 155 160 

Ala Asp Pro Val Ala Thr Asn Lys His He Ser Ser Leu Thr Pro Leu 
165 170 175 

Asn Asn Gin Asn Ser He Asn Leu His Tyr He Ala Gin Tyr Tyr Ala 
180 185 190 

Thr Gly Val Ala Glu Ala Gly Lys Val Pro Ser Ser Val Asn Ser Gin 
195 200 205 

He Ala Tyr Glu 
210 



<210> 140 
<211> 139 
<212> PRT 

<213> Pseudomonas stutzeri 



<400> 140 

Met Lys Ala Gin Met Gin Lys Gly Phe Thr Leu He Glu Leu Met He 



-62- 



15 10 15 

Val Val Ala He He Gly He Leu Ala Ala He Ala Leu Pro Ala Tyr 
20 25 30 

Gin Asp Tyr Thr Val Arg Ser Asn Ala Ala Ala Ala Leu Ala Glu He 
35 40 45 

Thr Pro Gly Lys He Gly Phe Glu Gin Ala He Asn Glu Gly Lys Thr 
50 55 60 

Pro Ser Leu Thr Ser Thr Asp Glu Gly Tyr He Gly He Thr Asp Ser 
65 70 75 80 

Thr Ser Tyr Cys Asp Val Asp Leu Asp Thr Ala Ala Asp Gly His He 
85 90 95 

Glu Cys Thr Ala Lys Gly Gly Asn Ala Gly Lys Phe Asp Gly Lys Thr 
100 105 110 

He Thr Leu Asn Arg Thr Ala Asp Gly Glu Trp Ser Cys Ala Ser Thr 
115 120 125 

Leu Asp Ala Lys Tyr Lys Pro Gly Lys Cys Ser 
130 135 



<210> 141 
<211> 59 
<212> PRT 

<213> Caulobacter crescentus 
<400> 141 

Met Thr Lys Phe Val Thr Arg Phe Leu Lys Asp Glu Ser Gly Ala Thr 
15 10 15 

Ala He Glu Tyr Gly Leu He Val Ala Leu He Ala Val Val He Val 
20 25 30 

Thr Ala Val Thr Thr Leu Gly Thr Asn Leu Arg Thr Ala Phe Thr Lys 
35 40 45 

Ala Gly Ala Ala Val Ser Thr Ala Ala Gly Thr 
50 55 



<210> 142 
<211> 173 
<212> PRT 

<213> Escherichia coli 
<400> 142 

Met Ala Val Val Ser Phe Gly Val Asn Ala Ala Pro Thr He Pro Gin 
15 10 15 

Gly Gin Gly Lys Val Thr Phe Asn Gly Thr Val Val Asp Ala Pro Cys 
20 25 30 

Ser He Ser Gin Lys Ser Ala Asp Gin Ser He Asp Phe Gly Gin Leu 
35 40 45 

Ser Lys Ser Phe Leu Glu Ala Gly Gly Val Ser Lys Pro Met Asp Leu 
50 55 60 



-63- 



Asp He Glu Leu Val Asn Cys Asp He Thr Ala Phe Lys Gly Gly Asn 
65 70 ' • 75 80 

Gly Ala Gin Lys Gly Thr Val Lys Leu Ala Phe Thr Gly Pro He Val 
85 90 95 

Asn Gly His Ser Asp Glu Leu Asp Thr Asn Gly Gly Thr Gly Thr Ala 
100 105 110 

He Val Val Gin Gly Ala Gly Lys Asn Val Val Phe Asp Gly Ser Glu 
115 120 125 

Gly Asp Ala Asn Thr Leu Lys Asp Gly Glu Asn Val Leu His Tyr Thr 
130 135 140 

Ala Val Val Lys Lys Ser Ser Ala Val Gly Ala Ala Val Thr Glu Gly 
145 150 155 160 

Ala Phe Ser Ala Val Ala Asn Phe Asn Leu Thr Tyr Gin 
165 170 



<210> 143 
<211> 173 
<212> PRT 

<213> Escherichia coli 



<400> 143 

Met Ala Val Val Ser Phe Gly Val Asn Ala 
1 5 10 



Ala Pro Thr He 



Pro Gin 
15 



Gly Gin Gly Lys Val Thr 
20 



Phe Asn Gly Thr Val Val Asp Ala Pro Cys 
25 30 



Ser He Ser Gin Lys Ser Ala Asp 
35 40 



Gin Ser He Asp Phe Gly Gin Leu 
45 



Ser Lys Ser Phe Leu Glu 
50 

Asp He Glu Leu Val Asn 
65 70 

Gly Ala Gin Lys Gly Thr 
85 

Asn Gly His Ser Asp Glu 
100 

He Val Val Gin Gly Ala 
115 

Gly Asp Ala Asn Thr Leu 
130 

Ala Val Val Lys Lys Ser 
145 150 



Ala Gly 
55 

Cys Asp 
Val Lys 
Leu Asp 



Gly Lys 
120 



Gly Val Ser Lys Pro Met Asp Leu 
60 



He Thr Ala Phe Lys Gly 
75 



Leu Ala 
90 

Thr Asn 
105 



Phe Thr Gly Pro 



Gly Asn 
80 

He Val 
95 



Gly Gly Thr Gly Thr Ala 
110 



Asn Val Val Phe Asp Gly Ser Glu 
125 



Lys Asp 
135 



Gly Glu 
Ser Ala Val Gly 



Asn Val Leu His Tyr Thr 

140 



Ala Phe Ser Ala Val Ala Asn Phe Asn Leu 
165 170 



Ala Ala Val Thr 
155 

Thr Tyr Gin 



Glu Gly 
160 



<210> 144 
<211> 172 



-64- 



<212> PRT 

<213> Escherichia coli 



<400> 144 

Met Ala Val Val Ser Phe Gly Val 
1 5 



Asn Ala Ala Pro 
10 



Thr Thr Pro Gin 
15 



Gly Gin Gly Arg Val Thr Phe Asn 
20 



Gly Thr Val Val 
25 



Asp Ala Pro Cys 
30 



Ser He Ser Gin Lys Ser Ala Asp 
35 40 



Gin Ser He Asp 



Phe Gly Gin Leu 
45 



Ser Lys Ser Phe Leu Ala Asn Asp 
50 55 



Gly Gin Ser Lys 
60 



Pro Met Asn Leu 



Asp lie Glu Leu Val Asn Cys Asp 
65 70 

Ala Lys Thr Gly Ser Val Lys Leu 
85 



He Thr Ala Phe 
75 

Ala Phe Thr Gly 
90 



Lys Asn Gly Asn 
80 

Pro Thr Val Ser 
95 



Gly His Pro Ser Glu Leu 
100 



Ala Thr Asn Gly Gly Pro 
105 



Gly Thr Ala He 

110 



Met He Gin Ala 'Ala Gly Lys Asn 
115 120 



Val Pro Phe Asp 



Gly Thr Glu Gly 
125 



Asp Pro Asn Leu Leu Lys Asp Gly 
130 135 



Asp Asn Val Leu 
140 



His Tyr Thr Thr 



Val Gly Lys Lys Ser Ser 
145 150 



Asp Gly Asn Ala Gin He 
155 



Thr Glu Gly Ala 
160 



Phe Ser Gly Val Ala Thr 
165 



Phe Asn Leu Ser Tyr Gin 
170 



<210> 145 
<211> 853 
<212> DNA 

<213> Escherichia coli 

<220> 

<221> CDS 

<222> (281) . . (829) 

<400> 145 

acgtttctgt ggctcgacgc atcttcctca ttcttctctc caaaaaccac ctcatgcaat 60 

ataaacatct ataaataaag ataacaaata gaatattaag ccaacaaata aactgaaaaa 120 

gtttgtccgc gatgctttac ctctatgagt caaaatggcc ccaatgtttc atcttttggg 180 

ggaaactgtg cagtgttggc agtcaaactc gttgacaaac aaagtgtaca gaacgactgc 240 

ccatgtcgat ttagaaatag ttttttgaaa ggaaagcagc atg aaa att aaa act 295 

Met Lys He Lys Thr 
1 5 

ctg gca ate gtt gtt ctg teg get ctg tec etc agt tct aeg aeg get 343 
Leu Ala He Val Val Leu Ser Ala Leu Ser Leu Ser Ser Thr Thr Ala 
10 15 20 



-65- 



ctg gcc get gcc acg acg gtt aat ggt ggg acc gtt cac ttt aaa ggg 391 

Leu Ala Ala Ala Thr Thr Val Asn Gly Gly Thr Val His Phe Lys Gly 
25 30 35 

gaa gtt gtt aac gcc get tgc gca gtt gat gca ggc tct gtt gat caa 4 39 

Glu Val Val Asn Ala Ala Cys Ala Val Asp Ala Gly Ser Val Asp Gin 
40 45 50 



acc gtt cag tta gga cag gtt cgt acc gca teg ctg gca cag gaa gga 487 
Thr Val Gin Leu Gly Gin Val Arg Thr Ala Ser Leu Ala Gin Glu Gly 
55 60 65 

gca ace agt tct get gtc ggt ttt aac att cag ctg aat gat tgc gat 535 
Ala Thr Ser Ser Ala Val Gly Phe Asn lie Gin Leu Asn Asp Cys Asp 
70 75 80 85 

acc aat gtt gca tct aaa gcc get gtt gcc ttt tta ggt acg geg att 583 
Thr Asn Val Ala Ser Lys Ala Ala Val Ala Phe Leu Gly Thr Ala lie 
90 95 100 

gat gcg ggt cat acc aac gtt ctg get ctg cag agt tea get geg ggt 631 
Asp Ala Gly His Thr Asn Val Leu Ala Leu Gin Ser Ser Ala Ala Gly 
105 110 115 

age gca aca aac gtt ggt gtg cag ate ctg gac aga acg ggt get gcg 67 9 
Ser Ala Thr Asn Val Gly Val Gin lie Leu Asp Arg Thr Gly Ala Ala 
120 125 130 

ctg acg ctg gat ggt gcg aca ttt agt tea gaa aca ace ctg aat aac 727 
Leu Thr Leu Asp Gly Ala Thr Phe Ser Ser Glu Thr Thr Leu Asn Asn 
135 140 ■ 145 

gga ace aat acc att ecg tte cag gcg cgt tat ttt gca ace ggg gee 775 
Gly Thr Asn Thr He Pro Phe Gin Ala Arg Tyr Phe Ala Thr Gly Ala 
150 155 160 165 

gca- ace ecg ggt get get aat gcg gat gcg acc ttc aag gtt cag tat 823 
Ala Thr Pro Gly Ala Ala Asn Ala Asp Ala Thr Phe Lys Val Gin Tyr 
170 175 180 

caa taa cetaectagg ttcagggacg ttea 853 
Gin 



<210> 146 
<211> 182 
<212> PRT 

<213> Escherichia coli 



<400> 146 



Met 


Lys 


He 


Lys 


Thr 


Leu 


Ala 


He 


Val 


Val 


Leu 


Ser 


Ala 


Leu 


Ser 


Leu 


1 




5 










10 










15 




Ser 


Ser 


Thr 


Thr 


Ala 


Leu 


Ala 


Ala 


Ala 


Thr 


Thr 


Val 


Asn 


Gly 


Gly 


Thr 








20 










25 










30 






Val 


His 


Phe 


Lys 


Gly 


Glu 


Val 


Val 


Asn 


Ala 


Ala 


Cys 


Ala 


Val 


Asp 


Ala 






35 










40 










45 








Gly 


Ser 


Val 


Asp 


Gin 


Thr 


Val 


Gin 


Leu 


Gly 


Gin 


Val 


Arg 


Thr 


Ala 


Ser 




50 










55 










60 










Leu 


Ala 


Gin 


Glu 


Gly 


Ala 


Thr 


Ser 


Ser 


Ala 


Val 


Gly 


Phe 


Asn 


He 


Gin 


65 








70 










75 










80 


Leu 


Asn 


Asp 


Cys 


Asp 


Thr 


Asn 


Val 


Ala 


Ser 


Lys 


Ala 


Ala 


Val 


Ala 


Phe 










85 










90 










95 




Leu 


Gly Thr 


Ala 


He 


Asp 


Ala 


Gly 


-His 


Thr 


Asn 


Val 


Leu 


Ala 


Leu 


Gin 



-66- 



100 

Ser Ser Ala Ala Gly 
115 

Arg Thr Gly Ala Ala 
130 

Thr Thr Leu Asn Asn 
145 

Phe Ala Thr Gly Ala 
165 

Phe Lys Val Gin Tyr 
180 



105 

Ser Ala Thr Asn Val Gly 
120 

Leu Thr Leu Asp Gly Ala 
135 

Gly Thr Asn Thr lie Pro 
150 155 
Ala Thr Pro Gly Ala Ala 
170 

Gin 



110 

Val Gin lie Leu Asp 
125 

Thr Phe Ser Ser Glu 
140 

Phe Gin Ala Arg Tyr 
160 

Asn Ala Asp Ala Thr 
175 



<210> 147 
<211> 11 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> FLAG peptide 
<400> 147 

Cys Gly Gly Asp Tyr Lys Asp Asp Asp Asp Lys 
1 5 10 



<210> 148 
<211> 31 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> primer 
<400> 148 

ccggaattca tggacattga cccttataaa g 31 



<210> 149 
<211> 37 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> primer 
<400> 149 

gtgcagtatg gtgaggtgag gaatgctcag gagactc 37 



<210> 150 
<211> 37 
<212> DNA 
<213> Artificial 

<220> 

<223> primer 



Sequence 



<400> 150 

gsgtctcctg agcattcctc acctcaccat actgcac 



37 



-67- 



<210> 151 
<211> 33 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> primer 
<400> 151 

cttccaaaag tgagggaaga aatgtgaaac cac 33 



<210> 152 
<211> 47 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> primer 
<400> 152 

cgcgtcccaa gcttctaaac aacagtagtc tccggaagcg ttgatag 47 



<210> 153 
<211> 33 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> primer 
<400> 153 

gtggtttcac atttcttccc tcacttttgg aag 33 



<210> 154 
<211> 281 
<212> PRT 

<213> Saccharomyces cerevisiae 
<400> 154 

Met Ser Glu Tyr Gin Pro Ser Leu Phe Ala Leu Asn Pro Met Gly Phe 
15 10 15 

Ser Pro Leu Asp Gly Ser Lys Ser Thr Asn Glu Asn Val Ser Ala Ser 
20 25 30 

Thr Ser Thr Ala Lys Pro Met Val Gly Gin Leu lie Phe Asp Lys Phe 
35 40 45 

lie Lys Thr Glu Glu Asp Pro lie lie Lys Gin Asp Thr Pro Ser Asn 
50 55 60 

Leu Asp Phe Asp Phe Ala Leu Pro Gin Thr Ala Thr Ala Pro Asp Ala 
65 70 75 80 

Lys Thr Val Leu Pro lie Pro Glu Leu Asp Asp Ala Val Val Glu Ser 
85 90 95 

Phe Phe Ser Ser Ser Thr Asp Ser Thr Pro Met Phe Glu Tyr Glu Asn 
100 105 110 



-68- 



Leu Glu Asp Asn Ser Lys Glu Trp 
115 120 

Pro Val Thr Thr Asp Asp Val Ser 
130 135 

Thr Glu Glu Val Ser Leu Val Pro 
145 150 

Ser Phe Leu Pro Thr Pro Val Leu 
165 



Thr Ser Leu Phe Asp Asn Asp lie 
125 

Leu Ala Asp Lys Ala lie Glu Ser 
140 

Ser Asn Leu Glu Val Ser Thr Thr 
155 160 

Glu Asp Ala Lys Leu Thr Gin Thr 
170 175 



Arg Lys Val Lys Lys Pro Asn Ser Val Val Lys Lys Ser His His Val 
180 185 190 

Gly Lys Asp Asp Glu Ser Arg Leu Asp His Leu Gly Val Val Ala Tyr 
195 200 205 

Asn Arg Lys Gin Arg Ser He Pro Leu Ser Pro He Val Pro Glu Ser 
210 215 220 

Ser Asp Pro Ala Ala Leu Lys Arg Ala Arg Asn Thr Glu Ala Ala Arg 
225 230 235 240 

Arg Ser Arg Ala Arg Lys Leu Gin Arg Met Lys Gin Leu Glu Asp Lys 
245 250 255 

Val Glu Glu Leu Leu Ser Lys Asn Tyr His Leu Glu Asn Glu Val Ala 
260 265 270 

Arg Leu Lys Lys Leu Val Gly Glu Arg 
275 280 

<210> 155 
<211> 181 
<212> PRT 

<213> Escherichia coli 
<400> 155 

Met Lys He Lys Thr Leu Ala He Val Val Leu Ser Ala Leu Ser Leu 
15 10 15 

Ser Ser Thr Ala Ala Leu Ala Ala Ala Thr Thr Val Asn Gly Gly Thr 
20 25 30 

Val His Phe Lys Gly Glu Val Val Asn Ala Ala Cys Ala Val Asp Ala 
35 40 45 

Gly Ser Val Asp Gin Thr Val Gin Leu Gly Gin Val Arg Thr Ala Ser 
50 55 60 

Leu Ala Gin Glu Gly Ala Thr Ser Ser Ala Val Gly Phe Asn He Gin 
65 70 75 80 

Leu Asn Asp Cys Asp Thr Asn Val Ala Ser Lys Ala Ala Val Ala Phe 
85 ' 90 95 

Leu Gly Thr Ala He Asp Ala Gly His Thr Asn Val Leu Ala Leu Gin 
100 105 110 

Ser Ser Ala Ala Gly Ser Ala Thr Asn Val Gly Val Gin He Leu Asp 
115 120 125 



-69- 



Arg Thr Gly Ala Ala Leu Thr Leu Asp Gly Ala Thr Phe Ser Ser Glu 
130 135 140 

Thr Thr Leu Asn Asn Gly Thr Asn Thr lie Pro Phe Gin Ala Arg Tyr 
145 150 155 160 

Phe Ala Gly Ala Ala Thr Pro Gly Ala Ala Asn Ala Asp Ala Thr Phe 
165 170 175 

Lys Val Gin Tyr Gin 
180 



<210> 156 
<211> 447 
<212> DNA 
<213> Hepatitis B 

<220> 

<221> CDS 

<222> (1) . . (447) 

<400> 156 

atg gac att gac cct tat aaa gaa ttt gga get act gtg gag tta etc 48 

Met Asp lie Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

teg ttt ttg cct tct gac ttc ttt cct tec gta cga gat ett eta gat 96 
Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

ace gee gca get etg tat egg gat gee tta gag tct cct gag eat tgt 14 4 
Thr Ala Ala Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

tea eet cac cat act gca etc agg caa gca att ctt tge tgg gga gac 192 
Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp 
50 55 60 

tta atg act eta get ace tgg gtg ggt act aat tta gaa gat eca gea 240 
Leu Met Thr Leu Ala Thr Trp Val Gly Thr Asn Leu Glu Asp Pro Ala 
65 70 75 80 

tct agg gac eta gta gte agt tat gtc aac act aat gtg ggc eta aag 288 
Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Val Gly Leu Lys 
85 90 95 

ttc aga caa tta ttg tgg ttt cac att tct tgt etc act ttt gga aga 336 
Phe Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

gaa acg gtt eta gag tat ttg gte tct ttt gga gtg tgg att egc act 384 
Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

cct cea gee tat aga eca eca aat gee cct ate eta tea acg ctt eeg 432 
Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

gag act act gtt gtt 447 

Glu Thr Thr Val Val 

145 



<210> 157 
<211> 149 
<212> PRT 
<213> Hepatitis B 

<400> 157 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ala Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Thr Asn Leu Glu Asp Pro Ala 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Val Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Thr Val Val 
145 



<210> 158 
<211> 152 
<212> PRT 
<213> Hepatitis B 

<400> 158 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20- 25 30 ' 

Thr Ala Ala Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp 
50 55 - 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Thr Asn Leu Glu Asp Gly Gly 
65 70 75 80 

Lys Gly Gly Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Val 
85 90 95 

Gly Leu Lys Phe Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr 



-71- 



100 105 110 

Phe Gly Arg Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp 
115 120 125 

lie Arg Thr Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser 
130 135 140 

Thr Leu Pro Glu Thr Thr Val Val 
145 150 



<210> 159 

<211> 56 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Oligonucleotide 

<400> 159 

tagatgatta cgccaagctt ataatagaaa tagttttttg aaaggaaagc agcatg 56 



<210> 160 

<211> 45 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Oligonucleotide 

<400> 160 

gtcaaaggcc ttgtcgacgt tattccatta cgcccgtcat tttgg 45 



<210> 161 

<211> 4623 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> pFIMAIC 



<400> 161 

agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt 60 

tcttagacgt caggtggcac ttttcgggga -aatgtgcgcg gaacccctat ttgtttattt 120 

ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 180 

taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 240 

tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 300 

gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 360 



-72- 





gttt t cgccc 


cgaagaacgt 


tttccaatga 


tgagcacttt 


taaagttctg 


420 


ct atatCTQCcr 


cggtattatc 


ccgtattgac 


gccgggcaag 


agcaactcgg 


tcgccgcata 


480 


PPl a 1" 1" Cl" P 


acraatcractt 


aat taacrtac 


tcaccagtca 


cagaaaagca 


tcttacggat 


540 


nncrii'nriCrin 


1" aacracraatt 


atgcagtgct 


gccataacca 


tgagtgataa 


cactgcggcc 


600 




t" na pa a p era t 


cggaggaccg 


aaggagctaa 


ccgctttttt 


gcacaacatg 


660 




taactcacct 


tgatcgttgg 


gaaccggagc 


tgaatgaagc 


cataccaaac 


720 


era raacr cert CT 

y d M ^ J J 


acaccacgat 


gcctgtagca 


atggcaacaa 


cgttgcgcaa 


actattaact 


780 


rrrfpnaap1"ap 


tl" a p 1" pt aac 


ttcccggcaa 


caattaatag 


actggatgga 


ggcggataaa 


840 




pa p"t" 1" p1" rrpn 


pl"pcropppt"t' 


ccggctggct 


ggtttat tgc 


tgat aaatct 


900 




ciyoy uyyy l-v^ 


"t" PCTprfrrl" a1" p 

v_v^y^yy odk.v.<> 


attcrpaacac 

Ci C C- ^ V> Ci ^ Ci \^ 


tcracroccaaa 


tggt aagccc 


960 


't"ppprri~ai~pn' 


1"arr1"1"a1~p1"a 


^ d y Ci v.^ ^ ^ ^ ^ 


agtcaggcaa 


ctatggatga 


acgaaataga 


1020 


^ctyd L^yo Ly 


aya. L-Ciyy uyv^ 


O L. v> Ci w Ci 


aagcat tggt 


aact gtcaga 


ccaagtt tac 


1080 


1"pai"a1"a"t"ap 


t'arra1"1"aa 


ttt aaaactt 

C* C> C* Ci C* Ci Ci V— ' c c 


catttttaat 


ttaaaaggat 


ctaggtgaag 


1140 




a-r-aa-f-pi-pn-h 


gaccaaaatc 


ccttaacgtg 


agttttcgtt 


ccactgagcg 


1200 


■f~p^rT;^pppprf 


"harraaaarrai" 
L-Ciyciciciciyci 


pa a a ncra 1" pt* 


t cttgagatc 


ctttttttct 


gcgcgtaatc 


1260 


■t'rrr'"l~rfr''t"i"rfr' 
Lyt- LyL- L L.yL- 


ClClCl^ClClCl.ClClCl 


appapprrpta 


ppaerperatoa 


tttgtttgcc 


ggatcaagag 


1320 


pi~-^pp/^;^pi"p 


1" 1* "t" "t" 1" ppcraa 


crat aactaac 

^ ^ ^ U Ci C-f C- 


ttcagcagag 


cgcagatacc 


aaatactgtc 


1380 


pttptanlrrt" 


a crppcrl" acrl' 1" 


aggccaccac 


t tcaagaact 


ctgtagcacc 


gcctacatac 


1440 


ptprrptplrrp 


taat pptcrtl" 


accagtggct 


gctgccagtg 


gcgataagtc 


gtgtcttacc 


1500 


nnnt t rrna p1" 

yyy ^yyu^i^i.^ 


paacraccrata 


gttaccggat 


aaggcgcagc 


ggtcgggctg 


aacacraaaot 


1560 


+"prri~rfp^^pap 

L.v,^y uy^ov^civ^ 


anpppacip1"1" 


crcracrccraaccr 

\^ ^ Ci ^ ^h-^ y Ci Ci Vh^ ^ 


acctacaccg 


aactgagata 


cctacagcgt 


1620 


rf a rrpt* a 1" fia rr 


aaaopQppap 

d d d ^ \^ ^ d W 


get tcccgaa 


gggagaaagg 


cggacaggta 


tccggtaagc 


1680 


yyociyyy L-^y 


yddv^dyydyd 


ncQCri pcracrcr 


gagcttccag 


ggggaaacgc 


ctggtatctt 


1740 


■t-p+-an"t'pp1~n 

LQ LCiy U^L-fLy 


'Loyyy u^^y 


rcricct pt era 


cttera acatc 

C*' ^ ^ CJ Ci C^ \M C- \^ 


aatttttata 


atgctcgt ca 


1800 




rrpp1"a1"firraa 

y^o i-d i,yydd 


a CL CL V> ^ ^ CL ^ w 


aacgcggcct 


ttttacggtt 


cctggccttt 


1860 


"hrrp'hrrrfpp't" 
i-y^_rL.yyv^^i> u 


"t""hrrp1'papa't" 

u uy^ L.\^d^d L. 


attctttcct 


gcgttatccc 


ct gat t ctgt 


ggataaccgt 


1920 


;^t'"h;^pprrpp't' 




tnatappof^t 

i^yd i^dv^v^yVj- ^ 


cgccgcagcc 


gaacgaccga 


gcgcagcgag 


1980 


tcagtgagcg 


aggaagcgga 


agagcgccca 


atacgcaaac 


cgcctctccc 


cgcgcgttgg 


2040 


ccgattcatt 


aatgcagctg 


gcacgacagg 


tttcccgact 


ggaaagcggg 


cagtgagcgc 


2100 


aacgcaatta 


atgtgagtta 


gctcactcat 


taggcacccc 


aggctttaca 


ctttatgctt 


2160 


ccggctcgta 


tgttgtgtgg 


aattgtgagc 


ggataacaat 


ttcacacagg 


aaacagctat 


2220 


gaccatgatt 


acgccaagct 


tataatagaa 


atagtttttt 


gaaaggaaag 


cagcatgaaa' 


2280 



-73- 



attaaaactc tggcaatcgt tgttctgtcg gctctgtccc tcagttctac agcggctctg 2340 

gccgctgcca cgacggttaa tggtgggacc gttcacttta aaggggaagt tgttaacgcc 24 00 

gcttgcgcag ttgatgcagg ctctgttgat caaaccgttc agttaggaca ggttcgtacc 24 60 

gcatcgctgg cacaggaagg agcaaccagt tctgctgtcg gttttaacat tcagctgaat 2520 

gattgcgata ccaatgttgc atctaaagcc gctgttgcct ttttaggtac ggcgattgat 2580 

gcgggtcata ccaacgttct ggctctgcag agttcagctg cgggtagcgc aacaaacgtt 2640 

ggtgtgcaga tcctggacag aacgggtgct gcgctgacgc tggatggtgc gacatttagt 2700 

tcagaaacaa ccctgaataa cggaaccaat accattccgt tccaggcgcg ttattttgca 2760 

accggggccg caaccccggg tgctgctaat gcggatgcga ccttcaaggt tcagtatcaa 2820 

taacctaccc aggttcaggg acgtcattac gggcagggat gcccaccctt gtgcgataaa 2880 

aataacgatg aaaaggaaga gattatttct attagcgtcg ttgctgccaa tgtttgctct 2940 

ggccggaaat aaatggaata ccacgttgcc cggcggaaat atgcaatttc agggcgtcat 3000 

tattgcggaa acttgccgga ttgaagccgg tgataaacaa atgacggtca atatggggca 3060 

aatcagcagt aaccg.gtttc atgcggttgg ggaagatagc gcaccggtgc cttttgttat 3120 

tcatttacgg gaatgtagca cggtggtgag tgaacgtgta ggtgtggcgt ttcacggtgt 3180 

cgcggatggt aaaaatccgg atgtgctttc cgtgggagag gggccaggga tagccaccaa 3240 

tattggcgta gcgttgtttg atgatgaagg aaacctcgta ccgattaatc gtcctccagc 3300 

aaactggaaa cggctttatt caggctctac ttcgctacat ttcatcgcca aatatcgtgc 3360 

taccgggcgt cgggttactg gcggcatcgc caatgcccag gcctggttct ctttaaccta 3420 

tcagtaattg ttcagcagat aatgtgataa caggaacagg acagtgagta ataaaaacgt 3480 

caatgtaagg aaatcgcagg aaataacatt ctgcttgctg gcaggtatcc tgatgttcat 3540 

ggcaatgatg gttgccggac gcgctgaagc gggagtggcc ttaggtgcga ctcgcgtaat 3600 

ttatccggca gggcaaaaac aagagcaact tgccgtgaca aataatgatg aaaatagtac 3660 

ctatttaatt caatcatggg tggaaaatgc cgatggtgta aaggatggtc gttttatcgt 3720 

gacgcctcct ctgtttgcga tgaagggaaa aaaagagaat accttacgta ttcttgatgc 3780 

aacaaataac caattgccac aggaccggga aagtttattc tggatgaacg ttaaagcgat 3840 

tccgtcaatg gataaatcaa aattgactga gaatacgcta cagctcgcaa ttatcagccg 3900 

cattaaactg tactatcgcc cggctaaatt agcgttgcca cccgatcagg ccgcagaaaa 3960 

attaagattt cgtcgtagcg cgaattctct gacgctgatt aacccgacac cctattacct 4020 

gacggtaaca gagttgaatg ccggaacccg ggttcttgaa aatgcattgg tgcctccaat 4080 

gggcgaaagc acggttaaat tgccttctga tgcaggaagc aatattactt accgaacaat 4140 

aaatgattat ggcgcactta cccccaaaat gacgggcgta atggaataac gtcgactcta 4200 



-74- 

gaggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg 4260 

gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 4320 

cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 4380 

gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 4440 

tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg 4500 

ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc" cggcatccgc ttacagacaa 4560 

gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 4 620 

gcg 4623 



162 
42 
DNA 

Artificial Sequence 
<220> 

<223> Oligonucleotide 
<400> 162 

aagatcttaa gctaagcttg aattctctga cgctgattaa cc 42 



<210> 163 

<211> 41 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Oligonucleotide 

<400> 163 

acgtaaagca tttctagacc gcggatagta atcgtgctat c 41 



<210> 164 

<211> 5681 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> pFIMD 



<210> 
<211> 
<212> 
<213> 



<400> 164 

tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag 60 

gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg 120 

cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga • 180 

caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 240 



-75- 



ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 300 

gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 360 

gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 420 

atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 480 

caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 540 

gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 600 

accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 660 

ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 720 

gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 780 

acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 840 

atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 900 

ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 960 

gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 1020 

gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 1080 

tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 1140 

taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 1200 

cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1260 

gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1320 

gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1380 

agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1440 

aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1500 

agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1560 

cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1620 

accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1680 

aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1740 

ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 1800 

cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 1860 

gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 1920 

tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 1980 

agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 2040 

aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 2100 

gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 2160 



-76- 



ccccaggctt 


tacactttat 


gcttccggct 


cgtatgttgt 


gtggaattgt 


gagcggataa 


2220 


caatttcaca 


caggaaacag 


ctatgaccat 


gattacgcca 


agcttgaatt 


ctctgacgct 


2280 


gattaacccg 


acaccctatt 


acctgacggt 


aacagagttg 


aatgccggaa 


cccgggttct 


2340 


tgaaaatgca 


ttggtgcctc 


caatqqqcqa 


aagcacggtt 


aaattgcctt 


ctgatgcagg 


2400 


aagcaatat t 


acttaccgaa 


caataaatga 


ttatggcgca 


cttaccccca 


aaatgacggg 


2460 


cgtaatggaa 


taacgcaggg 


ggaatttttc 


gcctgaataa 


aaagaattga 


ctqccqqqqt 


2520 


gattttaagc 


cggaggaata 


atgtcatatc 


tgaatttaag 


actttaccag 


cgaaacacac 


2580 


fieit act t aCei 

O. O L- \^ w v-J w Ci 


tat tcgtaag 


cat cgtttgg 


ctggtttttt 


tgtccgactc 


gttgtcgcct 


2640 


gtgcttttgc 


cgcacaggca 


cctttgtcat 


ctgccgacct 


ctattttaat 


ccgcgctttt 


2700 


taacQaa taa 

^ >^ ^ ^ >J V-* 


tccccaggct 


gtggccgatt 


tatcgcgttt 


tqaaaatqqq 


caagaattac 


2760 


cgccagggac 


gtatcgcgtc 


gatatctatt 


tgaataatgg 


ttatatggca 


acgcgtgatg 


2820 




tarrinrrrnar 


agtgaacaag 


ggat tgttcc 


ctgcctgaca 


cgcgcgcaac 


2880 


L- V-^ ^ Ci. M Ci 


ggggctgaat 


acggcttctg 


tcgccggtat 


gaatctgctg 


gcggatgatg 


2940 


pd" q1" crl" occ 


attaaccaca 


atggtccagg 


acgctactgc 


gcatctggat 


qttqqtcaqc 


3000 


aacaactaaa 

Ci ^ M ^ V-^ I— ^ t_A 


cctgacgatc 


cctcaggcat 


ttatgagtaa 


tcgcgcgcgt 


ggttatattc 


3060 


ct cctgagtt 


atgggatccc 


ggtattaatg 


ccggattgct 


caattataat 


ttcagcggaa 


3120 


atagtgtaca 


gaatcggatt 


gggggtaaca 


gccattatgc 


atatttaaac 


ctacagagtg 


3180 


ggttaaatat 


taatacataa 


cgtttacgcg 


acaataccac 


ctggagttat 


aacagtagcg 


3240 


fiCri ClPi t CPit C 

CJ. O C-4 v_i l_ ^ k_ ^ 


aggtagcaaa 


aataaatggc 


agcatatcaa 


tacctggctt 


gagcgagaca 


3300 


-t- a a tar rot t 


acattcccaa 


ctgacgctgg 


gtgatggtta 


tactcagggc 


gatattttcg 


3360 


t OCT t* a t*taa 


ctttcgcggc 


gcacaattgg 


cctcagatga 


caatatgtta 


cccgatagtc 


3420 


aaaaaacrat t 

t*. t* U ^ ^ ^ 


tgccccggtg 


atccacggta 


ttgctcgtgg 


tactgcacag 


gtcactatta 


3480 


aacaaaatgg 


gtatgacatt 


tataatagta 


cggtgccacc 


qqqqcctttt 


accatcaacg 


3540 


ata tctat gc 


cgcaggtaat 


agtggtgact 


tgcaggtaac 


gatcaaagag 


gctgacggca 


3600 


gcacgcagat 


tt t taccgt a 


ccctattcgt 


cagtcccgct 


tttgcaacgt 


gaagggcata 


3660 




rattaracrca 


ggagaat a cc 


gtagtggaaa 


tgcgcagcag 


gaaaaaaccc 


3720 


act t ttt cca 


gagtacatta 


ctccacggcc 


ttccggctgg 


ctggacaata 


tatggtggaa 


3780 


cgcaactggc 


ggatcgttat 


cgtgctttta 


atttcggtat 


cgggaaaaac 


atgggggcac 


3840 


tgggcgctct 


gtctgtggat 


atgacgcagg 


ctaattccac 


acttcccgat 


gacagtcagc 


3900 


atgacggaca 


atcggtgcgt 


tttctctata 


acaaatcgct 


caatgaatca 


ggcacgaata 


3960 


ttcagttagt 


gggttaccgt 


tattcgacca 


gcggatattt 


taatttcgct 


gatacaacat 


4020 


acagtcgaat 


gaatggctac 


aacattgaaa 


cacaggacgg 


agttattcag 


gttaagccga 


4080 



-77- 



CiCL L. L-V^Cl^^^Cl 




^^L-^y^L. uaud 


r5 a 3 copcra 

d \ji \^ y-A u 


era aai"1"3pa3 

^ C4. CL CL ^ ^ CL \^ CL CL 


p1"pappa1"'t'f^ 


4140 




^yyyL*yL<ci^d 


uL^ddL>dWL.y L. 


ciL.L.L.ycLy L.yy 


1"30PPr3l"P3a 

L.CLV^V^^CL L.^_rClCl 


dL'L udu ^yyy 


4200 




'I~rri"r'n;=i1"nprr 
L,y L,v_ryci L.yciy 


^ciciu. L-v^^ciyy 


p1"C7a3 1" i" 3 ^5 

CJ ^ C_-l Ci CI 


i" ;3 p 1" cr p cr 1" "t" p 


c\F^p{ npi 1" pa 

ycLCiycL L,CL i^v^cl 


4260 




L^dyo L.CI L.ciyv^ 


\j L.ydoyu.ciuci 


uv^y^-rV^ L.yy^ci 


ciCLCLCLyyci.v^yy 


ydLL>dyduy l. 


4320 




prri'r'^fl't'fl'l"'!" 




a pt* rrnpi" npn 

dVrf uyy^ uy^y 


t" 1* pt" rra Pr5 rft" 

\_ ^ycL^ciy ^ 


dddLWLL'Ciy L 


4380 




r'3rfi~rfr'p;^rrp 


l_ d CL u \^ C-i \J l_ 


papapnai*p"t* 


pa a pcroi" prrn 


a 1" rra ppaa P 

d u CL \— ' CL d O 


4 4 40 




a Ldoyy uci^y 


'\"\' net nnpirin 

I. uywi^yyctciy 


aP^^SPr^/^PPt" 


PriCrPl"3l"r5CrP 
CL ^ O w CL ^ CL u 


ntripaaappcr 

^ \^ d d d w 


4500 




yyydyy^.^yci. u 


yyddd L-ciy^-'y 


rrs ^^rft*apr3rfrf 

yciuy ^dv-rCiyy 


p1"aprfPPr^prf 


plrraa ■t"1"a t"P 

uydd I- i-d i-w 


4560 




n n (~* p i~ rr 
L-.yyodd LyL-t^ 


dd Ld UL>yy l l 


pp;^rfpp;^i"pirT 
dody^^d udy 


prr^i~rrP'l~;^'l~'l~ 
^^yci. uyci uo l. l. 


a a rr pa rrp"t" p"t* 

clcLyocly^L,^u<■l_ 


4 620 


dLLdL-yyciy l 


L*dyL.yy uyyy 


y Ld^-'Lyy^ 


d uy^L'dd Lyy 


prr"t"?i^^prrp"l"rr 
v^y L. CL ci ^ y w y 


yyyody^^y l. 


4 680 


Lda.a.CyaL.a(^ 


yytyyuycLL 


y L LdddyL-yu, 


i_ LyyL-yoddd 


dyd Ly^dddd 


(Thprraaaspp 
y Li^yddddoo 


4740 


a /"T ^ /-^ /"T /~T /~T "f" 

dydcyyyyy l 


yoy Ldoi— ydo 


uyyoy i_yy u l. 


;^"t"rrppn"t"np"t' 
dL.y^^v^y uyv^L. 


rrpp'l~'l~;^i~npp 


api~rfaa'l"ai~p 
CLv^uydd i^u i—v^ 


4800 


yyydddd Ldy 


dy Lyyoyt- Ly 


yd Ld^L^L-dd ud 


ppp'hrfO'p'hn'^ 
ooL-uyyouyd 


LddL-y LL-ydu 


■t""t"arrpi1~aaprr 
u i^dyci i_dd^y 


4860 


cyyLtycLdd 


t^y LLy ULUL-L- 


dLj LL^y Lyyyy 


oydLv— y uy^^y 


dyodydy l l l 


a a rrprrprfprr 
dddyL-yL-yoy 


4 920 


4- 4- r~r '2: '\~ Ih 1^ 

L uyy y a Uddd 


dctycLOaty 


doyoLydL-ot^ 


dU-dd Ldd Ldd 


rfpprTpi"rfpprf 
yL-wyt— Lyv^t^y 


L L uyyyyL.yd 


4 980 


UyyLyaCdLC 


dydydy Ldyw 


L-dydy Ldyi^y 


y^^duuy 1- Lyo 


yyd LddLyy l 


parTrTi"'hi~ppp 
v_<cj.yy \- i_ i-civ_-o 


5040 


"f" ^ 4~ 

ccdycyyddL 


gcc L L t.d,yuy 


yyddddy l 


dyy Lyddd Ly 


yyydyddydy 


ydddd LyL- LL- 


5100 


dCLyLyLCyC 


CadLLdLCdd 


Lyc-odi^L-dy 


dydy LL-dyL-d 


yL-dy L Ld L Ld 


dL-^^^^dy*^ Ld L 


5160 


odyo Lydd L.y 


Luy L Lddyyy 


yy^y i^yd uyd 


ycicici^ci.cici.o\^ 


ft tttatctt 


p't"cr't"crpnp1"1" 


5220 


LLL Ly LyyoL. 


yyoyy uyay \~ 


od^yih'L u Lyy 


\^ v.y<i^yyci. L.ci.y 


P3 prra t* t" r5 p1" 

CL CL L« Ik^ CL \^ ^ 


f5't"ppcrpoo1"p 


5280 


Ldydyyd ll-l. 


L-oyyy LdL-i^y 


dyLvLi^yddu i- 


pj^p'hrrrrpprr'l" 
od^LyyL>L>y l 


n nt 1 1 1 ^ n p\ r\ 
oy t_ I- L. l.ci^ci.cl 


p(Tt"prri~rr^ip't" 


5340 


yyyddddv^L^o 


LyyL«y l LdL-o 


v^ddo u udd Lv— 


npp"t"1"fTparrp 
yLrL-L L-yLrdy*^ 


A P ;=3 1" P P P P P t 


ttpcrccaapt 


5400 


ggcgtaatag 


cgaagaggcc 


cgcaccgatc 


gcccttccca 


acagttgcgc 


agcctgaatg 


5460 


gcgaatggcg 


cctgatgcgg 


tattttctcc 


ttacgcatct 


gtgcggtatt 


tcacaccgca 


5520 


tatggtgcac 


tctcagtaca 


atctgctctg 


atgccgcata 


gttaagccag 


ccccgacacc 


5580 


cgccaacacc 


cgctgacgcg 


ccctgacggg 


cttgtctgct 


cccggcatcc 


gcttacagac 


5640 


aagctgtgac 


cgtctccggg 


agctgcatgt 


gtcagaggtt 


t 




5681 



<210> 165 

<211> 40 

<212> DNA 

<213> .Artificial Sequence 



<220> 

<223> Oligonucleotide 



-78- 



<400> 165 

aattacgtga gcaagcttat gagaaacaaa cctttttatc 40 



<210> 166 

<211> 41 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Oligonucleotide 

<400> 166 

gactaaggcc tttctagatt attgataaac aaaagtcacg c 41 



<210> 167 

<211> 4637 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> pFIMFGH 



<400> 167 
aaagggcctc 


gtgatacgcc 


tatttttata 


ggttaatgtc 


atgataataa 


tggtttctta 


60 


gacgtcaggt 


ggcacttttc 


ggggaaatgt 


gcgcggaacc 


cctatttgtt 


tatttttcta 


120 


aatacattca 


aatatgtatc 


cgctcatgag 


acaataaccc 


tgataaatgc 


ttcaataata 


180 


ttgaaaaagg 


aagagtatga 


gtattcaaca 


tttccgtgtc 


gcccttattc 


ccttttttgc 


240 


ggcattttgc 


cttcctgttt 


ttgctcaccc 


agaaacgctg 


gtgaaagtaa 


aagatgctga 


300 


agatcagttg 


ggtgcacgag 


tgggttacat 


cgaactggat 


ctcaacagcg 


gtaagatcct 


360 


tgagagtttt 


cgccccgaag 


aacgttttcc 


aatgatgagc 


acttttaaag 


ttctgctatg 


420 


tggcgcggta 


ttatcccgta 


ttgacgccgg 


gcaagagcaa 


ctcggtcgcc 


gcatacacta 


480 


ttctcagaat 


gacttggttg 


agtactcacc 


agtcacagaa 


aagcatctta 


cggatggcat 


540 


gacagtaaga 


gaattatgca 


gtgctgccat 


aaccatgagt 


gataacactg 


cggccaactt 


600 


acttctgaca 


acgatcggag 


gaccgaagga 


gctaaccgct 


tttttgcaca 


acatggggga 


660 


tcatgtaact 


cgccttgatc 


gttgggaacc 


ggagctgaat 


gaagccatac 


caaacgacga 


720 


gcgtgacacc 


acgatgcctg 


tagcaatggc 


aacaacgttg 


cgcaaactat 


taactggcga 


780 


actacttact 


ctagcttccc 


ggcaacaatt 


aatagactgg 


atggaggcgg 


ataaagttgc 


840 


aggaccactt 


ctgcgctcgg 


cccttccggc 


tggctggttt 


attgctgata 


aatctggagc 


900 


cggtgagcgt 


gggtctcgcg 


gtatcattgc 


agcactgggg 


ccagatggta 


agccctcccg 


960 


tatcgtagtt 


atctacacga 


cggggagtca 


ggcaactatg 


gatgaacgaa 


atagacagat 


1020 



-79- 

cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 1080 

tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 1140 

ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 1200 

ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 1260 

cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 1320 

aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 1380 

agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 1440 

tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 1500 

ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 1560 

cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 1620 

atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 1680 

ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag . 1740 

tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 1800 

gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 1860 

gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 1920 

cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 1980 

gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 2040 

tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 2100 

aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 2160 

tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca 2220 

tgattacgcc aagcttatga gaaacaaacc tttttatctt ctgtgcgctt ttttgtggct 2280 

ggcggtgagt cacgctttgg ctgcggatag cacgattact atccgcggct atgtcaggga 2340 

taacggctgt agtgtggccg ctgaatcaac caattttact gttgatctga tggaaaacgc 2400 

ggcgaagcaa tttaacaaca ttggcgcgac gactcctgtt gttccatttc gtattttgct 2460 

gtcaccctgt ggtaatgccg tttctgccgt aaaggttggg tttactggcg ttgcagatag 2520 

ccacaatgcc aacctgcttg cacttgaaaa tacggtgtca gcggcttcgg gactgggaat 2580 

acagcttctg aatgagcagc aaaatcaaat accccttaat gctccatcgt ccgcgctttc 2640 

gtggacgacc ctgacgccgg gtaaaccaaa tacgctgaat ttttacgccc ggctaatggc 2700 

gacacaggtg cctgtcactg cggggcatat caatgccacg gctaccttca ctcttgaata 2760 

tcagtaactg gagatgctca tgaaatggtg caaacgtggg tatgtattgg cggcaatatt 2820 

ggcgctcgca agtgcgacga tacaggcagc cgatgtcacc atcacggtga acggtaaggt 2880 

cgtcgccaaa ccgtgtacgg tttccaccac caatgccacg gttgatctcg gcgatcttta 2940 



-80- 



ft rtt traat 


^--L. v,d i-^*-y 


v_»v««yyyyv^yy 


atcggcctgg 


catgatgt tg 


cgcttgagtt 


3000 


CIR ct RRt t at 

<j ci ^ c-i ci ^ v_j 


rracrtoaoaa 

^^^^^ ^ 


cgt cgagggt 


cactgccagc 


ttcagcgggg 


cagccgacag 


3060 


tacccf9^3tat 


tataaaaacc 


aggggaccgC 


gcaaaacatc 


cagttagagc 


tacaggatga 


3120 


racrtaacaac 

W ^ ^ ^ M w u u 


acattaaata 


ctggcgcaac 


caaaacagtt 


caggtggatg 


a t tcct caca 


3180 


ai"panpnpap 


i"1"rprcr1"i"ar' 

^ y u> 1^ d 


agg^cagagc 


attgacagta 


aat ggcggag 


ccactcaggg 


3240 




rrparr1"rra1"1"a 


^ ^ \^ \^ \^ 


tacctacagc 


tgaacccgaa 


gaga t gat tg 


3300 


"t'aa't'rraaapn 
ucici uyciciciv^y 


an1"1"a1"1"arr 


ctatttactcr 

^ y ^ ^ ^ y \^ y 


tactactaat 


gggctggt eg 


gtaaatgcct 


3360 


yy m.ct l LU-y*^ 


i^uy Lddddo^ 


y^(-»dd uyy ud 


ppnpi'a'hppp 

o o y X— ci ^ V — 


'hat' ^ nncncif' 

i^dv_ L,yyv^yy i— 


nncRncnccFi 

y y o d y o y o \^ d 


3420 


;^fn1-i-1-;Rl-n1- 
duyi-LLciLyL. 


dduoo u Ly^-^y 


v^vj.\-.y L^v^y i_yci. 


ai~rfi~rrrTnTrpa 

ci L-.y L-yyyyv_yCi 


aaappi"Qcr1~p 

d d d \^ w L. y y 


otcroatptt'l" 

y L« y y d v> l. o v_ 


3480 


u>yct^y^^cictci u 




a a pcra t" 1" a 1" c 


V> \J U <-4. O U ^ 


tacaaactat 


gt cacactgc 


3540 


dciL^yciyyoL^ 


yy^u udL-yy^^ 


rfrTprrt'n1~'t"a"h 

yy^^-y y ^ l^cii- 


rtaatttttc 


QgggQCCgta 


aaatatagtg 


3600 


yt^ciy L-cxy^L-ci 


■|"pr';^"t"t""t"pr'i~ 


apparrarrccr 

o. \^ d ^-Hf ^— ' a. ^ 


aaacgccgcg 


cgttgtttat 


aattcgagaa 


3660 




y uyyooyy uy 


y^^yk^L. uL-dUL. 


uydVi^y\«'0 t-y i- 


rra rrpan1"crpcr 

ydyv^dy *^y^^y 


y y ^y y y y '-y y 


3720 


oyd L Ldddy^ 


yy LOd L ud 


a"t"'l~rrr'pn"l~rrp 

C3> L.y^^y L.yv.f 


ttattttaca 

L^i^uL^e ^wy ^ y 


acagaccaac 


aactataaca 


3780 


yuydLydLLL 


oi^dy L i_ L.y uy 


uyydd L.d L. L. u 


^pnppaai'aa 

Q ^ y ^ Q. a L, Ci ci 


't"nai"rfl"rrrri~n 
Lya y ^yy ^y 


rftTTPPt'aptcr 


3840 


yoyyoLyoyd 


uy L.L. i_^-«i-y^-'i- 


rTf'hrfi^'hrri'pa 

^y L.yciL.y l.v^ci 


pp rfi" 1" a p"t" p1" ■ 


appcraaptac 


cctQattcaa 


3900 


LyL'Odd L LL*^^ 


u^uudOL-yuL 


i~p'M*rrt"rrr'rra 
^y oyv^yo. 


aaarrppaaaa 

ci ci ci y w w cj. a a 


ppI" cracTQl" r3 1" 

\w >J y y ^ 


tacctctccg 


3960 


yodL^ddi^u-y^ 


dyd uy^-'yyy^-' 


ddi_.ui-^yd u i_ i_ 


I— Q <— • vj a. a u a 


V-* <^ vJ 1— V-^ ^ \_ 1^ 


^ d l_ d 


4020 


dyyyi^y Loyy 


v>y L-oociy c ^y 


apnpfipaapcr 

civ — yVw^yv^ci-Ci y 


cr1"apcrattat 


t ccaacaaat 


aacacggtat 


4080 


oy L Ldyydy^ 


dy i_dyyyd\— u 


v-^yy^k^yy eye* 


cri" p1" nnCei 1 1 

y e y y y d ii^ 


aacggcaaat 


tatgcacgta 


4140 


t^i_yyayyyt^,d 


yy Lydt—Ly^—d 


yyydd L.y L.yL* 


aa+*pna1"i~a't" 

ClCie^yClL. L.CLL. 


1"rrf7pni*na pt" 

L.yy^y *_ydo i- 


tttatttatc 


4200 


dd Ldd Li— Ldy 


dyyd L.ov^v-'V-'y 


rrrfhapprrarrp 

yy L.ci^^yciyv^ 


1" paa a 1" 1" pa p 


t cjncc.cit.cat. 


tttacaacot 

^ ^ ^ V--' t#i ^ 


4260 


uy Lydu i-yyy 


ddddOLfO i-yy 


oy L L-d^k^odd 


p't"t'aa1"pnpp 


W M CA u ^ ^ta^ 


1" PPPPPt ftp 


4320 


accaactaac 

^ V-" ^PP^ tpA ^ V-i' ^ M M ^ 


gtaa tagcga 


agaggcccgc 


accgatcgcc 


cttcccaaca 


attqcqcaac 


4380 


ctgaatggcg 


aatggcgcct 


gatgcggtat 


tttctcctta 


cgcatctgtg 


cggtatttca 


4440 


caccgcatat 


ggtgcactct 


cagtacaatc 


tgctctgatg 


ccgcatagtt 


aagccagccc 


4500 


cgacacccgc 


caacacccgc 


tgacgcgccc 


tgacgggctt 


gtctgctccc 


ggcatccgct 


4560 


tacagacaag 


ctgtgaccgt 


ctccgggagc 


tgcatgtgtc 


agaggttttc 


accgtcatca 


4620 


ccgaaacgcg 


cgagacg 










4637 



<210> 
<211> 



168 
9299 



-81- 



<212> DNA 

<213> Artificial Sequence 
<220> 

<223> pFIMAICDFGH 



cgagacgaaa 


gggcctcgtg 


atacgcctat 


ttttataggt 


taatgtcatg 


ataataatgg 


60 


tttcttagac 


gtcaggtggc 


acttttcggg 


gaaatgtgcg 


cggaacccct 


atttgtttat 


120 


ttttctaaat 


acattcaaat 


atgtatccgc 


tcatgagaca 


ataaccctga 


taaatgcttc 


180 


aataatattg 


aaaaaggaag 


agtatgagta 


ttcaacattt 


ccgtgtcgcc 


cttattccct 


240 


tttttgcggc 


attttgcctt 


cctgtttttg 


ctcacccaga 


aacgctggtg 


aaagtaaaag 


300 


atgctgaaga 


tcagttgggt 


gcacgagtgg 


gttacatcga 


actggatctc 


aacagcggta 


360 


agatccttga 


gagttttcgc 


cccgaagaac 


gttttccaat 


gatgagcact 


tttaaagttc 


420 


tgctatgtgg 


cgcggtatta 


tcccgtattg 


acgccgggca 


agagcaactc 


ggtcgccgca 


480 


tacactattc 


tcagaatgac 


ttggttgagt 


actcaccagt 


cacagaaaag 


catcttacgg 


540 


atggcatgac 


agtaagagaa 


ttatgcagtg 


ctgccataac 


catgagtgat 


aacactgcgg 


600 


ccaacttact 


tctgacaacg 


atcggaggac 


cgaaggagct 


aaccgctttt 


ttgcacaaca 


660 


tgggggatca 


tgtaactcgc 


.cttgatcgtt 


gggaaccgga 


gctgaatgaa 


gccataccaa 


720 


acgacgagcg 


tgacaccacg 


atgcctgtag 


caatggcaac 


aacgttgcgc 


aaactattaa 


780 


ctggcgaact 


acttactcta 


gcttcccggc 


aacaattaat 


agactggatg 


gaggcggata 


840 


aagttgcagg 


accacttctg 


cgctcggccc 


ttccggctgg 


ctggtttatt 


gctgataaat 


900 


ctggagccgg 


tgagcgtggg 


tctcgcggta 


tcattgcagc 


actggggcca 


gatggtaagc 


960 


cctcccgtat 


cgtagttatc 


tacacgacgg 


ggagtcaggc 


aactatggat 


gaacgaaata 


1020 


gacagatcgc 


tgagataggt 


gcctcactga 


ttaagcattg 


gtaactgtca 


gaccaagttt 


1080 


actcatatat 


actttagatt 


gatttaaaac 


ttcattttta 


atttaaaagg 


atctaggtga 


1140 


agatcctttt 


tgataatctc 


atgaccaaaa 


tcccttaacg 


tgagttttcg 


ttccactgag 


1200 


cgtcagaccc 


cgtagaaaag 


atcaaaggat 


cttcttgaga 


tccttttttt 


ctgcgcgtaa 


1260 


tctgctgctt 


gcaaacaaaa 


aaaccaccgc 


taccagcggt 


ggtttgtttg 


ccggatcaag 


1320 


agctaccaac 


tctttttccg 


aaggtaactg 


gcttcagcag 


agcgcagata 


ccaaatactg 


1380 


tccttctagt 


gtagccgtag 


ttaggccacc 


acttcaagaa 


ctctgtagca 


ccgcctacat 


1440 


acctcgctct 


gctaatcctg 


ttaccagtgg 


ctgctgccag 


tggcgataag 


tcgtgtctta 


1500 


ccgggttgga 


ctcaagacga 


tagttaccgg 


ataaggcgca 


gcggtcgggc 


tgaacggggg 


1560 


gttcgtgcac 


acagcccagc 


ttggagcgaa 


cgacctacac 


cgaactgaga 


tacctacagc 


1620 



-82- 



gtgagctatg 


agaaagcgcc 


acgcttcccg 


aaqqqaqaaa 


ggcqgacagg 

3 3 3 3 3 3 


tatccggtaa 


1680 


gcggcagggt 


cggaacagga 


gagcgcacga 


gggagcttcc 


aqqqqqaaac 

^33333^^^^ 


gcctggtatc 


1740 


tttatagtcc 


tgtcgggt tt 


cgccacctct 


gacttgagcg 


tcgatttttg 


tgatgctcgt 


1800 


CdiQQQQQQCQ 


aaacctataq 


aaaaacgcca 


gcaacgcggc 


ctttttacgg 


ttcctggcct 


1860 


tttgctggcc 


ttttgctcac 


atgttctttc 


ctgcgttatc 


ccctgattct 


gtggataacc 


1920 


gtattaccgc 


ctttgagtga 


gctgataccg 


ctcgccgcag 


ccgaacgacc 


qaqcqcaqcq 


1980 


agt cagtgag 


cgaggaagcg 


gaagagcgcc 


caatacgcaa 


accgcctctc 


cccgcgcgtt 


2040 


ggccgattca 


ttaatgcagc 


tggcacgaca 


ggtttcccga 


ctqgaaaqcq 


qqcaqtqaqc 


2100 


gcaacgcaat 


taatgtgagt 


tagctcactc 


attaggcacc 


ccaggcttta 


cactttatgc 


2160 


t tcccractca 


tatattatat 


ggaattgtga 


gcggataaca 


atttcacaca 


ggaaacagct 


2220 


at gaccatga 


ttacgccaag 


cttataatag 


aaatagtttt 


ttgaaaggaa 


agcagcatga 


2280 


aaattaaaac 


tctggcaa t c 


gttgttctgt 


cggctctgtc 


cctcagttct 


acagcggctc 


2340 


t nnccoct.oci 

^ ^ U W \^ \^ W I— ^ V--" 


cacQaccrat t 

w c-i y v-^ y ^— 


aatggtggga 


ccgttcactt 


taaaggggaa 


gttgttaacg 


2400 


rrcrrt*1"crccfr 


aattaat aca 


ggctctgttg 


atcaaaccgt 


tcagttagga 


caggttcgta 


2460 




vJ d M d CJ. 


ggagcaacca 


gt tctgctgt 


cggttttaac 


attcagctga 


2520 


;=)'l~rf;^'l~'t~rTpn^ 

a. L-^^ci I- L.^^v^>^cl. 


Ci w ^ d ^ ^ 


gcatctaaag 


ccgctgt tgc 


ctttttaggt 


acggcgattg 


2580 




taccaacott 

U V— " v." d. CL ^ L- 


ctggctctgc 


agagttcagc 


tgcgggtagc 


gcaacaaacg 


2640 


ttggtgt gca 


gatcct ggac 


agaacgggtg 


ctgcgctgac 


actaaatqqt 


gcgacattta 


2700 


gttcagaaac 


aaccct gaat 


aacggaacca 


ataccattcc 


qttccaqqcq 


cgttattttg 


2760 


caaccggggc 


cgcaaccccg 


ggtgctgcta 


atgcggatgc 


gaccttcaag 


gttcagtatc 


2820 


aataacctac 


ccaggttcag 


ggacgtcatt 


acqqqcaqqq 


atgcccaccc 


ttgtgcgata 


2880 


aaaataacga 


tgaaaaggaa 


gagattattt 


ctattagcgt 


cgttgctgcc 


aatgtttgct 


2940 


^ L# ^ ^ \^ w M ^ ^ ^ 


ataaat ggaa 


taccacgttg 


cccggcggaa 


atatgcaatt 


tcaqqqcqtc 

^^"^33^^3 


3000 


1" 1" 1" 1" npciQ 


aaacttgccg 


gat tgaagcc 


ggtgataaac 


aaatgacggt 


caatatgggg 


3060 




ataaccaott 


tratacaatt 


ggggaagata 


gcgcaccggt 


gccttttgtt 


3120 


att catt tac 


gggaatgtag 


cacggtggtg 


agtgaacgtg 


taqqtatqqc 


gtttcacggt 


3180 


a t" c o c cr cr a t a 


gtaaaaatcc 


ggatgtgctt 


tccgtgggag 


aqqqqccaqq 


gatagccacc 


3240 


aatattggcg 


tagcgttgtt 


tgatgatgaa 


ggaaacctcg 


taccgattaa 


tcgtcctcca 


3300 


gcaaactgga 


aacggcttta 


ttcaggctct 


acttcgctac 


atttcatcgc 


caaatatcgt 


3360 


gctaccgggc 


gtcgggttac 


tggcggcatc 


gccaatgccc 


aggcctggtt 


ctctttaacc 


3420 


tatcagtaat 


tgttcagcag 


ataatgtgat 


aacaggaaca 


ggacagtgag 


taataaaaac 


3480 


gtcaatgtaa 


ggaaatcgca 


ggaaataaca 


ttctgcttgc 


tggcaggtat 


cctgatgttc 


3540 



-83- 



atggcaatga 


tagttqccgq 


acgcgctgaa 


gcgggagtgg 


ccttaggtgc 


gactcgcgta 


3600 


atttatccgg 


cagggcaaaa 


acaagagcaa 


cttgccgtga 


caaataatga 


tgaaaatagt 


3660 


acctatttaa 


ttcaatcatg 


ggtggaaaat 


gccgatggtg 


taaaggatgg 


tcgttttatc 


3720 


gtgacgcctc 


ctctgtttgc 


gatgaaggga 


aaaaaagaga 


ataccttacg 


tattcttgat 


3780 


gcaacaaata 


accaattgcc 


acaggaccgg 


gaaagtttat 


tctggatgaa 


cgttaaagcg 


3840 


attccgtcaa 


tggataaatc 


aaaattgact 


gagaatacgc 


tacagctcgc 


aattatcagc 


3900 


cgcattaaac 


tgtactatcg 


cccggctaaa 


ttagcgttgc 


cacccgatca 


ggccgcagaa 


3960 


aaattaagat 


ttcgtcgtag 


cgcgaattct 


ctgacgctga 


ttaacccgac 


accctattac 


4020 


ctgacggtaa 


cagagttgaa 


tgccggaacc 


cgggttcttg 


aaaatgcatt 


ggtgcctcca 


4080 


atgggcgaaa 


gcacggttaa 


attgccttct 


gatgcaggaa 


gcaatattac 


ttaccgaaca 


4140 


ataaatgatt 


atggcgcact 


tacccccaaa 


atqacqqqcq 


taatggaata 


acgcaggggg 


4200 


aatttttcgc 


ctgaataaaa 


agaattgact 


qccqqqqtqa 


ttttaagccg- 


gaggaataat 


4260 


gtcatatctg 


aatt taagac 


tttaccagcg 


aaacacacaa 


tgcttgcata 


ttcgtaagca 


4320 


t cgt ttggct 


ggtttttttg 


tccgactcgt 


tgtcgcctgt 


gcttttgccg 


cacaggcacc 


4380 


tttgtcatct 


gccgacctct 


attttaat'cc 


gcgcttttta 


gcggatgatc 


cccaggctgt 


4440 


ggccgattta 


tcgcgttttg 


aaaatgggca 


agaattaccg 


ccagggacgt 


atcgcgtcga 


4500 


tatctatttg 


aataatggtt 


atatggcaac 


qcqtqatqtc 


acatttaata 


cgggcgacag 


4560 


t gaacaaggg 


attgttccct 


gcctgacacg 


cgcgcaactc 


gccagtatgg 


ggctgaatac 


4620 


ggcttctgtc 


acccrqtatqa 


atctgctggc 


qqatqatgcc 


tgtgtgccat 


taaccacaat 


4680 


ggtccaggac 


gctactgcgc 


atctggatgt 


tqqtcaqcaq 


cgactgaacc 


tgacgatccc 


4740 


t caggca t tt 


atgagtaatc 




ttatattcct 


cctgagttat 


qggatcccgg 


4800 


t att aatgcc 


ggattgctca 


attataattt 


cagcggaaat 


agtgtacaga 


atcggattgg 


4860 


gggtaacagc 


cattatgcat 


atttaaacct 


acagagtggg 


ttaaatattg 


gtgcgtggcg 


4920 


tttacgcgac 


aataccacct 


ggagttataa 


cagtagcgac 


agatcatcag 


gtagcaaaaa 


4980 


taaatggcag 


catatcaata 


cctggcttga 


gcgagacata 


ataccgttac 


gttcccggct 


5040 


gacgctgggt 


gatggttata 


ctcagggcga 


tattttcgat 


ggtattaact 


ttcgcggcgc 


5100 


acaattggcc 


tcagatgaca 


atatgttacc 


cgatagtcaa 


aqaqqatttq 


ccccggtgat 


5160 


ccacggtatt 


gctcgtggta 


ctgcacaggt 


cactattaaa 


caaaatgggt 


atgacattta 


R o 0 n 


taatagtacg 


gtgccaccgg 


ggccttttac 


catcaacgat 


atctatgccg 


caggtaatag 


5280 


tggtgacttg 


caggtaacga 


tcaaagaggc 


tgacggcagc 


acgcagattt 


ttaccgtacc 


5340 


ctattcgtca 


gtcccgcttt 


tgcaacgtga 


agggcatact 


cgttattcca 


ttacggcagg 


5400 


agaataccgt 


agtggaaatg 


cgcagcagga 


aaaaacccgc 


tttttccaga 


gtacattact 


5460 



-84- 



y. 



ccacggcctt 


ccggctggct 


ggacaatata 


tqqtqqaacq 


caactggcgg 


atcgttatcg 


5520 


tgcttttaat 


ttcggtatcg 


ggaaaaacat 


qqqqqcactq 


qqcqctctqt 


ctgtggatat 


5580 


gacgcaggct 


aattccacac 


ttcccgatga 


cagtcagcat 


gacggacaat 


cggtgcgttt 


5640 


tctctat aac 


aaatcgctca 


atgaatcagg 


cacgaatatt 


caqttaqtqq 


gttaccgtta 


5700 


ttcgaccagc 


ggatatttta 


atttcgctga 


tacaacatac 


agtcgaatga 


atggctacaa 


5760 


cattgaaaca 


caacracacraa 


ttattcaggt 


taagccgaaa 


ttcaccgact 


attacaacct 


5820 


cgcttataac 


aaacgcggga 


aattacaact 


caccgttact 


cagcaactcg 


ggcgcacatc 


5880 


aacactgtat 


ttgagtggta 


gccatcaaac 


ttattgggga 


acgagtaatg 


tcgatgagca 


5940 


attccaggct 


ggattaaata 


ctgcgttcga 


agatatcaac 


tggacgctca 


gctatagcct 


6000 


gacgaaaaac 


gcctggcaaa 


aaggacggga 


tcagatgtta 


gcgcttaacg 


tcaatattcc 


6060 


ttt raarcac 

^ U ^ M w w <A w 


taactacatt 


ctgacagtaa 


atctcagtgg 


cgacatgcca 


gtgccagcta 


6120 


cagcatgtca 


cacgatct ca 


acggtcggat 


gaccaatctg 


qctqgtqtat 


acggtacgtt 


6180 


net GGRBOfiC 


aacaacct ca 


gctatagcgt 


gcaaaccggc 


tatgccgggg 


qaqqcqatqq 


6240 


aaa1" acrccraa 


agtacaggct 


acgccacgct 


gaattatcgc 


qqtqqttacQ 


gcaatgccaa 


6300 


1" ri t cnnt t 


agccatagcg 


atgatattaa 


gcagctctat 


tacggagtca 


qcqqtqqqqt 


6360 


p "t" CT cr r t" p i" 


gccaatggcg 


taacgctggg 


gcagccgtta 


aacgatacgg 


tqqtqcttqt 


6420 






al'crraaaaat 

C* ^ ^ (-A tA C-A \-A ^ 


cgaaaaccag 


acgggggtgc 


gtaccgactg 


6480 


gcgtggttat 


gccgtgctgc 


cttatgccac 


tgaatatcgg 


gaaaatagag 


tqqcqctqqa 


6540 


taccaatacc 


ctggctgata 


acgtcgattt 


agataacgcg 


gttgctaacg 


ttgttcccac 


6600 


t cataaaaco 


atcgtgcgag 


cagagtttaa 


agcgcgcgtt 


gggataaaac 


tgctcatgac 


6660 


gctgacccac 


aataataagc 


cgctgccgtt 


taaaacQatq 


gtgacatcag 


agagtagcca 


6720 


gagtagcggc 


attgttgcgg 


ataatggtca 


ggtttacctc 


agcggaatgc 


ctttagcggg 


6780 


aaaaattcacf 


crtaaaataaa 


gagaagagga 


aaatgctcac 


tgtgtcgcca 


attatcaact 


6840 


M O d. V.H' KA V-J Ci w 


aatcaacaac 

Q ^ tJi y ^ y V-* 


agt tattaac 


ccagctatca 


gctgaatgtc 


qttaaqqqqq 

3*-^'^'^33333 


6900 


CQtaat aacra 


aacaaacctt 


tttatcttct 


gtgcgctttt 


ttqtqqctqq 


cggtgagtca 


6960 


PCfr'M"t"CfcrPl" 


crrcraatacfca 

\^ u U C4 \^ d 


cgattactat 


ccgcggctat 


gtcagggata 


acggctgtag 


7020 


"t"n+"rrrTPprrp"h 




attttactat 


taatctaa ta 


gaaaacgcgg 


cgaagcaatt 


7080 


taacaacatt 


ggcgcgacga 


ctcctgttgt 


tccatttcgt 


attttgctgt 


caccctgt'gg 


7140 


taatgccgtt 


tctgccgtaa 


aggttgggtt 


tactggcgtt 


gcagatagcc 


acaatgccaa 


7200 


cctgcttgca 


cttgaaaata 


cggtgtcagc 


ggcttcggga 


ctgggaatac 


agcttctgaa 


7260 


tgagcagcaa 


aatcaaatac 


cccttaatgc 


tccatcgtcc 


gcgctttcgt 


ggacgaccct 


7320 


gacgccgggt 


aaaccaaata 


cgctgaattt 


ttacgcccgg 


ctaatggcga 


cacaggtgcc 


7380 



-85- 





tgtcactcfcg 


gggcatatca 


atgccacggc 


taccttcact 


cttgaatatc 


agtaactgga 


7440 




gatgctcatg 


aaatggtgca 


aacgtgggta 


tgtattggcg 


gcaatattgg 


cgctcgcaag 


7500 




tgcgacgata 


caacrcaacccr 


atgtcaccat 


cacggtgaac 


ggtaaggtcg 


tcgccaaacc 


7560 




. gtgtacggtt 


tccaccacca 


atgccacggt 


tgatctcggc 


gatctttatt 


ctttcagtct 


7620 




tatgtctgcc 


aaaacaacat 


cggcctggca 


tgatgttgcg 


cttgagttga 


ctaattgtcc 


7680 




ggtgggaacg 


tcgagggtca 


ctgccagctt 


caQcqgqqca 


gccgacagta 


ccggatatta 


7740 




faaaaaccacr 

U CI <-i. U ^ w <— i ^ 


^ y ^ ^ ^ 


aaaacatcca 


gttagagcta 


caggatgaca 


gtggcaacac 


7800 




a 1 1" aaat art 


ggcgcaacca 


aaacagt tea 


ggtggatgat 


tcctcacaat 


cagcgcactt 


7860 




cccgttacag 


gtcagagcat 


tgacagtaaa 


taacaaaacc 


actcagggaa 


ccattcaggc 


7920 




acft'crat' t aQC 


at cacctata 


cctacagctg 


. aacccgaaga 


gatgattgta 


atgaaacgag 


7980 




ttattaccct 


gtttgctgta 


ctgctgatgg 


actaatcqqt 


aaatgcctgg 


tcattcgcct 


8040 






raatcrat acp 

\^ d a. L. \-i 1^ \j w 


gctatcccta 


ttaqcqqtqq 


cagcgccaat 


gtttatgtaa 


8100 




accttgcgcc 


cgtcgtgaat 


gtggggcaaa 


acctggtcgt 


ggatctttcg 


acgcaaatct 


8160 


f: 




ma 1 1 a t rT'Q 


gaaaccatta 


cagactatgt 


cacactgcaa 


cqaaactcqq 


8220 






prTtrTttr^tr"t 


aatttttrra 


ggaccgtaaa 


atatagt ggc 


agtagctatc 


8280 




catttcctac 


caccagcgaa 


acgccgcgcg 


ttgtttataa 


ttcgagaacg 


gataagccgt 


8340 




nnccGClt CSdC 


gctttatttg 


acgcctgtga 


qcaqtqcqqq 




attaaagctg 


8400 




optcattaat 


tgccgtgct t 


attttgcgac 


agaccaacaa 


ctataacagc 


gatgatttcc 


8460 




acft'tt'crt'Cjta 


aaa tatttac 


gccaataatg 


atqtaqtqqt 


gcctactggc 


ggctgcgatg 


8520 




t't'tctartca 


tgatgtcacc 


gttactctgc 


cggactaccc 


tqqttcaqtq 


ccaattcctc 


8580 






ttntrrrnaaa 


agccaaaacc 


tggggtatta 


cctct ccggc 


acaaccgcag 


8640 




flt ncaciciCeiri 

d ~d M ^ ^ ^ ^ 


ctcgattt tc 


accaataccg 


cgtcgttttc 


acctgcacag 


qqcqtcaqcq 


8700 




1" a racrt t crac 


gcgcaacggt 


acgattatt c 


cagcgaataa 


cacggtatcg 


ttaggagcag 


8760 




taocraacttc 


aacaatcraot 


ctgggat taa 


cggcaaatta 


tgcacgtacc 


qqaqqqcaqq 


8820 




tgactgcagg 


gaat gtgcaa 


tcgattattg 


gcgtgacttt 


tgtttatcaa 


taatctagaa 


8880 






gtaccgagct 


cgaatt cact 


ggccgtcgtt 


ttacaacgtc 


qtqactqqqa 


8940 




aaaccctQac 

<-A U (-4 V** ^ ^ *^ V-*" 


gttacccaac 


ttaatcgcct 


tgcagcacat 


ccccctttcg 


ccagctggcg 


9000 




taatagcgaa 


gaggcccgca 


ccgatcgccc 


ttcccaacag 


ttgcgcagcc 


tgaatggcga 


9060 




atggcgcctg 


atgcggtatt 


ttctccttac 


gcatctgtgc 


ggtatttcac 


accgcatatg 


9120 




gtgcactctc 


agtacaatct 


gctctgatgc 


cgcatagtta 


agccagcccc 


gacacccgcc 


9180 




aacacccgct 


gacgcgccct 


gacgggcttg 


tctgctcccg 


'gcatccgctt 


acagacaagc 


9240 




tgtgaccgtc 


tccgggagct 


gcatgtgtca- 


gaggttttca 


ccgtcatcac 


cgaaacgcg 


9299 



<210> 169 

<211> 8464 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> pFIMAICDFG 





cgagacgaaa 


gggcctcgtg 


atacgcctat 




tttcttagac 


gtcaggtggc 


acttttcggg 




ttttctaaat 


acattcaaat 


atgtatccgc 




aataatattg 


aaaaaggaag 


agtatgagta 




tttttgcggc 


attttgcctt 


cctgtttttg 




atgctgaaga 


tcagttgggt 


gcacgagtgg 


2 


agatccttga 


gagttttcgc 


cccgaagaac 




tgctatgtgg 


cgcggtatta 


tcccgtattg 




tacactattc 


tcagaatgac 


ttggttgagt 


~, 


atggcatgac 


agtaagagaa 


ttatgcagtg 




ccaacttact 


tctgacaacg 


atcggaggac 




tgggggatca 


tgtaactcgc 


cttgatcgtt 




acgacgagcg 


tgacaccacg 


atgcctgtag 




ctggcgaact 


acttactcta 


gcttcccggc 




aagttgcagg 


accacttctg 


cgctcggccc 




ctggagccgg 


tgagcgtggg 


tctcgcggta 




cctcccgtat 


cgtagttatc 


tacacgacgg 




gacagatcgc 


tgagataggt 


gcctcactga 




actcatatat 


actttagatt 


gatttaaaac 




agatcctttt 


tgataatctc 


atgaccaaaa 




cgtcagaccc 


cgtagaaaag 


atcaaaggat 




tctgctgctt 


gcaaacaaaa 


aaaccaccgc 




agctaccaac 


tctttttccg 


aaggtaactg 




tccttctagt 


gtagccgtag 


ttaggccacc 



-86- 





Ldd i_y UL^d uy 


dUdd udd i_yy 


60 




oyyddv^v^v^o L, 


duv_i,y Li^udi^ 


120 




d L. d d V— ' v_» uyd 


l_ddd. L.y\^ U K^K^ 


180 


i- -h r p ^ r 3 -t- i- -f- 


L.y u^yo^ 


\^ \^ l_d L. l-^0<wL. 


24 0 




dduyi— (-yy Ly 


dddy L-ddddy 


300 


y u Ud^^d uoyd 


(jL.\^ uyydLt^u^-. 


ci ci wQ y i,^ y y l.ci 


360 


yLLLLUUddL 


yd Lydyi^dOL 


LLLdddy L LO 


4 ^ U 


doy^t-yyyL^d 


dydyk^ddk^ l. 


yy L-oyv^^^y^d 


480 


dOLv-dL-Ody L 


L-dt— dyddddy 


od L-doyy 


540 


LyuL-d Lddo 


^dL.ydy uyd l. 


ddod^uy^yy 


600 


Uyddyydyu L 


ddOL^yoLLL L 


u LyL'dL-dd^d 


660 


gggaaccgga 


gctgaatgaa 


gccataccaa 


720 


caatggcaac 


aacgttgcgc 


aaactattaa 


780 


aacaattaat 


agactggatg 


gaggcggata 


840 


ttccggctgg 


ctggtttatt 


gctgataaat 


900 


tcattgcagc 


actggggcca 


gatggtaagc 


960 


ggagtcaggc 


aactatggat 


gaacgaaata 


1020 


ttaagcattg 


gtaactgtca 


gaccaagttt 


1080 


ttcattttta 


atttaaaagg 


atctaggtga 


1140 


tcccttaacg 


tgagttttcg 


ttccactgag 


1200 


cttcttgaga 


tccttttttt 


ctgcgcgtaa 


1260 


taccagcggt 


ggtttgtttg 


ccggatcaag 


1320 


gcttcagcag 


agcgcagata 


ccaaatactg 


1380 


acttcaagaa 


ctctgtagca 


ccgcctacat 


1440 



-87- 



acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 1500 

ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 1560 

gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 1620 

gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 1680 

gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 1740 

tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 1800 

caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 1860 

tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 1920 

gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 1980 

agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 2040 

ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 2100 

^ .gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 2160 

S ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 2220 

m atgaccatga ttacgccaag cttataatag aaatagtttt ttgaaaggaa agcagcatga 2280 

rV aaattaaaac tctggcaatc gttgttctgt cggctctgtc cctcagttct acagcggctc 2340 

tggccgctgc cacgacggtt aatggtggga ccgttcactt taaaggggaa gttgttaacg 2400 

Q ccgcttgcgc agttgatgca ggctctgttg atcaaaccgt tcagttagga caggttcgta 24 60 

Zi ccgcatcgct ggcacaggaa ggagcaacca gttctgctgt cggttttaac attcagctga 2520 

^ atgattgcga taccaatgtt gcatctaaag ccgctgttgc ctttttaggt acggcgattg 2580 

i^. atgcgggtca taccaacgtt ctggctctgc agagttcagc tgcgggtagc gcaacaaacg 2640 

ttggtgtgca gatcctggac agaacgggtg ctgcgctgac gctggatggt gcgacattta 2700 

gttcagaaac aaccctgaat aacggaacca ataccattcc gttccaggcg cgttattttg 2760 

caaccggggc cgcaaccccg ggtgctgcta atgcggatgc gaccttcaag gttcagtatc 2820 

aataacctac ccaggttcag ggacgtcatt acgggcaggg atgcccaccc ttgtgcgata 2880 

aaaataacga tgaaaaggaa gagattattt ctattagcgt cgttgctgcc aatgtttgct 2940 

ctggccggaa ataaatggaa taccacgttg cccggcggaa atatgcaatt tcagggcgtc 3000 

attattgcgg aaacttgccg gattgaagcc ggtgataaac aaatgacggt caatatgggg 3060 

caaatcagca gtaaccggtt tcatgcggtt ggggaagata gcgcaccggt gccttttgtt 3120 

attcatttac gggaatgtag cacggtggtg agtgaacgtg taggtgtggc gtttcacggt 3180 

gtcgcggatg gtaaaaatcc ggatgtgctt tccgtgggag aggggccagg gatagccacc 3240 

aatattggcg tagcgttgtt tgatgatgaa ggaaacctcg taccgattaa tcgtcctcca 3300 

gcaaactgga aacggcttta ttcaggctct acttcgctac atttcatcgc caaatatcgt 3360 



-88- 



get accgggc 


gt cgggttac 


tggcggcatc 


gccaatgccc 


aqqcctqqtt 


ctctttaacc 


3420 


tatcagtaat 


tgttcagcag 


ataatgtgat 


aacaggaaca 


ggacagtgag 


taataaaaac 


3480 


gtcaatgtaa 


ggaaatcgca 


ggaaataaca 


ttctgcttgc 


tggcaggtat 


cctgatgttc 


3540 


atggcaatga 


toattacccra 


acgcgctgaa 


crcqqqaqtqq 


ccttaggtgc 


gactcgcgta 


3600 


att tatccgg 


cagggcaaaa 


acaagagcaa 


cttgccgtga 


c^aataatga 


tgaaaatagt 


3660 


acctat t taa 


ttcaatcatg 


ggtggaaaat 


qccqatqqtq 


taaaggatgg 


tcgttttatc 


3720 


gtgacgcctc 


ctctgtttgc 


gatgaaggga 


aaaaaagaga 


ataccttacg 


tattcttgat 


3780 


gcaacaaata 


accaattgcc 


acaggaccgg 


gaaagtttat 


tctggatgaa 


cgttaaagcg 


3840 


attccgtcaa 


tggataaatc 


aaaattgact 


gagaatacgc 


tacagctcgc 


aattatcagc 


3900 


cgcattaaac 


tgtactatcg 


cccggctaaa 


ttagcgttgc 


cacccgatca 


ggccgcagaa 


3960 


aaat taagat 


ttcgtcgtag 


cgcgaattct 


ctgacgctga 


ttaacccgac 


accctattac 


4020 


ctgacggtaa 


cagagttgaa 


tgccggaacc 


cgggttcttg 


aaaatgcatt 


ggtgcctcca 


4080 




npaprrcrt't'aa 

V ■ d ^ y L- d d 


attgcct tct 


gatgcaggaa 


gcaatattac 


ttaccgaaca 


4140 


□.1— C1CXC11.UCIL.L. 


aiTrrfprrpapl" 

d L. y y w y d w ^ 


tacccccaaa 


atgacgggcg 


taatggaata 


acgcaggggg 


4200 




ptcraataaaa 


agaat tgact 


gccggggtga 


ttttaagccg 


gaggaataat 


4260 




aa 1" 1" 1" aaaac 


t ttaccaaca 

^ ^ ^ w (-i \A w \A 


aaacacacaa 


tgcttgcata 


ttcgtaagca 


4320 


tcgtttggct 


ggtttttttg 


tccgactcgt 


tgtcgcctgt 


gcttttgccg 


cacaggcacc 


4380 


tttgtcatct 


gccgacct ct 


attttaatcc 


gcgcttttta 


gcggatgatc 


cccaggctgt 


4440 


crcfcccrattta 

^ ^ ^ ^ ^ t-J. ^— 


t cgcgttt tg 


aaaatgggca 


agaattaccg 


ccagggacgt 


atcgcgtcga 


4500 


tatctattta 


aataataatt 


atatggcaac 


gcgtgatgtc 


acatttaata 




4560 




attattpcct 

d ^ U ^ L-i 


gcctgacacg 


cgcgcaact c 


gccagtatgg 


ggctgaatac 


4620 


ggcttctgtc 


gccggtatga 


atctgctggc 


ggatgatgcc 


tgtgtgccat 


taaccacaat 


4680 


yy ^.o^ciyya^ 


octactcrcac 


atctcrcra t ot 


tggtcagcag 


cgactgaacc 


tgacgatccc 


4740 


t caaacatt t 

w ^ M ^ ^ ^ ^ ^ 


atgagtaatc 


gcgcgcgtgg 


ttatattcct 


cctgagttat 


qqqatcccqq 


4800 


1"a1"taatncr 


ggattgct ca 


attataattt 


cagcggaaat 


agtgtacaga 


atcggattgg 


4860 


nnrfhaaparrp 

LiddV^dy^ 


pa t* 1" a t*crp at 


att taaacct 


acagagtggg 


ttaaatattg 


qtacqtqqcq 


4920 


■hi-i-aprrpnap 

I- L. L-d^^^^d^ 


dd l^dVrfWdV^V^ V— 


rrnaattataa 


cagtagcgac 


agatcatcag 


gtagcaaaaa 


4980 


taaatggcag 


catatcaata 


cctggcttga 


gcgagacata 


ataccgttac 


gttcccggct 


5040 


gacgctgggt 


gatggttata 


ctcagggcga 


tattttcgat 


ggtattaact 


ttcgcggcgc 


5100 


acaattggcc 


tcagatgaca 


atatgttacc 


cgatagtcaa 


agaggatttg 


ccccggtgat 


5160 


ccacggtatt 


gctcgtggta 


ctgcacaggt 


cactattaaa 


caaaatgggt 


atgacattta 


5220 


taatagtacg 


gtgccaccgg 


ggccttttac 


catcaacgat 


atctatgccg 


caggtaatag 


5280 



-89- 



tggtgacttg 


caggtaacga 


tcaaagaggc 


tgacggcagc 


acgcagattt 


ttaccgtacc 


5340 


ctat t cgtca 


gt cccgcttt 


tgcaacgtga 


agggcatact 


cgttattcca 


ttacggcagg 


5400 




agtggaaatg 


cgcagcagga 


aaaaacccgc 


tttttccaga 


gtacattact 


5460 


ccacggcctt 


ccggctggct 


ggacaatata 


taataqaacq 


caactggcgg 


atcgttatcg 


5520 


tgcttttaat 


ttcggtatcg 


ggaaaaacat 


gggggcactg 


ggcgctctgt 


ctgtggatat 


5580 


gacgcaggct 


aatt ccacac 


ttcccgatga 


cagtcagcat 


gacggacaat 


cggtgcgttt 


5640 


tctctataac 


aaatcgctca 


atgaatcagg 


cacgaatatt 


cagttagtgg 


gttaccgtta 


5700 


t tcgaccagc 


ggatatttta 


atttcgctga 


tacaacatac 


agtcgaatga 


atggctacaa 


5760 


cattgaaaca 


caggacggag 


ttattcaggt 


taagccgaaa 


ttcaccgact 


attacaacct 


5820 


cgcttataac 


aaacgcggga 


aattacaact 


caccgttact 


cagcaactcg 


ggcgcacat c 


5880 






\^ \^ ^ d. G w a a a. w 


t tattgggga 


acgagtaatg 


tcga tgagca 


5940 


CIV. L.^V.^Cl.^uV^ L. 


aa^5l" t"aaata 


ctacattcaa 


agatatcaac 


tggacgctca 


gctatagcct 


6000 


\J Ci \^ \J d CL Q CI CI ^ 


V4 ^ ^ y y wC4 Gi 


aaggacggga 


t cagatgtta 


gcgcttaacg 


tcaatattcc 


6060 


I"'t'1"r'f^cir'r'r3r' 

Iw. l_ V_> w 


t" aapt'Opat t" 

^ ^ ^ 


ctgacagtaa 


atct cagtgg 


cgacatgcca 


gtgccagct a 


6120 




cacgatctca 


acggtcggat 


gaccaat ctg 


gctggtgtat 


acggtacgt t 


6180 




Cl. CL W d CI ^ v> ^ CL 


Qp 1" a 1" a a p cr t 


gcaaaccggc 


tatgccgggg 


gaggcgatgg 


6240 


CLdCl Ld^^^^O 


3 rrt" a p;^ rrnp 1" 


Cl \— J V— ^ V— ' Ci W C- 


craat tat cere 


ggtggttacg 


gcaatgccaa 


6300 


i";^'l"r'rTrf1~'l~;^r' 


;^rrpp3l"r3npcr 

d v_j \^ \^ d d Vh'' M 


atgatattaa 


gcagctctat 


tacggagtca 




6360 






1" a a pqpI" QCrcr 

^ d d y V-* ^ ^ M M 


gcagccgt ta 


aacgatacgg 


t ggtgcttgt 


6420 


taaacTcarct 

^ d u M ^ M ^ '-^ ^ 


^ ^ Vta^ y ^ t-l d IpA ^ 


atgcaaaagt 


cgaaaaccag 


acgggggtgc 


gtaccgactg 


6480 




appa1~actac 


ct t atgccac 


t gaatatcgg 


gaaaatagag 


tggcgctgga 


6540 


m. ^ (ji LA ^ (J. \^ v-i* 


ctggctgata 


acgtcgattt 


agataacgcg 


gttgctaacg 


ttgttcccac 


6600 


1" cotcrcracf cci 

L^V./^ ^^^^^ ^ ^ 


atcatacaaa 


cagagt t taa 


agcgcgcgtt 


gggataaaac 


tgctcatgac 


6660 


L.<^ClWOv^Cl\^ 


CI Cl wL d d \j \^ 


cgctgccgtt 


tggggcgatg 


gtgacat cag 


agagtagcca 


6720 


cracft"30PQQP 


at" tcrttacacr 


ataatggt ca 


ggtt tacct c 


agcggaatgc 


ctttagcggg 


6780 


d Q CI CI u w U' ^ CI u 


n1" craaa taaa 

u u CL ^ ^ M y y 


gagaagagga 


aaatgctcac 


tgtgtcgcca 


attatcaact 


6840 


npp^ipp^^n^n 


CL M G W ^ Vwir ^#4 ^ Xri^ 


aottattaap 


ccagctatca 


gctgaatgtc 


gttaaggggg 


6900 


cgtgatgaga 


aacaaacctt 


tttatcttct 


gtgcgctttt 


ttgtggctgg 


cggtgagtca 


6960 


cgctttggct 


gcggatagca 


cgattactat 


ccgcggctat 


gtcagggata 


acggctgtag 


7020 


tgtggccgct 


gaatcaacca 


attttactgt 


tgatctgatg 


gaaaacgcgg 


cgaagcaatt 


7080 


taacaacatt 


ggcgcgacga 


ctcctgttgt 


tccatttcgt 


attttgctgt 


caccctgtgg 


7140 


taatgccgtt 


tctgccgtaa 


aggttgggtt 


tactggcgtt 


gcagatagcc 


acaatgccaa 


7200 



-90- 





cctgcttgca 


ct tgaaaa ta 


cggtgtcagc 


ggcttcggga 


ctgggaatac 


agcttctgaa 


7260 




tgagcagcaa 


aatcaaatac 


cccttaatgc 


tccatcgtcc 


gcgctttcgt 


ggacgaccct 


7320 




Oei coccooot 

\^ a w y w ^ L- 


aaaccaaata 


cgctgaat t t 


ttacgcccgg 


ctaatggcga 


cacaggtgcc 


7380 




tgtcactgcg 


gggcatatca 


atgccacggc 


taccttcact 


cttgaatatc 


agtaactgga 


7440 




gatgctcatg 


aaa tggtgca 


aacgt gggta 


tgtattggcg 


gcaatattgg 


cgctcgcaag 


7500 






\^ ci y y ^ y 


atgtcaccat 


cacggtgaac 


ggtaaggt eg 


tcgccaaacc 


7560 






tccac caeca 


atgccacggt 


tgatct cggc 


gatctttatt 


ctt tcagtct 


7620 




i'^'tn'tr'i'nnr' 


nnnncnncpif' 

yyyyv^yyv-fU. \- 


COnCCt OOC ri 

\^ y y \^ \^ ^ y y ^ ^ 


tgatgttgcg 


cttgagtt ga 


ctaattgtcc 


7680 




yy L.yyycici^y 


i" errs cincjf' cfi 


^i-y^v^fO.y**-'*-*— 


r* a cr c a crcrcr ca 


accaacaata 

^ ^ y ci. ci y CCl 


cc crcratatta 

^ y y CL C C*. C C CL 


7740 






yyyov^v^y^yv— ' 


RRRriCFit CCci 
a a <ji ci w a \^ v^ci 


gttagagcta 


caggatgaca 


gtggcaacac 


7800 




a u uyQci uaoi- 


yyoy^ciciv.j'^ci. 


PiririCfiCTtt Cri 

CL Q CL \^ CL y L« \^ U 


cra1"aaa1"cra1" 

y y L. y y d v* y ci l- 


tcctcacaat 


cagcgcact t 


7860 






nl" rscf^icrrat 

y L■^^cl.yclyv^u.^> 


taacaataaa 


tggcggagcc 


actcagggaa 


ccattcaggc 


7920 






Cl ^ ^ CI ^ W L-r U ^ CA 


w V--' CL C-^ ^ w W ^ 


aacccgaaga 


gatgattgta 


atgaaacgag 


7980 


§ 




n1" 1" 1" rrplrrt" a 

yL.L-^-ywi_yv-tA 


ri" QCl" CTrll" QCJ 


gctggtcggt 


aaatgcctgg 


tcattcgcct 


8040 


m 


y L-ciciciciL-L^yo 


V'Cici i_yy L-Ci^w 


CL u W w CL CL L. 


t cpict aocca 

L. CL C ^ u O 


tcattttaca 

C \^ C C C C CL CI 


acgt cgtgac 


8100 




yyy 


v^uyyv^y ^ ua.^ 


Vw* Vs> CL CL w l_ CL CL C 


COCCtt OCclCI 

^ M C C M CL y 


cacatccccc 


tt tcgccagc 


8160 




tggcgtaata 


gcgaagaggc 


ccgcaccgat 


cgcccttccc 


aacagttgcg 


cagcctgaat 


8220 


y I: 


ggcgaatggc 


gcctgatgcg 


gtattttctc 


cttacgcatc 


tgtgcggtat 


ttcacaccgc 


8280 




atatggtgca 


ctctcagtac 


aatctgctct 


gatgccgcat 


agttaagcca 


gccccgacac 


8340 




ccgccaacac 


ccgctgacgc 


gccctgacgg 


gcttgtctgc 


tcccggcatc 


cgcttacaga 


8400 




caagctgtga 


ccgtctccgg 


gagctgcatg 


tgtcagaggt 


tttcaccgtc 


atcaccgaaa 


8460 



cgcg 8464 



<210> 170 
<211> 27 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Synthetic M2 Peptide 
<400> 170 

Ser Leu Leu Thr Glu Val Glu Thr Pro He Arg Asn Glu Trp Gly Cys 
15 10 15 

Arg Cys Asn Gly Ser Ser Asp Gly Gly Gly Cys 
20 25 



<210> 171 



-91- 



<211> 97 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Matrix protein M2 
<400> 171 

Met Ser Leu Leu Thr Glu Val Glu Thr Pro lie Arg Asn Glu Trp Gly 
15 10 15 

Cys Arg Cys Asn Gly Ser Ser Asp Pro Leu Ala lie Ala Ala Asn lie 
20 25 30 

lie Gly lie Leu His Leu lie Leu Trp lie Leu Asp Arg Leu Phe Phe 
35 40 45 

Lys Cys lie Tyr Arg Arg PHe Lys Tyr Gly Leu Lys Gly Gly Pro Ser 
50 55 60 

Thr Glu Gly Val Pro Lys Ser Met Arg Glu Glu Tyr Arg Lys Glu Gin 
65 70 75 80 

Gin Ser Ala Val Asp Ala Asp Asp Gly His Phe Val Ser He Glu Leu 
85 90 95 

Glu 



<210> 172 

<211> 770 

<212> PRT 

<213> Homo Sapiens 

<400> 172 

Met Leu Pro Gly Leu Ala Leu Leu Leu Leu Ala Ala Trp Thr Ala Arg 
15 10 15 

Ala Leu Glu Val Pro Thr Asp Gly Asn Ala Gly Leu Leu Ala Glu Pro 
20 25 30 

Gin He Ala Met Phe Cys Gly Arg Leu Asn Met His Met Asn Val Gin 
35 40 45 

Asn Gly Lys Trp Asp Ser Asp Pro Ser Gly Thr Lys Thr Cys He Asp 
50 55 60 

Thr Lys Glu Gly He Leu Gin Tyr Cys Gin Glu Val Tyr Pro Glu Leu 
65 70 75 80 

Gin He Thr Asn Val Val Glu Ala Asn Gin Pro Val Thr He Gin Asn 
85 90 95 

Trp Cys Lys Arg Gly Arg Lys Gin Cys Lys Thr His Pro His Phe Val 
100 105 110 



-92- 



Ile Pro Tyr Arg Cys Leu Val Gly Glu Phe Val Ser Asp Ala Leu Leu 
115 120 125 

Val Pro Asp Lys Cys Lys Phe Leu His Gin Glu Arg Met Asp Val Cys 
130 135 140 

Glu Thr His Leu His Trp His Thr Val Ala Lys Glu Thr Cys Ser Glu 
145 150 155 160 

Lys Ser Thr Asn Leu His Asp Tyr Gly Met Leu Leu Pro Cys Gly lie 
165 170 175 

Asp Lys Phe Arg Gly Val Glu Phe Val Cys Cys Pro Leu Ala Glu Glu 
180 185 190 ■ 

Ser Asp Asn Val Asp Ser Ala Asp Ala Glu Glu Asp Asp Ser Asp Val 
195 200 205 

Trp Trp Gly Gly Ala Asp Thr Asp Tyr Ala Asp Gly Ser Glu Asp Lys 
210 215 220 

Val Val Glu Val Ala Glu Glu Glu Glu Val Ala Glu Val Glu Glu Glu 
225 230 235 240 

Glu Ala Asp Asp Asp Glu Asp Asp Glu Asp Gly Asp Glu Val Glu Glu 
245 250 255 

Glu Ala Glu Glu Pro Tyr Glu Glu Ala Thr Glu Arg Thr Thr Ser' He 
260 265 270 

Ala Thr Thr Thr Thr Thr Thr Thr Glu Ser Val Glu Glu Val Val Arg 
275 280 285 

Glu Val Cys Ser Glu Gin Ala Glu Thr Gly Pro Cys Arg Ala Met He 
290 295 300 

Ser Arg Trp Tyr Phe Asp Val Thr Glu Gly Lys Cys Ala Pro Phe Phe 
305 310 315 320 

Tyr Gly Gly Cys Gly Gly Asn Arg Asn Asn Phe Asp Thr Glu Glu Tyr 
325 330 335 

Cys Met Ala Val Cys Gly Ser Ala Met Ser Gin Ser Leu Leu Lys Thr 
340 345 350 

Thr Gin Glu Pro Leu Ala Arg Asp Pro Val Lys Leu Pro Thr Thr Ala 
355 360 365 

Ala Ser Thr Pro Asp Ala Val Asp Lys Tyr Leu Glu Thr Pro Gly Asp 
370 375 380 



Glu Asn Glu His Ala His Phe Gin Lys Ala Lys Glu Arg Leu Glu Ala 
385 390 395 400 

Lys His Arg Glu Arg Met Ser Gin Val Met Arg Glu Trp Glu Glu Ala 
405 410 415 

Glu Arg Gin Ala Lys Asn Leu Pro Lys Ala Asp Lys Lys Ala Val lie 
420 425 430 

Gin His Phe Gin Glu Lys Val Glu Ser Leu Glu Gin Glu Ala Ala Asn 
435 440 445 



-93- 



Glu Arg Gin Gin Leu Val Glu Thr His Met Ala Arg Val Glu Ala Met 
450 455 460 

Leu Asn Asp Arg Arg Arg Leu Ala Leu Glu Asn Tyr lie Thr Ala Leu 
465 470 475 480 

Gin Ala Val Pro Pro Arg Pro Arg His Val Phe Asn Met Leu Lys Lys 
485 490 495 

Tyr Val Arg Ala Glu Gin Lys Asp Arg Gin His Thr Leu Lys His Phe 
500 505 510 

Glu His Val Arg Met Val Asp Pro Lys Lys Ala Ala Gin He Arg Ser 
515 520 525 

Gin Val Met Thr His Leu Arg Val lie Tyr Glu Arg Met Asn Gin Ser 
530 535 540 

Leu Ser Leu Leu Tyr Asn Val Pro Ala Val Ala Glu Glu He Gin Asp 
545 550 555 560 

Glu Val Asp Glu Leu Leu Gin Lys Glu Gin Asn Tyr Ser Asp Asp Val 
565 570 575 

Leu Ala Asn Met He Ser Glu Pro Arg He Ser Tyr Gly Asn Asp Ala 
580 585 590 

Leu Met Pro Ser Leu Thr Glu Thr Lys Thr Thr Val Glu Leu Leu Pro 
595 600 605 

Val Asn Gly Glu Phe Ser Leu Asp Asp Leu Gin Pro Trp His Ser Phe 
610 615 620 

Gly Ala Asp Ser Val Pro Ala Asn Thr Glu Asn Glu Val Glu Pro Val 
625 630 635 640 

Asp Ala Arg Pro Ala Ala Asp Arg Gly Leu Thr Thr Arg Pro Gly Ser 
645 650 655 

Gly Leu Thr Asn He Lys Thr Glu Glu He Ser Glu Val Lys Met Asp 
660 665 670 

Ala Glu Phe Arg His Asp Ser Gly Tyr Glu Val His His Gin Lys Leu 
675 680 . 685 

Val Phe Phe Ala Glu Asp Val Gly Ser Asn Lys Gly Ala He He Gly 
690 695 700 

Leu Met Val Gly Gly Val Val He Ala Thr Val He Val He Thr Leu 
705 710 715 720 

Val Met Leu Lys Lys Lys Gin Tyr Thr Ser He His His Gly Val Val 
725 730 735 

Glu Val Asp Ala Ala Val Thr Pro Glu Glu Arg His Leu Ser Lys Met 
740 745 750 



Gin Gin Asn Gly Tyr Glu Asn Pro Thr Tyr Lys Phe Phe Glu Gin Met 
755 760 .765 



Gin Asn 
770 



-94- 



<210> 173 

<211> 82 

<212> PRT 

<213> Homo Sapiens 

<400> 173 

Gly Ser Gly Leu Thr Asn He Lys Thr Glu Glu He Ser Glu Val Lys 
15 10 15 

Met Asp Ala Glu Phe Arg His Asp Ser Gly Tyr Glu Val His His Gin 
20 25 30 

Lys Leu Val Phe Phe Ala Glu Asp Val Gly Ser Asn Lys Gly Ala He 
35 ■ 40 45 

He Gly Leu Met Val Gly Gly Val Val He Ala Thr Val He He He 
50 55 60 

Thr Leu Val Met Leu Lys Lys Gin Tyr Thr Ser Asn His Hi's Gly Val 
65 70 75 80 

Val Glu 



<210> 174 

<211> 42 

<212> PRT 

<213> Unknown 
<220> 

<223> Amyloid Beta Peptide 

<400> 174 

Asp Ala Glu Phe Arg His Asp Ser 
1 5 

Leu Val Phe Phe Ala Glu Asp Val 
20 

Gly Leu Met Val Gly Gly Val Val 
35 40 

<210> 175 

<211> 12 

<212> PRT 

<213> Artificial Sequence 



Gly Tyr Glu Val His His Gin Lys 
10 15 

Gly Ser Asn Lys Gly Ala He He 
25 30 

He Ala 



-95- 



<220> 

<223> p33 peptide 



ifi: 



<400> 175 

Cys Gly Gly Lys Ala Val Tyr Asn Phe Ala Thr Met 
1 5 10 

<210> 176 

<211> 37 

<212> PRT 

<213> Artificial Sequence 



<220> 

<223> DP178C peptide 
<400> 176 

Cys Tyr Thr Ser Leu lie His Ser Leu lie Glu Glu Ser Gin Asn Gin 
15 10 15 

Gin Glu Lys Asn Glu Gin Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser 
20 25 30 

Leu Trp Asn Trp Phe 
35 

<210> 177 
<211> 6 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> c-te'rminal linker 



<400> 177 

Gly Ser Gly Gly Cys Gly 
1 5 

<210> 178 

<211> 65 



-96- 

<212> PRT 

<213> Artificial Sequence 
<220> 

<223> GRA2 



<400> 178 

Lys Glu Ala Ala Gly Arg Gly Met Val Thr Val Gly Lys Lys Leu Ala 
15 10 15 

Asn Val Glu Ser Asp Arg Ser Thr Thr Thr Thr Gin Ala Pro Asp Ser 
20 25 30 

Pro Asn Gly Leu Ala Glu Thr Glu Val Pro Val Glu Pro Gin Gin Arg 
35 40 45 

Ala Ala His Val Pro Val Pro Asp Phe Ser Gin Gly Ser Gly Gly Cys 
50 55 . 60 

Gly 
65 

<210> 179 
<211> 18 
<212> PRT 

<213> Artificial Sequence 



<220> 

<223> D2 peptide 
<400> 179 

Cys Gly Gly Thr Ser Asn Gly Ser Asn Pro Ser Thr Ser Tyr Gly Phe 
15 10 15 

Ala Asn 

<210> 180 

<211> 18 

<212> PRT 

<213> Artificial Sequence 



-97- 



<220> 

<223> B2 peptide 
<400> 180 

Cys Gly Gly Asp lie Ser Asn Gly Tyr Gly Ala Ser Tyr Gly Asp Asn 
15 10 15 

Asp lie 

<210> 181 

<211> 14 

<212> PRT 

<213> Artificial Sequence 
<220> 

<223> muTNFa peptide 

<400> 181 

Cys Gly Gly Val Glu Glu Gin Leu Glu Trp Leu Ser Gin Arg 
1 5 10 

<210> 182 

<211> 22 

<212> PRT 

<213> Artificial Sequence 
<220> 

<223> TNFa II (3'-TNFa II) 
<400> 182 

Ser Ser Gin Asn Ser Ser Asp Lys Pro Val Ala His Val Val Ala Asn 
15 10 15 

His Gly Val Gly Gly Cys 
20 

<210> 183 
<211> 20 



-98- 

<212> PRT 

<213> Artificial Sequence 



<220> 

<223> TNFa II {5' TNFa II) 
<400> 183 



Cys Ser Ser Gin Asn Ser Ser Asp Lys Pro Val Ala His Val Val Ala 
^ 10 15 

Asn His Gly Val 
20 



<210> 184 
<211> 182 
<212> PRT 

<213> Escherichia coli 
<400> 184 

Met Lys lie Lys Thr Leu Ala He Val Val Leu Ser Ala Leu Ser Leu 



10 



15 



ser Ser Thr Ala Ala Leu Ala Ala Ala Thr Thr Val Asn Gly Gly 



25 



Thr 



30 



val His Phe Lys Gly Glu Val Val Asn Ala Ala Cys Ala Val Asp Ala 

. 40 45 

Gly ser val Asp Gin Thr Val Gin Leu Gly Gin Val Arg Thr Ala Ser 

55 60 

Leu Ala Gin Glu Gly Ala Thr Ser Ser Ala Val Gly Phe Asn He Gin 

75 80 

Leu Asn Asp Cys Asp Thr Asn Val Ala Ser Lys Ala Ala Val Ala Phe 



90 



95 



Leu Gly Thr Ala He Asp Ala Gly His Thr Asn Val Leu Ala Leu Gin 

100 105 no 

ser ser Ala Ala Gly Ser Ala Thr Asn Val Gly Val Gin He Leu Asp 
^ 120 ^ 125 

Arg Thr Gly Ala Ala Leu Thr Leu Asp Gly Ala Thr Phe Ser Ser Glu 

135 ^ 

Thr Thr Leu Asn Asn Gly Thr Asn Thr He Pro Phe Gin Ala Arg Tyr 



155 160 



Phe Ala Thr Gly Ma Ala Thr Pro Gly Ala Ala Asn Ala Asp Ala Thr 

^"^^ 175 

Phe Lys Val Gin Tyr Gin 
180 

<210> 185 
<211> 152 
<212> PRT 

<213> Hepatitis B virus 
<400> 185 

Met Asp lie ASP Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 

10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 

Zb 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala He Glu Ser Pro Glu His Cys 

ser Pro His His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Glu 

60 

Leu Met Thr Leu Ala Thr Trp Val Gly Thr Asn Leu Glu Asp Gly Gly 

'^5 90 

Lys Gly Gly Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met 

90 95 

Gly Leu Lys lie Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr 

105 

Phe Gly Arg Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp 

125 

lie Arg Thr Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser 

1J5 240 
Thr Leu Pro Glu Thr Thr Val Val 

<210> 186 
<211> 152 
<212> PRT 

<213> Hepatitis B virus 
<400> 186 

Met ASP lie Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
^ 10 15 



i 



-100- 

..U S„ .3p ™. ... ^ 

• 30 



™^ M, s„ M. .e„ r., ^^^^^ 

„,3 ™, 2^ 

60 ^ 
- M,. ™. 

Gly Leu Lys He Arg Gin Leu Leu 
100 

Phe Gly Arg Glu Thr Val Leu Glu 



s Ser 



Glu 



80 
Met 



Trp Phe His He Ser Ser Leu Thr 
110 



Tyr Leu Val Ser Phe Gly Val Trp 
125 

T.. P„ p„ 

ijo 140 
Thr Leu Pro Glu Thr Thr Val Val 
150