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SEQUENCE LISTING 



<110> Sebbel, Peter 

Dunant, Nicolas 
Bachmann, Martin 
Tissot, Alain 
Lechner, Franziska 

<120> Molecular Antigen Array 



<130> 1700.0180002 

<140> 
<141> 

<160> 186 

<170> Patentln Ver. 2.1 

<210> 1 
<211> 41 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 1 

ggggacgcgt gcagcaggta accaccgtta aagaaggcac c 

<210> 2 
<211> 44 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 2 

cggtggttac ctgctgcacg cgttgcttaa gcgacatgta gcgg 

<210> 3 
<211> 20 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 3 

ccatgaggcc tacgataccc 



<210> 4 
<211> 25 
<212> DNA 

<213> Artificial Sequence 



<220> 



<223> Primer 



<400> 4 

ggcactcacg gcgcgcttta caggc 25 



<210> 5 
<211> 47 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 5 

ccttctttaa cggtggttac ctgctggcaa ccaacgtggt tcatgac 47 



<210> 6 
<211> 40 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 6 

aagcatgctg cacgcgtgtg cggtggtcgg atcgcccggc 4 0 

<210> 7 
<211> 90 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 7 

gggtctagat tcccaaccat tcccttatcc aggctttttg acaacgctat gctccgcgcc 60 
catcgtctgc accagctggc ctttgacacc 90 



<210> 8 
<211> 108 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 8 

gggtctagaa ggaggtaaaa aacgatgaaa aagacagcta tcgcgattgc agtggcactg 60 
gctggtttcg ctaccgtagc gcaggccttc ccaaccattc ccttatcc 108 

<210> 9 
<211> 31 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



-3- 



<400> 9 

cccgaattcc tagaagccac agctgccctc c 



31 



<210> 10 
<211> 24 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 10 

cctgcggtgg tctgaccgac accc 24 



<210> 11 
<211> 41 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<210> 12 
<211> 33 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 12 

ctatcatcta gaatgaatag aggattcttt aac 33 



<210> 13 
<211> 15 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Modified ribosome 
binding site 



<210> 14 
<211> 21 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> signal peptide 



<400> 11 

ccgcggaaga gccaccgcaa ccaccgtgtg ccgccaggat g 



41 



<400> 13 

aggaggtaaa aaacg 



15 



<400> 14 

Met Lys Lys Thr Ala lie Ala lie Ala Val Ala Leu Ala Gly Phe Ala 



-4- 

15 10 15 

Thr Val Ala Gin Ala 
20 



<210> 15 
<211> 46 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> modified Fos 
construct 

<400> 15 

Cys Gly Gly Leu Thr Asp Thr Leu Gin Ala Glu Thr Asp Gin Val Glu 
15 10 15 

Asp Glu Lys Ser Ala Leu Gin Thr Glu He Ala Asn Leu Leu Lys Glu 
20 25 30 

Lys Glu Lys Leu Glu Phe He Leu Ala Ala His Gly Gly Cys 
35 40 45 



<210> 16 
<211> 6 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> peptide linker 
<400> 16 

Ala Ala Ala Ser Gly Gly 
1 5 



<210> 17 
<211> 6 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> peptide linker 
<400> 17 

Gly Gly Ser Ala Ala Ala 
1 5 



<210> 18 
<211> 256 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Fos fusion construct 
<400> 18 

gaattcagga ggtaaaaaac gatgaaaaag acagctatcg cgattgcagt ggcactggct 60 
ggtttcgcta ccgtagcgca ggcctgggtg ggggcggccg cttctggtgg ttgcggtggt 120 



ctgaccgaca ccctgcaggc ggaaaccgac caggtggaag acgaaaaatc cgcgctgcaa 180 
accgaaatcg cgaacctgct gaaagaaaaa gaaaagctgg agttcatcct ggcggcacac 24 0 
ggtggttgct aagctt 256 



<210> 19 
<211> 52 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Fos fusion construct 
<400> 19 

Ala Ala Ala Ser Gly Gly Cys Gly Gly Leu Thr Asp Thr Leu Gin Ala 



Glu Thr Asp Gin Val Glu Asp Glu Lys Ser Ala Leu Gin Thr Glu lie 



Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe lie Leu Ala Ala 
35 40 45 



His Gly Gly Cys 



<210> 20 
<211> 261 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<220> 

<221> CDS 

<222> (22) . . (240) 



gaattcagga ggtaaaaaac g atg aaa aag aca get ate gcg att gca gtg 51 
Met Lys Lys Thr Ala lie Ala lie Ala Val 
1 5 10 

gca ctg get ggt ttc get acc gta gcg cag gec tgc ggt ggt ctg acc 99 
Ala Leu Ala Gly Phe Ala Thr Val Ala Gin Ala Cys Gly Gly Leu Thr 
15 20 25 

gac acc ctg cag gcg gaa acc gac cag gtg gaa gac gaa aaa tec gcg 147 
Asp Thr Leu Gin Ala Glu Thr Asp Gin Val Glu Asp Glu Lys Ser Ala 
30 35 40 

ctg caa acc gaa ate gcg aac ctg ctg aaa gaa aaa gaa aag ctg gag 195 
Leu Gin Thr Glu lie Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu 
45 50 55 

ttc ate ctg gcg gca cac ggt ggt tgc ggt ggt tct gcg gec get 240 
Phe He Leu Ala Ala His Gly Gly Cys Gly Gly Ser Ala Ala Ala 



gggtgtgggg atatcaagct t 



261 



<210> 21 
<211> 73 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<400> 21 

Met Lys Lys Thr Ala lie Ala lie 

1 5 

Thr Val Ala Gin Ala Cys Gly Gly 
20 

Thr Asp Gin Val Glu Asp Glu Lys 

35 40 

Asn Leu Leu Lys Glu Lys Glu Lys 
50 55 

Gly Gly Cys Gly Gly Ser Ala Ala 
65 70 



Ala Val Ala Leu Ala Gly Phe Ala 
10 15 

Leu Thr Asp Thr Leu Gin Ala Glu 
25 30 

Ser Ala Leu Gin Thr Glu lie Ala 
45 



Leu Glu Phe He Leu Ala Ala His 
60 



Ala 



<210> 22 
<211> 196 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<220> 

<221> CDS 

<222> (34) . . (189) 

<400> 22 

gaattcagga ggtaaaaaga tatcgggtgt ggg gcg gcc get tct ggt ggt tgc 54 

Ala Ala Ala Ser Gly Gly Cys 
1 5 

ggt ggt ctg acc gac acc ctg cag gcg gaa acc gac cag gtg gaa gac 102 
Gly Gly Leu Thr Asp Thr Leu Gin Ala Glu Thr Asp Gin Val Glu Asp 
10 15 20 

gaa aaa tec gcg ctg caa acc gaa ate gcg aac ctg ctg aaa gaa aaa 150 
Glu Lys Ser Ala Leu Gin Thr Glu He Ala Asn Leu Leu Lys Glu Lys 
25 30 35 

gaa aag ctg gag ttc ate ctg gcg gca cac ggt ggt tgc taagctt 196 
Glu Lys Leu Glu Phe He Leu Ala Ala His Gly Gly Cys 
40 45 50 



<210> 23 
<211> 52 
<212> PRT 

<213> Artificial Sequence 



<220> 

<223> Fos fusion 
construct 



<400> 23 

Ala Ala Ala Ser Gly Gly Cys Gly Gly Leu Thr Asp Thr Leu Gin Ala 
15 10 15 

Glu Thr Asp Gin Val Glu Asp Glu Lys Ser Ala Leu Gin Thr Glu He 
20 25 30 

Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe He Leu Ala Ala 
35 40 45 

His Gly Gly Cys 
50 



<210> 24 
<211> 204 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<400> 24 

gaattcagga ggtaaaaaac gatggcttgc 
accgaccagg tggaagacga aaaatccgcg 
gaaaaagaaa agctggagtt catcctggcg 
gctgggtgtg gggatatcaa gctt 



ggtggtctga ccgacaccct gcaggcggaa 60 
ctgcaaaccg aaatcgcgaa cctgctgaaa 120 
gcacacggtg gttgcggtgg ttctgcggcc 180 
204 



<210> 25 
<211> 56 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<400> 25 

Lys Thr Met Ala Cys Gly Gly Leu Thr Asp Thr Leu Gin Ala Glu Thr 

15 10 15 

Asp Gin Val Glu Asp Glu Lys Ser Ala Leu Gin Thr Glu He Ala Asn 

20 25 30 

Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe He Leu Ala Ala His Gly 

35 40 45 

Gly Cys Gly Gly Ser Ala Ala Ala 
50 55 



<210> 26 
<211> 26 
<212> PRT 

<213> Homo sapiens 
<400> 26 

Met Ala Thr Gly Ser Arg Thr Ser Leu Leu Leu Ala Phe Gly Leu Leu 
15 10 15 



Cys Leu Pro Trp Leu Gin Glu Gly Ser Ala 
20 25 



<210> 27 
<211> 262 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<400> 27 

gaattcaggc ctatggctac aggctcccgg acgtccctgc tcctggcttt tggcctgctc 60 

tgcctgccct ggcttcaaga gggcagcgct gggtgtgggg cggccgcttc tggtggttgc 120 

ggtggtctga ccgacaccct gcaggcggaa accgaccagg tggaagacga aaaatccgcg 180 

ctgcaaaccg aaatcgcgaa cctgctgaaa gaaaaagaaa agctggagtt catcctggcg 240 
gcacacggtg gttgctaagc tt 262 



<210> 28 
<211> 52 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<400> 28 

Ala Ala Ala Ser Gly Gly Cys Gly 
5 

Glu Thr Asp Gin Val Glu Asp Glu 
20 

Ala Asn Leu Leu Lys Glu Lys Glu 
35 40 

His Gly Gly Cys 
50 



Gly Leu Thr Asp Thr Leu Gin Ala 
10 15 

Lys Ser Ala Leu Gin Thr Glu lie 
25 30 

Lys Leu Glu Phe lie Leu Ala Ala 
45 



<210> 29 
<211> 261 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<220> 

<221> CDS 

<222> (7) . . (240) 

<400> 29 

gaattc atg get aca ggc tec egg acg tec ctg etc ctg get ttt ggc 48 

Met Ala Thr Gly Ser Arg Thr Ser Leu Leu Leu Ala Phe Gly 
15 10 



ctg etc tgc ctg ccc tgg ctt caa gag ggc age get tgc ggt ggt ctg 96 
Leu Leu Cys Leu Pro Trp Leu Gin Glu Gly Ser Ala Cys Gly Gly Leu 
15 20 25 30 



acc gac acc ctg cag gcg gaa acc gac cag gtg gaa gac gaa aaa tec 
Thr Asp Thr Leu Gin Ala Glu Thr Asp Gin Val Glu Asp Glu Lys Ser 



gcg ctg caa acc gaa ate gcg aac ctg ctg aaa gaa aaa gaa aag ctg 
Ala Leu Gin Thr Glu lie Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu 



gag ttc ate ctg gcg gca cac ggt ggt tgc ggt ggt tct gcg gec get 
Glu Phe He Leu Ala Ala His Gly Gly Cys Gly Gly Ser Ala Ala Ala 



gggtgtggga ggectaaget t 



<210> 30 
<211> 78 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 

<400> 30 

Met Ala Thr Gly Ser Arg Thr Ser 
1 5 

Cys Leu Pro Trp Leu Gin Glu Gly 
20 

Thr Leu Gin Ala Glu Thr Asp Gin 

35 40 

Gin Thr Glu He Ala Asn Leu Leu 
50 55 



He Leu Ala Ala His Gly Gly Cys 
65 70 



Leu Leu Leu Ala Phe Gly Leu Leu 
10 15 

Ser Ala Cys Gly Gly Leu Thr Asp 
25 30 

Val Glu Asp Glu Lys Ser Ala Leu 
45 

Lys Glu Lys Glu Lys Leu Glu Phe 
60 

Gly Gly Ser Ala Ala Ala 
75 



<210> 31 
<211> 44 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 31 

cctgggtggg ggcggccgct tctggtggtt gcggtggtct 



<210> 32 
<211> 44 
<212> DNA 

<213> Artificial Sequence 



-10- 



<220> 

<223> Primer 
<400> 32 

ggtgggaatt caggaggtaa aaagatatcg ggtgtggggc ggcc 44 



<210> 33 
<211> 47 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 33 

ggtgggaatt caggaggtaa aaaacgatgg cttgcggtgg tctgacc 47 



<210> 34 
<211> 18 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 34 

gcttgcggtg gtctgacc 18 



<210> 35 
<211> 27 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 35 

ccaccaagct tagcaaccac cgtgtgc 27 



<210> 36 
<211> 54 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 36 

ccaccaagct tgatatcccc acacccagcg gccgcagaac caccgcaacc accg 54 



<210> 37 
<211> 32 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



-11- 



<400> 37 

ccaccaagct taggcctccc acacccagcg gc 



<210> 38 
<211> 29 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 38 

ggtgggaatt caggaggtaa aaaacgatg 



<210> 39 
<211> 32 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 39 

ggtgggaatt caggcctatg gctacaggct cc 



<210> 40 
<211> 27 
<212> DNA 



29 



<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 40 

ggtgggaatt catggctaca ggctccc 27 



<210> 41 
<211> 59 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 41 

gggtctagaa tggctacagg ctcccggacg tccctgctcc tggcttttgg cctgctctg 59 

<210> 42 
<211> 58 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 42 

cgcaggcctc ggcactgccc tcttgaagcc agggcaggca gagcaggcca aaagccag 58 



-12- 



<210> 43 
<211> 402 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Modified bee 

venom phospholipase A2 

<220> 

<221> CDS 

<222> (1) . . (402) 

<400> 43 

ate ate tac cca ggt act ctg tgg tgt ggt cac ggc aac aaa tct tct 48 
lie lie Tyr Pro Gly Thr Leu Trp Cys Gly His Gly Asn Lys Ser Ser 
15 10 15 

ggt ccg aac gaa etc ggc cgc ttt aaa cac acc gac gca tgc tgt cgc 96 
Gly Pro Asn Glu Leu Gly Arg Phe Lys His Thr Asp Ala Cys Cys Arg 
20 25 30 

acc cag gac atg tgt ccg gac gtc atg tct get ggt gaa tct aaa cac 14 4 
Thr Gin Asp Met Cys Pro Asp Val Met Ser Ala Gly Glu Ser Lys His 
35 40 45 

ggg tta act aac acc get tct cac acg cgt etc age tgc gac tgc gac 192 
Gly Leu Thr Asn Thr Ala Ser His Thr Arg Leu Ser Cys Asp Cys Asp 
50 55 60 

gac aaa ttc tac gac tgc ctt aag aac tec gee gat acc ate tct tct 240 
Asp Lys Phe Tyr Asp Cys Leu Lys Asn Ser Ala Asp Thr lie Ser Ser 
65 70 75 80 

tac ttc gtt ggt aaa atg tat ttc aac ctg ate gat acc aaa tgt tac 288 
Tyr Phe Val Gly Lys Met Tyr Phe Asn Leu lie Asp Thr Lys Cys Tyr 
85 90 95 

aaa ctg gaa cac ccg gta acc ggc tgc ggc gaa cgt acc gaa ggt cgc 336 
Lys Leu Glu His Pro Val Thr Gly Cys Gly Glu Arg Thr Glu Gly Arg 
100 105 110 

tgc ctg cac tac acc gtt gac aaa tct aaa ccg aaa gtt tac cag tgg 384 
Cys Leu His Tyr Thr Val Asp Lys Ser Lys Pro Lys Val Tyr Gin Trp 
115 120 125 

ttc gac ctg cgc aaa tac 402 
Phe Asp Leu Arg Lys Tyr 
130 



<210> 44 
<211> 134 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Modified bee 

venom phospholipase A2 

<400> 44 

lie lie Tyr Pro Gly Thr Leu Trp Cys Gly His Gly Asn Lys Ser Ser 
15 10 15 



-13- 



Gly Pro Asn Glu Leu Gly Arg Phe Lys His Thr Asp Ala Cys Cys Arg 
20 25 30 

Thr Gin Asp Met Cys Pro Asp Val Met Ser Ala Gly Glu Ser Lys His 
35 40 45 

Gly Leu Thr Asn Thr Ala Ser His Thr Arg Leu Ser Cys Asp Cys Asp 



Asp Lys Phe Tyr Asp Cys Leu Lys Asn Ser Ala Asp Thr lie Ser Ser 
65 70 75 80 

Tyr Phe Val Gly Lys Met Tyr Phe Asn Leu lie Asp Thr Lys Cys Tyr 
85 90 95 

Lys Leu Glu His Pro Val Thr Gly Cys Gly Glu Arg Thr Glu Gly Arg 
100 105 110 

Cys Leu His Tyr Thr Val Asp Lys Ser Lys Pro Lys Val Tyr Gin Trp 
115 120 125 

Phe Asp Leu Arg Lys Tyr 
130 



<210> 45 
<211> 19 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 45 

ccatcatcta cccaggtac 



<210> 46 
<211> 34 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 46 

cccacaccca gcggccgcgt atttgcgcag gtcg 



<210> 47 
<211> 36 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 47 

cggtggttct gcggccgcta tcatctaccc aggtac 



<210> 48 



-14- 



<211> 19 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 48 

ttagtatttg cgcaggtcg 



<210> 49 
<211> 18 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 49 

ccggctccat cggtgcag 



<210> 50 
<211> 36 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 50 

accaccagaa gcggccgcag gggaaacaca tctgcc 



<210> 51 
<211> 35 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 51 

cggtggttct gcggccgctg gctccatcgg tgcag 



<210> 52 
<211> 21 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 52 

ttaaggggaa acacatctgc c 



<210> 53 
<211> 33 
<212> DNA 

<213> Artificial Sequence 



-15- 



<220> 

<223> Primer 
<4 00> 53 

actagtctag aatgagagtg aaggagaaat ate 



<210> 54 
<211> 42 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 54 

tagcatgeta gcaccgaatt tatctaattc caataattct tg 



<210> 55 
<211> 51 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 55 

gtagcaccca ccaaggcaaa gctgaaagct acccagctcg agaaactggc 



<210> 56 
<211> 48 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 56 

caaagctcct attcccactg ccagtttctc gagctgggta gctttcag 



<210> 57 
<211> 36 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 57 

ttcggtgcta gcggtggctg cggtggtctg accgac 



<210> 58 
<211> 37 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<400> 58 

gatgctgggc ccttaaccgc aaccaccgtg tgccgcc 



<210> 59 
<211> 46 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> JUN amino acid 
sequence 

<400> 59 

Cys Gly Gly Arg lie Ala Arg Leu 
1 5 

Ala Gin Asn Ser Glu Leu Ala Ser 
20 

Val Ala Gin Leu Lys Gin Lys Val 

35 40 



Glu Glu Lys Val Lys Thr Leu Lys 
10 15 

Thr Ala Asn Met Leu Arg Glu Gin 
25 30 

Met Asn His Val Gly Cys 
45 



<210> 60 
<211> 46 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> FOS amino 

acid sequence 

<400> 60 

Cys Gly Gly Leu Thr Asp Thr Leu Gin Ala Glu Thr Asp Gin Val Glu 

15 10 15 

Asp Glu Lys Ser Ala Leu Gin Thr Glu lie Ala Asn Leu Leu Lys Glu 
20 25 30 

Lys Glu Lys Leu Glu Phe lie Leu Ala Ala His Gly Gly Cys 
35 40 45 



<210> 61 
<211> 33 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 61 

ccggaattca tgtgcggtgg tcggatcgcc egg 



<210> 62 
<211> 39 
<212> DNA 

<213> Artificial Sequence 



<220> 

<223> Primer 



-17- 



<4 00> 62 

gtcgctaccc gcggctccgc aaccaacgtg gttcatgac 



39 



<210> 63 
<211> 50 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 63 

gttggttgcg gagccgcggg tagcgacatt gacccttata aagaatttgg 50 



<210> 64 
<211> 38 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<210> 65 
<211> 33 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 65 

ctagccgcgg gttgcggtgg tcggatcgcc egg 33 



<210> 66 
<211> 38 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<210> 67 
<211> 31 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 67 

ceggaattea tggacattga cccttataaa g 31 



<400> 64 

cgcgtcccaa gettctaegg aagcgttgat aggatagg 



38 



<400> 66 

cgcgtcccaa gcttttagca accaacgtgg ttcatgac 



38 



-18- 

<210> 68 
<211> 45 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 68 

ccgaccaccg caacccgcgg ctagcggaag cgttgatagg atagg 45 



<210> 69 
<211> 47 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 69 

ctaatggatc cggtgggggc tgcggtggtc ggatcgcccg gctcgag 47 



<210> 70 
<211> 39 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 70 

gtcgctaccc gcggctccgc aaccaacgtg gttcatgac 39 



<210> 71 
<211> 31 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 71 

ccggaattca tggacattga cccttataaa g 



<210> 72 
<211> 48 
<212> DNA 



31 



<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 72 

ccgaccaccg cagcccccac cggatccatt agtacccacc caggtagc 48 



<210> 73 
<211> 45 
<212> DNA 



-19- 



<213> Artificial Sequence 



<220> 

<223> Primer 



<400> 73 

gttggttgcg gagccgcggg tagcgaccta gtagtcagtt atgtc 



45 



<210> 74 
<211> 38 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 74 

cgcgtcccaa gcttctacgg aagcgttgat aggatagg 38 



<210> 75 
<211> 33 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<210> 76 
<211> 38 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 76 

cgcgtcccaa gcttttagca accaacgtgg ttcatgac 38 



<210> 77 
<211> 30 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<400> 75 

ctagccgcgg gttgcggtgg tcggatcgcc egg 



33 



<400> 77 

ceggaattea tggccacact tttaaggagc 



30 



<210> 78 
<211> 38 
<212> DNA 



<213> Artificial Sequence 



<220> 



-20- 



<223> Primer 
<400> 78 

cgcgtcccaa gcttttagca accaacgtgg ttcatgac 



<210> 79 
<211> 31 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<4O0> 79 

ccggaattca tggacattga cccttataaa g 



<210> 80 
<211> 51 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 80 

cctagagcca cctttgccac catcttctaa attagtaccc acccaggtag 



<210> 81 
<211> 48 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 81 

gaagatggtg gcaaaggtgg ctctagggac ctagtagtca gttatgtc 



<210> 82 
<211> 38 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 82 

cgcgtcccaa gcttctaaac aacagtagtc tccggaag 



<210> 83 
<211> 36 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<400> 83 



-21- 



gccgaattcc tagcagctag caccgaattt atctaa 



36 



<210> 84 
<211> 33 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 84 

ggttaagtcg acatgagagt gaaggagaaa tat 33 



<210> 85 
<211> 30 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<210> 86 
<211> 35 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 
<400> 86 

gaagtaaagc ttttaaccac cgcaaccacc agaag 35 



<210> 87 
<211> 33 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Primer 



<210> 88 
<211> 4 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Fos fusion 
construct 



<400> 85 

taaccgaatt caggaggtaa aaagatatgg 



30 



<400> 87 

tcgaatgggc cctcatcttc gtgtgctagt cag 



33 



<400> 88 
Glu Phe Arg Arg 
1 



-22- 



<210> 89 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 89 

Met Asp lie Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 



Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Glu 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro lie 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Thr Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 



Gin Ser Arg Gly Ser Gin Cys 
180 



<210> 90 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 90 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu 
50 55 60 



Leu Met Thr Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro Thr 



-23- 



65 



Ser Arg Asp Leu 



Phe Arg Gin Leu 
100 

Glu Thr Val lie 
115 

Pro Pro Ala Tyr 
130 



Glu Thr Cys Val 
145 



Pro Ser Pro Arg 



Gin Ser Arg Gly 
180 



Val Val Ser Tyr 
85 

Leu Trp Phe His 



Glu Tyr Leu Val 
120 

Arg Pro Thr Asn 
135 



lie Arg Arg Arg 
150 



Arg Arg Arg Ser 
165 

Ser Gin Cys 



75 

Val Asn Thr Asn 
90 

lie Ser Cys Leu 
105 

Ser Phe Gly Val 



Ala Pro lie Leu 
140 



Gly Arg Ser Pro 
155 



Gin Ser Pro Arg 
170 



80 

Met Gly Leu Lys 
95 

Thr Phe Gly Arg 

110 

Trp lie Arg Thr 
125 



Ser Thr Leu Pro 



Arg Arg Arg Thr 
160 



Arg Arg Arg Ser 
175 



<210> 91 
<211> 212 
<212> PRT 
<213> Hepatitis 

<400> 91 

Met Gin Leu Phe 



Val Gin Ala Ser 
20 

Asp Pro Tyr Lys 

35 

Pro Ser Asp Phe 
50 

Ala Leu Tyr Arg 
65 

His Thr Ala Leu 



Leu Ala Thr Trp 
100 

Leu Val Val Ser 
115 

Leu Leu Trp Phe 
130 

lie Glu Tyr Leu 
145 



B virus 



His Leu Cys Leu 
5 

Lys Leu Cys Leu 



Glu Phe Gly Ala 
40 

Phe Pro Ser Val 
55 

Glu Ala Leu Glu 
70 

Arg Gin Ala lie 
85 

Val Gly Gly Asn 



Tyr Val Asn Thr 
120 

His lie Ser Cys 
135 

Val Ser Phe Gly 
150 



lie lie Ser Cys 
10 

Gly Trp Leu Trp 
25 

Thr Val Glu Leu 



Arg Asp Leu Leu 
60 

Ser Pro Glu His 
75 

Leu Cys Trp Gly 
90 

Leu Glu Asp Pro 
105 

Asn Met Gly Leu 



Leu Thr Phe Gly 

140 

Val Trp lie Arg 
155 



Ser Cys Pro Thr 
15 

Gly Met Asp He 
30 

Leu Ser Phe Leu 

45 

Asp Thr Ala Ser 



Cys Ser Pro His 
80 

Glu Leu Met Thr 
95 

He Ser Arg Asp 
110 



Lys Phe Arg Gin 
125 

Arg Glu Thr Val 



Thr Pro Pro Ala 
160 



Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 



-24- 



Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 92 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 92 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 



Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Asn Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro lie Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

lie Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 93 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 



-25- 



<400> 93 

Met Asp lie Asp Pro Tyr Lys 



Ser Phe Leu Pro Thr Asp Phe Phe Pro Ser Val 
20 25 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu 



35 



Ser Pro His His Thr Ala Leu 
50 55 



Leu Met Thr Leu Ala Thr Trp 
65 70 

Ser Arg Asp Leu Val Val Ser 



Phe Arg Gin Leu Leu Trp Phe 
100 



Glu Thr Val lie Glu Tyr Leu 
115 



Glu Phe Gly Ala Thr Val Glu Leu Leu 
10 15 

Leu Asp 
His Cys 
Gly Glu 



40 



Arg Asp Leu 
30 



Ser Pro Glu 
45 



Arg Gin Ala lie 

,Val Gly Val Asn 
75 

Tyr Val Asn Thr 
90 

Ser Cys 

Phe Gly 



Leu Cys Trp 
60 



80 



Leu Glu Asp Pro Ala 
Asn Met Gly 



His He 
105 



Val Se 
120 



Leu Thr Phe 
110 



Val Trp He 
125 



Leu Lys 
95 

Gly Arg 
Arg Thr 



Pro Pro Ala Tyr Arg Pro Pro 
130 135 



Asn Ala Pro He 



Leu Ser Thr 
140 



Leu Pro 



Glu Thr Cys Val Val Arg Arg . 
145 150 



Pro Ser Pro Arg Arg Arg Arg 
165 



Ser Pro 
170 



Pro Arg Arg 
Arg Arg Arg 



Arg Thr 
160 



Arg Ser 
175 



Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 94 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 94 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro Val Ser Arg Asp 



100 



105 



110 



Leu Val Val Ser Tyr Val Asn Thr 
115 120 



Leu Leu Trp Phe His lie Ser Cys 
130 135 



He Glu Tyr Leu Val Ser Phe Gly 
145 150 



Tyr Arg Pro Pro Asn Ala Pro He 
165 



Val Val Arg Arg Arg Gly Arg Ser 
180 



Arg Arg Arg Arg Ser Gin Ser Pro 
195 200 



Glu Ser Gin Cys 
210 



Asn Val Gly Leu Lys Phe Arg Gin 
125 



Leu Thr Phe Gly Arg Glu Thr Val 
140 



Val Trp He Arg Thr Pro Pro Ala 
155 160 



Leu Ser Thr Leu Pro Glu Thr Thr 
170 175 



Pro Arg Arg Arg Thr Pro Ser Pro 
185 190 



Arg Arg Arg Arg Ser Gin Ser Arg 
205 



<210> 95 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 95 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Asp Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 



His Thr Ala Leu Arg Gin Ala He Leu Cvs Trp Gly Asp Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro Val Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Val Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 



-27- 



Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 96 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 96 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro Gin 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Glu Leu Met Thr 



Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro lie Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

lie Glu Tyr Leu Val Ser Phe Gly Val Trp lie Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 



Glu Ser Gin Cys 
210 



<210> 97 
<211> 212 
<212> PRT 
<213> Hepatitis 



<4 00> 97 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 
15 10 15 



Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 



Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 



Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 



Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Lys Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Gly Ser Gin Cys 
210 



<210> 98 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 98 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Phe Arg Asp Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu 
50 55 60 



Leu Met Thr Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro Ala 



-29- 



65 



Ser Arg Asp Leu 



Phe Arg Gin Leu 

100 

Asp Thr Val lie 
115 



Pro Pro Ala Tyr 
130 



Glu Thr Cys Val 
145 

Pro Ser Pro Arg 



Gin Ser Arg Glu 
180 



70 

Val Val Ser Tyr 
85 

Leu Trp Phe His 



Glu Tyr Leu Val 

120 



Arg Pro Ser Asn 
135 



Val Arg Arg Arg 
150 



Arg Arg Arg Ser 

165 



Ser Gin Cys 



75 

Val Asn Thr Asn 
90 

lie Ser Cys Leu 
105 

Ser Phe Gly Val 



Ala Pro He Leu 

140 

Gly Arg Ser Pro 
155 

Gin Ser Pro Arg 
170 



80 

Met Gly Leu Lys 
95 

Thr Phe Gly Arg 
110 

Trp He Arg Thr 

125 

Ser Thr Leu Pro 



Arg Arg Arg Thr 
160 

Arg Arg Arg Ser 
175 



<210> 99 
<211> 183 
<212> PRT 
<213> Hepatitis 

<400> 99 
Met Asp He Asp 
1 

Ser Phe Leu Pro 
20 

Thr Ala Ser Ala 
35 

Ser Pro His His 

50 

Leu Met Thr Leu 
65 

Ser Arg Asp Leu 



Phe Arg Gin Leu 

100 

Glu Thr Val He 
115 

Pro Pro Ala Tyr 
130 

Glu Thr Thr Val 
145 



B virus 



Pro Tyr Lys Glu 
5 

Ser Asp Phe Phe 



Leu Tyr Arg Glu 
40 

Thr Ala Leu Arg 
55 

Ala Thr Trp Val 
70 

Val Val Ser Tyr 
85 

Leu Trp Phe His 



Glu Tyr Leu Val 
120 

Arg Pro Pro Asn 
135 

Val Arg Arg Arg 
150 



Phe Gly Ala Thr 
10 

Pro Ser Val Arg 
25 

Ala Leu Glu Ser 



Gin Ala He Leu 
60 

Gly Val Asn Leu 
75 

Val Asn Thr Asn 
90 

He Ser Cys Leu 
105 

Ser Phe Gly Val 



Ala Pro He Leu 
140 



Gly Arg Ser Pro 
155 



Val Glu Leu Leu 
15 

Asp Leu Leu Asp 
30 

Pro Glu His Cys 
45 

Cys Trp Gly Glu 



Glu Asp Pro Ala 
80 



Met Gly Leu Lys 
95 



Thr Phe Gly Arg 
110 

Trp He Arg Thr 
125 

Ser Thr Leu Pro 



Arg Arg Arg Thr 
160 



Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 



-30- 



Gln Ser Arg Glu Ser Gin Cys 
180 



<210> 100 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 



<400> 100 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 



Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg His Ala lie Leu Cys Trp Gly Asp Leu Arg Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro lie Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

lie Glu Tyr Leu Val Ser Phe Gly Val Trp lie Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 



Glu Ser Gin Cys 
210 



<210> 101 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 



<400> 101 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 



Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Asp Met Asp lie 



-31- 



20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 



Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 

50 55 60 

Ala Leu Phe Arg Asp Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 

65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 



Leu Ala Thr Trp Val Gly Ala Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Gin Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Cys 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 102 
<211> 183 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> synthetic 

human Hepatitus B construct 

<400> 102 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu 



Leu Met Thr Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala 
65 70 75 80 



-32- 



Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp lie Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Thr Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 103 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 103 

Met Gin Leu Phe His 

1 5 

Val Gin Ala Ser Lys 
20 

Asp Pro Tyr Lys Glu 
35 

Pro Ser Asp Phe Phe 
50 

Ala Leu Tyr Arg Glu 
65 

His Thr Ala Leu Arg 
85 

Leu Ala Thr Trp Val 
100 

Leu Val Val Ser Tyr 
115 

Leu Leu Trp Phe His 
130 

lie Glu Tyr Leu Val 
145 

Tyr Arg Pro Pro Asn 
165 



Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 
10 15 

Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 

25 30 

Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
40 45 

Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
55 60 

Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 



Gin Ala lie Leu Cys Trp Gly Asp Leu Met Ser 
90 95 

Gly Val Asn Leu Glu Asp Pro lie Ser Arg Asp 
105 110 

Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
120 125 

lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
135 140 

Ser Phe Gly Val Trp lie Arg Thr Pro Pro Ala 
150 155 160 

Ala Pro lie Leu Ser Thr Leu Pro Glu Thr Thr 
170 175 



Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 



-33- 



Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 



Glu Ser Gin Cys 
210 



<210> 104 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 104 

Met Asp lie Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 



Thr Ala Ser Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Glu 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 

130 135 140 

Glu Thr Thr Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 



Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 105 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 105 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 



-34- 



35 

Ser Pro His His 
50 

Leu Met Thr Leu 
65 

Ser Arg Asp Leu 



Phe Arg Gin Leu 
100 

Glu Thr Val He 
115 

Pro Pro Ala Tyr 
130 

Glu Thr Thr Val 
145 

Pro Ser Pro Arg 



Gin Ser Arg Glu 
180 



40 



Thr Ala Leu Arg 
55 

Ala Thr Trp Val 
70 

Val Val Ser Tyr 
85 

Leu Trp Phe His 



Glu Tyr Leu Val 
120 



Arg Pro Pro Asn 

135 

Val Arg Arg Arg 
150 

Arg Arg Arg Ser 
165 

Ser Gin Cys 



Gin Ala He Leu 
60 

Gly Val Asn Leu 
75 

Val Asn Thr Asn 
90 

He Ser Cys Leu 
105 

Ser Phe Gly Val 



Ala Pro He Leu 
140 

Gly Arg Ser Pro 
155 

Gin Ser Pro Arg 
170 



45 

Cys Trp Gly Asp 



Glu Asp Pro Ala 
80 

Met Gly Leu Lys 
95 

Thr Phe Gly Arg 
110 



Trp He Arg Thr 
125 

Ser Thr Leu Pro 



Arg Arg Arg Thr 
160 



Arg Arg Arg Ser 
175 



<210> 106 
<211> 183 
<212> PRT 
<213> Hepatitis 

<400> 106 
Met Asp He Asp 



Pro Tyr Lys 

5 10 

Ser Asp Phe Phe Pro Ser Val 
Thr Ala Ser Ala Leu Tyr Arg 



Ser Phe Leu Pro 
20 



Glu Phe Gly Ala Thr Val 
Arg Asp 



25 



Asp Ala Leu Glu Ser Pro 

35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys 

50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Ala Asn Leu Glu 

65 70 75 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met 



Phe Arg Gin Leu 
100 



Glu Thr Val He 
115 



85 

Leu Trp Phe 
Glu Tyr Leu 



His He 
105 



Val Ser 
120 



90 

Ser Cys 
Phe Gly 



Glu Leu Leu 

15 

Leu Leu Asp 
30 

Glu His Cys 

Trp Gly Glu 

Asp Pro Ala 
80 

Gly Leu Lys 
95 

Phe Gly Arg 
110 



Val Trp 
125 



Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 



-35- 



Glu Thr Thr Val Val Arg Arg Arg Gly Arg Thr Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 107 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 107 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp'Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 



Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 



Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 108 
<211> 212 
<212> PRT 
<213> Hepatitis 



-36- 



<400> 108 

Met Gin Leu Phe His 
1 5 

Val Gin Ala Ser Lys 
20 

Asp Pro Tyr Lys Glu 
35 

Pro Ser Asp Phe Phe 
50 

Ala Leu Tyr Arg Glu 
65 

His Thr Ala Leu Arg 

85 



Leu Cys Leu lie lie Ser Cys 
10 

Leu Cys Leu Gly Trp Leu Trp 
25 

Phe Gly Ala Thr Val Glu Leu 
40 

Pro Ser Val Arg Asp Leu Leu 
55 60 

Ala Leu Glu Ser Pro Glu His 
70 75 

Gin Ala lie Leu Cys Trp Gly 
90 



15 



Ser Cys Pro Thr 
Gly Met . 



30 



Asp lie 

Leu Ser Phe Leu 
45 

Asp Thr Ala Ser 

Cys Ser Pro His 
80 

Asp Leu Met Thr 
95 



Leu Ala Thr Trp Val 
100 



Leu Val Val Ser Tyr 
115 



Gly Val Asn Leu Glu Asp Pro 
105 



Val Asn Thr Asn Met Gly Leu 
120 



Ala Ser 
110 



Lys Phe 
125 



Arg Asp 
Arg Gin 



Leu Leu Trp Phe His 

130 



He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
135 140 



He Glu Tyr Leu Val 
145 



Ser Phe Gly Val Trp He Arg 
150 155 



Pro Ala 
160 



Tyr Arg Pro Pro Asn 
165 



Ala Pro He Leu Ser Thr Leu 
170 



Thr Thr 
175 



Val Val Arg Arg Arg 

180 



Gly Arg Ser Pro Arg Arg Arg 
185 



Thr P: 

190 



ro Ser Pro 



Arg Arg Arg Arg Ser 
195 



Gin Ser Pro Arg Arg Arg Arg 
200 



Ser Gin 
205 



Glu Ser Gin Cys 
210 



<210> 109 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 109 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Thr Cys Pro Thr 



Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 



50 



55 



60 



Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 



-37- 



65 

His Thr Ala Leu 



Leu Ala Thr Trp 
100 

Leu Val Val Ser 
115 

Leu Leu Trp Phe 
130 

lie Glu Tyr Leu 
145 



Tyr Arg Pro Pro 



Val Val Arg Arg 
180 



Arg Arg Arg Arg 
195 

Glu Ser Gin Cys 
210 



70 



Arg Gin Ala lie 
85 

Val Gly Val Asn 



Tyr Val Asn Thr 
120 

His lie Ser Cys 
135 

Val Ala Phe Gly 
150 

Asn Ala Pro lie 
165 

Arg Gly Arg Ser 



Ser Gin Ser Pro 
200 



75 

Leu Cys Trp Gly 
90 



Leu Glu Asp Pro 
105 

Asn Met Gly Leu 



Leu Thr Phe Gly 
140 

Val Trp lie Arg 
155 

Leu Ser Thr Leu 
170 

Pro Arg Arg Arg 
185 

Arg Arg Arg Arg 



80 

Glu Leu Met Thr 
95 

Ala Ser Arg Asp 
110 



Lys Phe Arg Gin 
125 

Arg Glu Thr Val 



Thr Pro Pro Ala 
160 

Pro Glu Thr Thr 
175 

Thr Pro Ser Pro 
190 

Ser Gin Ser Arg 
205 



<210> 110 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 110 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Phe Glu Cys Ser Glu His Cys Ser Pro His 



His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro lie Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 



Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 



-38- 



Ile Glu Tyr Leu Val Ser Phe Gly Val Trp lie Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 111 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<220> 

<221> UNSURE 

<222> (28) . . (28) 

<223> May be any amino acid. 



<400> 111 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Xaa Asp Met Asp lie 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 



His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Asp Leu lie Thr 
85 90 95 

Leu Ser Thr Trp Val Gly Gly Asn Leu Glu Asp Pro Thr Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

lie Glu Tyr Leu Val Ser Phe Gly Val Trp lie Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 



Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 



-39- 



Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Thr Gin Ser Arg 
195 200 205 



Glu Ser Gin Cys 
210 



<210> 112 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 112 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Asn Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

lie Glu Tyr Leu Val Ser Phe Gly Val Trp lie Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 113 
<211> 212 
<212> PRT 
<213> Hepati 



<400> 113 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 



-40- 



15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 



Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His lie Cys Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

lie Glu Tyr Leu Val Ser Phe Gly Val Trp lie Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser Thr Leu Pro Glu Thr Thr 

165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 



Glu Ser Gin Cys 
210 



<210> 114 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 114 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 



Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 



-41- 



Ala Leu Arg Gin Ala 
85 



lie Leu Cys 
90 



Trp Gly Glu Leu Met Thr 
95 



Thr Trp Val Gly Val 
100 



Asn Leu Glu 
105 



Asp Pro Ala Ser 
110 



Val Ser Tyr Val Asn 
115 



Gly Leu Lys Phe 
125 



Leu Leu 
130 



He Glu 
145 



Trp Phe His He Ser 
135 



Tyr Leu Val Ser Phe 
150 



Cys Leu Thr 

Gly Val Trp 



Phe Gly Arg Glu 
140 



He Arg Thr Pro 
155 



Arg Asp 
Arg Gin 
Thr Val 



Pro Ala 
160 



Pro Pro Asn Ala Pro 
165 



He Leu Ser 
170 



Thr Leu Pro Glu 



Thr Thr 
175 



Arg Arg Arg Gly Arg 
180 



Ser Pro Arg 
185 



Arg Arg Thr Pro 
190 



Arg Arg Ser Gin Ser 
195 



Pro Arg Arg 
200 



Arg Arg Ser Gin 
205 



Glu Pro 
210 



<210> 115 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 115 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Ser Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

lie Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 



Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 



Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 



Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 



Glu Ser Gin Cys 
210 



<210> 116 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 116 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

lie Glu Tyr Leu Val Ser Phe Gly Val Trp lie Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro lie Leu Leu Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 117 



<211> 212 
<212> PRT 

<213> Hepatitis B virus 



<400> 117 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Lys Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 

145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 118 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 118 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 



Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 



Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ala 
50 55 60 



Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 



His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Glu Leu Met Thr 

85 90 95 

Leu Ala Thr Trp Val Gly Thr Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

Leu Glu Tyr Leu Val Ser Phe Gly Val Trp lie Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 



Glu Ser Gin Cys 



<210> 119 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 119 

Met Asp lie Asp Pro Tyr Lys Glu Phe Gly Ala Ser Met Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Tyr Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Thr Pro His His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Glu 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Gly Asn Leu Gin Asp Pro Thr 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His Val Ser Cys Leu Thr Phe Gly Arg 
100 105 110 



Glu Thr Val Val Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 



Pro Gin Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 



Glu Thr Cys Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 120 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 120 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg His Val Phe Leu Cys Trp Gly Asp 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro Thr 



Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Thr Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 121 
<211> 212 
<212> PRT 
<213> Hepatitis 



<400> 121 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 



Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp Leu Thr Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 122 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 122 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 



-47- 



His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu He Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 



Glu Ser Gin Cys 
210 



<210> 123 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 123 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Val 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Val Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Thr Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 



Pro Ser Pro Ala Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 



Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 124 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 124 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp lie 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Asp Leu Met Asn 
85 90 95 

Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro Val Ser Arg Asp 
100 105 110 

Leu Val Val Gly Tyr Val Asn Thr Thr Val Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

lie Glu Tyr Leu Val Ser Phe Gly Val Trp lie Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 125 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 



<400> 125 

Met Asp lie Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 



Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val He Glu Tyr Leu Val Ser Phe Gly Val Trp lie Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Thr Val Val Arg Arg Arg Gly Arg Thr Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 126 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 126 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 



Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Ala Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 



Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 



-50- 



Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

lie Leu Trp Phe His lie Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 127 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 127 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Thr Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Val Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 



-51- 



Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 128 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 128 

Met Gin Leu Phe His Leu Cys Leu lie lie Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 



Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Arg He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Thr Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 129 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 129 

Met Gin Leu Phe His Leu Cys Leu Val He Ser Cys Ser Cys Pro Thr 



-52- 



Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ala 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65 70 75 80 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Asn Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 110 

Leu Val Val Asn Tyr Val Asn Thr Asn Met Gly Leu Lys He Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

Leu Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 130 
<211> 212 
<212> PRT 

<213> Hepatitis B virus 
<400> 130 

Met Gin Leu Phe His Leu Cys Leu He He Ser Cys Ser Cys Pro Thr 
15 10 15 

Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp He 
20 25 30 

Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
35 40 45 

Pro Ser Ala Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
50 55 60 

Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 



-53- 

His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp Leu Met Thr 
85 90 95 

Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
100 105 HO 

Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gin 
115 120 125 

Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
130 135 140 

He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala 
145 150 155 160 

Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr 
165 170 175 

Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
180 185 190 

Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
195 200 205 

Glu Ser Gin Cys 
210 



<210> 131 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 131 

Met Asp He Asp Pro 
1 5 

Ser Phe Leu Pro Ser 
20 

Thr Ala Ala Ala Leu 
35 

Ser Pro His His Thr 
50 

Leu Met Thr Leu Ala 
65 

Ser Arg Asp Leu Val 
85 

He Arg Gin Leu Leu 
100 

Glu Thr Val Leu Glu 
115 

Pro Pro Ala Tyr Arg 

130 

Glu Thr Thr Val Val 
145 



Tyr Lys Glu Phe Gly 
10 



Asp Phe Phe Pro Ser 
25 

Tyr Arg Glu Ala Leu 
40 

Ala Leu Arg Gin Ala 
55 

Thr Trp Val Gly Asn 



Val Asn Tyr Val Asn 
90 



Trp Phe His He Ser 
105 

Tyr Leu Val Ser Phe 
120 

Pro Pro Asn Ala Pro 
135 

Arg Arg Arg Gly Arg 
150 



Ala Thr Val Glu Leu Leu 
15 



Val Arg Asp Leu Leu Asp 
30 



Glu Ser Pro Glu His Cys 
45 



He Leu Cys Trp Gly Glu 
60 



Asn Leu Glu Asp Pro Ala 
75 80 



Thr Asn Met Gly Leu Lys 
95 



Cys Leu Thr Phe Gly Arg 
110 



Gly Val Trp He Arg Thr 
125 



He Leu Ser Thr Leu Pro 
140 



Ser Pro Arg Arg Arg Thr 
155 160 



-54- 



Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Glu Ser Gin Cys 
180 



<210> 132 
<211> 183 
<212> PRT 

<213> Hepatitis B virus 
<400> 132 

Met Asp lie Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Gly Asn Leu Glu Asp Pro He 
65 70 75 80 

Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys 
85 90 95 

Phe Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

Glu Thr Val He Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 

Glu Thr Cys Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr 
145 150 155 160 

Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser 
165 170 175 

Gin Ser Arg Gly Ser Gin Cys 
180 

<210> 133 
<211> 3221 
<212> DNA 

<213> Hepatitis B virus 

<220> 
<221> CDS 

<222> (1901) . . (2458) 
<400> 133 

ttccactgcc ttccaccaag ctctgcagga ccccagagtc aggggtctgt attttcctgc 
tggtggctcc agttcaggaa cagtaaaccc tgctccgaat attgcctctc acatctcgtc 



-55- 

aatctccgcg aggactgggg accctgtgac gaacatggag aacatcacat caggattcct 180 

aggacccctg ctcgtgttac aggcggggtt tttattgttg acaagaatcc tcacaatacc 240 

gcagagtcta gactcgtggt ggacttctct caattttata gggggatcac ccgtgtgtct 300 

tggccaaaat tcgcagtccc caacctccaa tcactcacca acctcctgtc ctccaatttg 360 

tcctggttat cgctggatgt gtctgcggcg ttttatcata ttcctcttca tcctgctgct 420 

atgcctcatc ttcttattgg ttcttctgga ttatcaaggt atgttgcccg tttgtcctct 480 

aattccagga tcaacaacaa ccagtacggg accatgcaaa acctgcacga ctcctgctca 540 

aggcaactct atgtttccct catgttgctg tacaaaacct acggttggaa attgcacctg 600 

tattcccatc ccatcgtcct gggctttcgc aaaataccta tgggagtggg cctcagtccg 660 

tttctcttgg ctcagtttac tagtgccatt tgttcagtgg ttcgtagggc tttcccccac 720 

tgtttggctt tcagctatat ggatgatgtg gtattggggg ccaagtctgt acagcatcgt 780 

gagtcccttt ataccgctgt taccaatttt cttttgtctc tgggtataca tttaaaccct 840 

aacaaaacaa aaagatgggg ttattcccta aacttcatgg gttacataat tggaagttgg 900 

ggaacattgc cacaggatca tattgtacaa aagatcaaac actgttttag aaaacttcct 960 

gttaacaggc ctattgattg gaaagtatgt caaagaattg tgggtctttt gggctttgct 1020 

gctccattta cacaatgtgg atatcctgcc ttaatgcctt tgtatgcatg tatacaggct 1080 

aaacaggctt tcactttctc gccaacttac aaggcctttc taagtaaaca gtacatgaac 1140 

ctttaccccg ttgctcggca acggcctggt ctgtgccaag tgtttgctga cgcaaccccc 1200 

actggttggg gcttggccat aggccatcag cgcatgagtg gaacctttgt ggctcctctg 1260 

ccgatccata ctgcggaact cctagccgct tgtattgctc gcagccggtc tggagcaaag 1320 

ctcatcggaa ctgacaattc tgtcgtcctc tcgcggaaat atacatcgtt tccatggctg 1380 

ctaggctgta ctgccaactg gatccttcgc gggacgtcct ttgtttacgt cccgtcggcg 1440 

ctgaatcccg cggacgaccc ctctcggggc cgcttgggac tctatcgtcc ccttctccgt 1500 

ctgccgttcc agccgaccac ggggcgcacc tctctttacg cggtctcccc gtctgtgcct 1560 

tctcatctgc cggtccgtgt gcacttcgct tcacctctgc acgttgcatg gagaccaccg 1620 

tgaacgccca tcagatcctg cccaaggtct tacataagag gactcttgga ctcccagcaa 1680 

tgtcaacgac cgaccttgag gcctacttca aagactgtgt gtttaaggac tgggaggagc 1740 

tgggggagga gattaggtta aaggtctttg tattaggagg ctgtaggcat aaattggtct 1800 

gcgcaccagc accatgcaac tttttcacct ctgcctaatc atctcttgta catgtcccac 1860 

tgttcaagcc tccaagctgt gccttgggtg gctttggggc atg gac att gac cct 1915 

Met Asp lie Asp Pro 
1 5 

tat aaa gaa ttt gga get act gtg gag tta etc teg ttt ttg cct tct 1963 



-56- 



Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu Pro Ser 
10 15 20 

gac ttc ttt cct tec gtc aga gat etc eta gac ace gec tea get ctg 2011 
Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser Ala Leu 
25 30 35 

tat cga gaa gee tta gag tct cct gag cat tgc tea cct cac cat act 2059 
Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His His Thr 
40 45 50 

gca etc agg caa gee att etc tgc tgg ggg gaa ttg atg act eta get 2107 
Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu Leu Met Thr Leu Ala 
55 60 65 

acc tgg gtg ggt aat aat ttg gaa gat cca gca tec agg gat eta gta 2155 
Thr Trp Val Gly Asn Asn Leu Glu Asp Pro Ala Ser Arg Asp Leu Val 
70 75 80 85 

gtc aat tat gtt aat act aac atg ggt tta aag ate agg caa eta ttg 2203 
Val Asn Tyr Val Asn Thr Asn Met Gly Leu Lys He Arg Gin Leu Leu 
90 95 100 

tgg ttt cat ata tct tgc ctt act ttt gga aga gag act gta ctt gaa 2251 
Trp Phe His He Ser Cys Leu Thr Phe Gly Arg Glu Thr Val Leu Glu 
105 HO 115 

tat ttg gtc tct ttc gga gtg tgg att cgc act cct cca gec tat aga 2299 
Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Pro Ala Tyr Arg 
120 125 130 

cca cca aat gee cct ate tta tea aca ctt ccg gaa act act gtt gtt 2347 
Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu Thr Thr Val Val 
135 140 145 

aga cga egg gac cga ggc agg tec cct aga aga aga act ccc teg cct 2395 
Arg Arg Arg Asp Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
150 155 160 165 

cgc aga cgc aga tct caa teg ccg cgt cgc aga aga tct caa tct egg 2443 
Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg Arg Ser Gin Ser Arg 
170 175 180 

gaa tct caa tgt tag tattccttgg actcataagg tgggaaactt tactgggctt 2498 
Glu Ser Gin Cys 
185 

tattcctcta cagtacctat ctttaatcct gaatggcaaa ctccttcctt tcctaagatt 2558 

catttacaag aggacattat tgataggtgt caacaatttg tgggccctct cactgtaaat 2618 

gaaaagagaa gattgaaatt aattatgect gctagattct atcctaccca cactaaatat 2678 

ttgeccttag acaaaggaat taaaccttat tatccagatc aggtagttaa tcattacttc 2738 

caaaccagac attatttaca tactctttgg aaggctggta ttctatataa gagggaaacc 27 98 

acaegtageg catcattttg cgggtcacca tattcttggg aacaagagct acagcatggg 2858 

aggttggtca ttaaaacctc gcaaaggcat ggggacgaat ctttctgttc ccaaccctct 2 918 

gggattcttt cccgatcatc agttggaccc tgcattcgga gccaactcaa acaatccaga 2978 



ttgggacttc aaccccatca aggaccactg 
attcgggcca gggctcaccc ctccacacgg 
gggcatattg accacagtgt caacaattcc 
aaggcagcct actcccatct ctccacctct aa< 
gaa 



<210> 134 
<211> 185 
<212> PRT 

<213> Hepatitis B virus 
<400> 134 



Met 


Asp 


He 


Asp 


Pro 


Tyr 


Lys 


Glu 


Phe 


1 








5 










Ser 


Phe 


Leu 


Pro 


Ser 


Asp 


Phe 


Phe 


Pro 








20 










25 


Thr 


Ala 


Ser 


Ala 


Leu 


Tyr 


Arg 


Glu 


Ala 






35 










40 




Ser 


Pro 


His 


His 


Thr 


Ala 


Leu 


Arg 


Gin 




50 










55 






Leu 


Met 


Thr 


Leu 


Ala 


Thr 


Trp 


Val 


Gly 


65 










70 








Ser 


Arg 


Asp 


Leu 


Val 
85 


Val 


Asn 


Tyr 


Val 


He 


Arg 


Gin 


Leu 


Leu 


Trp 


Phe 


His 


He 








100 










105 


Glu 


Thr 


Val 


Leu 


Glu 


Tyr 


Leu 


Val 


Ser 






115 










120 




Pro 


Pro 


Ala 


Tyr 


Arg 


Pro 


Pro 


Asn 


Ala 




130 










135 






Glu 


Thr 


Thr 


Val 


Val 


Arg 


Arg 


Arg 


Asp 


145 










150 








Arg 


Thr 


Pro 


Ser 


Pro 


Arg 


Arg 


Arg 


Arg 










165 










Arg 


Ser 


Gin 


Ser 


Arg 


Glu 


Ser 


Gin 


Cys 








180 










185 



-57- 

gccagcagcc aaccaggtag gagtgggagc 3038 
cggtattttg gggtggagcc ctcaggctca 3098 
tcctcctgcc tccaccaatc ggcagtcagg 3158 
facagt catcctcagg ccatgcagtg 3218 
3221 



Gly 


Ala 


Thr 


Val 


Glu 


Leu 


Leu 


10 










15 




Ser 


Val 


Arg 


Asp 


Leu 


Leu 


Asp 










30 






Leu 


Glu 


Ser 


Pro 


Glu 


His 


Cys 








45 








Ala 


He 


Leu 


Cys 


Trp 


Gly 


Glu 






60 










Asn 


Asn 


Leu 


Glu 


Asp 


Pro 


Ala 




75 










80 


Asn 


Thr 


Asn 


Met 


Gly 


Leu 


Lys 


90 










95 




Ser 


Cys 


Leu 


Thr 


Phe 


Gly 


Arg 










110 






Phe 


Gly 


Val 


Trp 


He 


Arg 


Thr 








125 








Pro 


He 


Leu 


Ser 


Thr 


Leu 


Pro 






140 










Arg 


Gly 


Arg 


Ser 


Pro 


Arg 


Arg 




155 










160 


Ser 


Gin 


Ser 


Pro 


Arg 


Arg 


Arg 


170 










175 





<210> 135 
<211> 188 
<212> PRT 

<213> Woodchuck hepatitis B virus 
<400> 135 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ser Ser Tyr Gin Leu Leu 
15 10 15 

Asn Phe Leu Pro Leu Asp Phe Phe Pro Asp Leu Asn Ala Leu Val Asp 
20 25 30 

Thr Ala Thr Ala Leu Tyr Glu Glu Glu Leu Thr Gly Arg Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala He Arg Gin Ala Leu Val Cys Trp Asp Glu 
50 55 60 

Leu Thr Lys Leu He Ala Trp Met Ser Ser Asn He Thr Ser Glu Gin 



65 



Val Arg Thr He 



Val Arg Gin Ser 
100 

His Thr Val Gin 
115 

Pro Ala Pro Tyr 
130 

Glu His Thr Val 
145 



Pro Arg Arg Arg 



Arg Arg Arg Arg 
180 



70 

He Val Asn His 
85 

Leu Trp Phe His 



Glu Phe Leu Val 
120 



Arg Pro Pro Asn 
135 

He Arg Arg Arg 
150 

Thr Pro Ser Pro 
165 

Ser Gin Ser Pro 



-58- 



75 

Val Asn Asp Thr 
90 

Leu Ser Cys Leu 
105 

Ser Phe Gly Val 



Ala Pro He Leu 
140 

Gly Gly Ala Arg 
155 

Arg Arg Arg Arg 
170 

Ser Thr Asn Cys 
185 



80 

Trp Gly Leu Lys 
95 

Thr Phe Gly Gin 
110 

Trp He Arg Thr 
125 

Ser Thr Leu Pro 



Ala Ser Arg Ser 
160 

Ser Gin Ser Pro 
175 



<210> 136 
<211> 217 
<212> PRT 

<213> Ground squirrel hepatitis virus 
<400> 136 

Met Tyr Leu Phe His Leu Cys Leu Val Phe Ala Cys Val Pro Cys Pro 
15 10 15 

Thr Val Gin Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Asp Met Asp 
20 25 30 

He Asp Pro Tyr Lys Glu Phe Gly Ser Ser Tyr Gin Leu Leu Asn Phe 
35 40 45 

Leu Pro Leu Asp Phe Phe Pro Asp Leu Asn Ala Leu Val Asp Thr Ala 
50 55 60 

Ala Ala Leu Tyr Glu Glu Glu Leu Thr Gly Arg Glu His Cys Ser Pro 
65 70 75 80 

His His Thr Ala He Arg Gin Ala Leu Val Cys Trp Glu Glu Leu Thr 
85 90 95 

Arg Leu He Thr Trp Met Ser Glu Asn Thr Thr Glu Glu Val Arg Arg 
100 105 110 

He He Val Asp His Val Asn Asn Thr Trp Gly Leu Lys Val Arg Gin 
115 120 125 

Thr Leu Trp Phe His Leu Ser Cys Leu Thr Phe Gly Gin His Thr Val 
130 135 140 

Gin Glu Phe Leu Val Ser Phe Gly Val Trp He Arg Thr Pro Ala Pro 
145 150 155 160 



Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro Glu His Thr 
165 170 175 



Val lie Arg Arg Arg Gly Gly Ser Arg Ala Ala Arg Ser Pro Arg Arg 
180 185 190 



Arg Thr Pro Ser Pro Arg Arg Arg Arg Ser Gin Ser Pro Arg Arg Arg 
195 200 205 

Arg Ser Gin Ser Pro Ala Ser Asn Cys 
210 215 



<210> 137 
<211> 262 
<212> PRT 

<213> Snow Goose Hepatitis B Virus 
<400> 137 

Met Asp Val Asn Ala Ser Arg Ala Leu Ala Asn Val Tyr Asp Leu Pro 
15 10 15 

Asp Asp Phe Phe Pro Lys lie Glu Asp Leu Val Arg Asp Ala Lys Asp 
20 25 30 

Ala Leu Glu Pro Tyr Trp Lys Ser Asp Ser lie Lys Lys His Val Leu 
35 40 45 

lie Ala Thr His Phe Val Asp Leu lie Glu Asp Phe Trp Gin Thr Thr 
50 55 60 

Gin Gly Met His Glu lie Ala Glu Ala He Arg Ala Val He Pro Pro 
65 70 75 80 

Thr Thr Ala Pro Val Pro Ser Gly Tyr Leu He Gin His Asp Glu Ala 
85 90 95 

Glu Glu He Pro Leu Gly Asp Leu Phe Lys Glu Gin Glu Glu Arg He 
100 105 110 

Val Ser Phe Gin Pro Asp Tyr Pro He Thr Ala Arg He His Ala His 
115 120 125 

Leu Lys Ala Tyr Ala Lys He Asn Glu Glu Ser Leu Asp Arg Ala Arg 
130 135 140 

Arg Leu Leu Trp Trp His Tyr Asn Cys Leu Leu Trp Gly Glu Ala Thr 
145 150 155 160 

Val Thr Asn Tyr He Ser Arg Leu Arg Thr Trp Leu Ser Thr Pro Glu 
165 170 175 

Lys Tyr Arg Gly Arg Asp Ala Pro Thr He Glu Ala He Thr Arg Pro 
180 185 190 

He Gin Val Ala Gin Gly Gly Arg Lys Thr Ser Thr Ala Thr Arg Lys 
195 200 205 

Pro Arg Gly Leu Glu Pro Arg Arg Arg Lys Val Lys Thr Thr Val Val 
210 215 220 

Tyr Gly Arg Arg Arg Ser Lys Ser Arg Glu Arg Arg Ala Ser Ser Pro 
225 230 235 240 

Gin Arg Ala Gly Ser Pro Leu Pro Arg Ser Ser Ser Ser His His Arg 
245 250 255 



A 



-60- 



Ser Pro Ser Pro Arg Lys 



<210> 138 
<211> 305 
<212> PRT 

<213> Duck hepatitis B virus 
<400> 138 

Met Trp Asp Leu Arg Leu His Pro Ser Pro Phe Gly Ala Ala Cys Gin 
15 10 15 

Gly He Phe Thr Ser Ser Leu Leu Leu Phe Leu Val Thr Val Pro Leu 
20 25 30 

Val Cys Thr He Val Tyr Asp Ser Cys Leu Cys Met Asp He Asn Ala 
35 40 45 

Ser Arg Ala Leu Ala Asn Val Tyr Asp Leu Pro Asp Asp Phe Phe Pro 
50 55 60 

Lys He Asp Asp Leu Val Arg Asp Ala Lys Asp Ala Leu Glu Pro Tyr 
65 70 75 80 

Trp Arg Asn Asp Ser He Lys Lys His Val Leu He Ala Thr His Phe 
85 90 95 

Val Asp Leu He Glu Asp Phe Trp Gin Thr Thr Gin Gly Met His Glu 
100 105 110 

He Ala Glu Ala Leu Arg Ala He He Pro Ala Thr Thr Ala Pro Val 
115 120 125 

Pro Gin Gly Phe Leu Val Gin His Glu Glu Ala Glu Glu He Pro Leu 
130 135 140 

Gly Glu Leu Phe Arg Tyr Gin Glu Glu Arg Leu Thr Asn Phe Gin Pro 
145 150 155 160 

Asp Tyr Pro Val Thr Ala Arg He His Ala His Leu Lys Ala Tyr Ala 
165 170 175 

Lys He Asn Glu Glu Ser Leu Asp Arg Ala Arg Arg Leu Leu Trp Trp 
180 185 190 

His Tyr Asn Cys Leu Leu Trp Gly Glu Pro Asn Val Thr Asn Tyr lie 
195 200 205 

Ser Arg Leu Arg Thr Trp Leu Ser Thr Pro Glu Lys Tyr Arg Gly Lys 
210 215 220 

Asp Ala Pro Thr He Glu Ala He Thr Arg Pro He Gin Val Ala Gin 
225 230 235 240 

Gly Gly Arg Asn Lys Thr Gin Gly Val Arg Lys Ser Arg Gly Leu Glu 
245 250 255 

Pro Arg Arg Arg Arg Val Lys Thr Thr He Val Tyr Gly Arg Arg Arg 
260 265 270 

Ser Lys Ser Arg Glu Arg Arg Ala Pro Thr Pro Gin Arg Ala Gly Ser 
275 280 285 



-61- 



Pro Leu Pro Arg Thr Ser Arg Asp His His Arg Ser Pro Ser Pro Arg 
290 295 300 

Glu 

305 



<210> 139 
<211> 212 
<212> PRT 

<213> Haemophilus influenzae 
<400> 139 

Met Lys Lys Thr Leu Leu Gly Ser Leu lie Leu Leu Ala Phe Ala Gly 
15 10 15 

Asn Val Gin Ala Ala Ala Asn Ala Asp Thr Ser Gly Thr Val Thr Phe 
20 25 30 

Phe Gly Lys Val Val Glu Asn Thr Cys Gin Val Asn Gin Asp Ser Glu 
35 40 45 

Tyr Glu Cys Asn Leu Asn Asp Val Gly Lys Asn His Leu Ser Gin Gin 
50 55 60 

Gly Tyr Thr Ala Met Gin Thr Pro Phe Thr lie Thr Leu Glu Asn Cys 
65 70 75 80 

Asn Val Thr Thr Thr Asn Asn Lys Pro Lys Ala Thr Lys Val Gly Val 
85 90 95 

Tyr Phe Tyr Ser Trp Glu lie Ala Asp Lys Asp Asn Lys Tyr Thr Leu 
100 105 110 

Lys Asn lie Lys Glu Asn Thr Gly Thr Asn Asp Ser Ala Asn Lys Val 
115 120 125 

Asn lie Gin Leu Leu Glu Asp Asn Gly Thr Ala Glu lie Lys Val Val 
130 135 140 

Gly Lys Thr Thr Thr Asp Phe Thr Ser Glu Asn His Asn Gly Ala Gly 
145 150 155 160 

Ala Asp Pro Val Ala Thr Asn Lys His lie Ser Ser Leu Thr Pro Leu 
165 170 175 

Asn Asn Gin Asn Ser lie Asn Leu His Tyr lie Ala Gin Tyr Tyr Ala 
180 185 190 

Thr Gly Val Ala Glu Ala Gly Lys Val Pro Ser Ser Val Asn Ser Gin 
195 200 205 

lie Ala Tyr Glu 
210 



<210> 140 
<211> 139 
<212> PRT 

<213> Pseudomonas stutzeri 



<400> 140 

Met Lys Ala Gin Met Gin Lys Gly Phe Thr Leu lie Glu Leu Met lie 



-62- 



Val Val Ala lie lie Gly lie Leu Ala Ala lie Ala Leu Pro Ala Tyr 
20 25 30 

Gin Asp Tyr Thr Val Arg Ser Asn Ala Ala Ala Ala Leu Ala Glu lie 
35 40 45 

Thr Pro Gly Lys He Gly Phe Glu Gin Ala He Asn Glu Gly Lys Thr 
50 55 60 

Pro Ser Leu Thr Ser Thr Asp Glu Gly Tyr He Gly He Thr Asp Ser 
65 70 75 80 

Thr Ser Tyr Cys Asp Val Asp Leu Asp Thr Ala Ala Asp Gly His He 
85 90 95 

Glu Cys Thr Ala Lys Gly Gly Asn Ala Gly Lys Phe Asp Gly Lys Thr 
100 105 110 

He Thr Leu Asn Arg Thr Ala Asp Gly Glu Trp Ser Cys Ala Ser Thr 
115 120 125 

Leu Asp Ala Lys Tyr Lys Pro Gly Lys Cys Ser 
130 135 



<210> 141 
<211> 59 
<212> PRT 

<213> Caulobacter crescentus 
<400> 141 

Met Thr Lys Phe Val Thr Arg Phe 
1 5 

Ala He Glu Tyr Gly Leu He Val 
20 

Thr Ala Val Thr Thr Leu Gly Thr 

35 40 

Ala Gly Ala Ala Val Ser Thr Ala 
50 55 



Leu Lys Asp Glu Ser Gly Ala Thr 
10 15 

Ala Leu He Ala Val Val He Val 
25 30 

Asn Leu Arg Thr Ala Phe Thr Lys 
45 

Ala Gly Thr 



<210> 142 
<211> 173 
<212> PRT 

<213> Escherichia coli 
<400> 142 

Met Ala Val Val Ser Phe Gly Val Asn Ala Ala Pro Thr He Pro Gin 
15 10 15 

Gly Gin Gly Lys Val Thr Phe Asn Gly Thr Val Val Asp Ala Pro Cys 
20 25 30 

Ser He Ser Gin Lys Ser Ala Asp Gin Ser He Asp Phe Gly Gin Leu 
35 40 45 



Ser Lys Ser Phe Leu Glu Ala Gly Gly Val Ser Lys Pro Met Asp Leu 
50 55 60 



-63- 



Asp lie Glu Leu Val Asn Cys Asp lie Thr Ala Phe Lys Gly Gly Asn 
65 70 75 80 

Gly Ala Gin Lys Gly Thr Val Lys Leu Ala Phe Thr Gly Pro lie Val 
85 90 95 



Asn Gly His Ser Asp Glu Leu Asp Thr Asn Gly Gly Thr Gly Thr Ala 
100 105 110 



lie Val Val Gin Gly Ala Gly Lys Asn Val Val Phe Asp Gly Ser Glu 
115 120 125 

Gly Asp Ala Asn Thr Leu Lys Asp Gly Glu Asn Val Leu His Tyr Thr 
130 135 140 

Ala Val Val Lys Lys Ser Ser Ala Val Gly Ala Ala Val Thr Glu Gly 
145 150 155 160 

Ala Phe Ser Ala Val Ala Asn Phe Asn Leu Thr Tyr Gin 
165 170 



<210> 143 
<211> 173 
<212> PRT 

<213> Escherichia coli 
<400> 143 

Met Ala Val Val Ser Phe Gly Val Asn Ala Ala Pro Thr He Pro Gin 
15 10 15 

Gly Gin Gly Lys Val Thr Phe Asn Gly Thr Val Val Asp Ala Pro Cys 
20 25 30 

Ser He Ser Gin Lys Ser Ala Asp Gin Ser He Asp Phe Gly Gin Leu 



Ser Lys Ser Phe Leu Glu Ala Gly Gly Val Ser Lys Pro Met Asp Leu 
50 55 60 

Asp He Glu Leu Val Asn Cys Asp He Thr Ala Phe Lys Gly Gly Asn 
65 70 75 80 

Gly Ala Gin Lys Gly Thr Val Lys Leu Ala Phe Thr Gly Pro He Val 
85 90 95 

Asn Gly His Ser Asp Glu Leu Asp Thr Asn Gly Gly Thr Gly Thr Ala 
100 105 110 

He Val Val Gin Gly Ala Gly Lys Asn Val Val Phe Asp Gly Ser Glu 
115 120 125 

Gly Asp Ala Asn Thr Leu Lys Asp Gly Glu Asn Val Leu His Tyr Thr 
130 135 140 

Ala Val Val Lys Lys Ser Ser Ala Val Gly Ala Ala Val Thr Glu Gly 
145 150 155 160 

Ala Phe Ser Ala Val Ala Asn Phe Asn Leu Thr Tyr Gin 
165 170 



<210> 144 
<211> 172 



<212> PRT 

<213> Escherichia coli 



<400> 144 

Met Ala Val Val Ser Phe Gly Val Asn Ala Ala Pro Thr Thr Pro Gin 
15 10 15 

Gly Gin Gly Arg Val Thr Phe Asn Gly Thr Val Val Asp Ala Pro Cys 
20 25 30 

Ser He Ser Gin Lys Ser Ala Asp Gin Ser He Asp Phe Gly Gin Leu 
35 40 45 

Ser Lys Ser Phe Leu Ala Asn Asp Gly Gin Ser Lys Pro Met Asn Leu 
50 55 60 

Asp He Glu Leu Val Asn Cys Asp He Thr Ala Phe Lys Asn Gly Asn 
65 70 75 80 

Ala Lys Thr Gly Ser Val Lys Leu Ala Phe Thr Gly Pro Thr Val Ser 
85 90 95 

Gly His Pro Ser Glu Leu Ala Thr Asn Gly Gly Pro Gly Thr Ala He 
100 105 110 

Met He Gin Ala Ala Gly Lys Asn Val Pro Phe Asp Gly Thr Glu Gly 
115 120 125 

Asp Pro Asn Leu Leu Lys Asp Gly Asp Asn Val Leu His Tyr Thr Thr 
130 135 140 

Val Gly Lys Lys Ser Ser Asp Gly Asn Ala Gin He Thr Glu Gly Ala 
145 150 155 160 

Phe Ser Gly Val Ala Thr Phe Asn Leu Ser Tyr Gin 
165 170 



<210> 145 
<211> 853 
<212> DNA 

<213> Escherichia coli 

<220> 

<221> CDS 

<222> (281) . . (829) 

<400> 145 

acgtttctgt ggctcgacgc atcttcctca ttcttctctc caaaaaccac ctcatgcaat 60 

ataaacatct ataaataaag ataacaaata gaatattaag ccaacaaata aactgaaaaa 120 

gtttgtccgc gatgctttac ctctatgagt caaaatggcc ccaatgtttc atcttttggg 180 

ggaaactgtg cagtgttggc agtcaaactc gttgacaaac aaagtgtaca gaacgactgc 240 

ccatgtcgat ttagaaatag ttttttgaaa ggaaagcagc atg aaa att aaa act 295 

Met Lys He Lys Thr 
1 5 



ctg gca ate gtt gtt ctg teg get ctg tec etc agt tct acg acg get 343 
Leu Ala He Val Val Leu Ser Ala Leu Ser Leu Ser Ser Thr Thr Ala 
10 15 20 



ctg gcc get gec acg acg gtt aat ggt ggg acc gtt cac ttt aaa ggg 391 
Leu Ala Ala Ala Thr Thr Val Asn Gly Gly Thr Val His Phe Lys Gly 
25 30 35 



gaa gtt gtt aac gcc get tgc gca gtt gat gca ggc tct gtt gat caa 
Glu Val Val Asn Ala Ala Cys Ala Val Asp Ala Gly Ser Val Asp Gin 



acc gtt cag tta gga cag gtt cgt acc gca teg ctg gca cag gaa gga 
Thr Val Gin Leu Gly Gin Val Arg Thr Ala Ser Leu Ala Gin Glu Gly 
55 60 65 

gca acc agt tct get gtc ggt ttt aac att cag ctg aat gat tgc gat 
Ala Thr Ser Ser Ala Val Gly Phe Asn lie Gin Leu Asn Asp Cys Asp 



acc aat gtt gca tct aaa gcc get gtt gcc ttt tta ggt acg gcg att 
Thr Asn Val Ala Ser Lys Ala Ala Val Ala Phe Leu Gly Thr Ala lie 
90 95 100 

gat gcg ggt cat acc aac gtt ctg get ctg cag agt tea get gcg ggt 
Asp Ala Gly His Thr Asn Val Leu Ala Leu Gin Ser Ser Ala Ala Gly 
105 110 115 

age gca aca aac gtt ggt gtg cag ate ctg gac aga acg ggt get gcg 
Ser Ala Thr Asn Val Gly Val Gin He Leu Asp Arg Thr Gly Ala Ala 
120 125 130 

ctg acg ctg gat ggt gcg aca ttt agt tea gaa aca acc ctg aat aac 
Leu Thr Leu Asp Gly Ala Thr Phe Ser Ser Glu Thr Thr Leu Asn Asn 
135 140 145 

gga acc aat acc att ccg ttc cag gcg cgt tat ttt gca acc ggg gcc 
Gly Thr Asn Thr He Pro Phe Gin Ala Arg Tyr Phe Ala Thr Gly Ala 
150 155 160 165 

gca acc ccg ggt get get aat gcg gat gcg acc ttc aag gtt cag tat 
Ala Thr Pro Gly Ala Ala Asn Ala Asp Ala Thr Phe Lys Val Gin Tyr 
170 175 180 

caa taa cctacctagg ttcagggacg ttca 



<210> 146 
<211> 182 
<212> PRT 

<213> Escherichia coli 



<400> 146 



Met 


Lys 


He 


Lys 


Thr 


Leu 


Ala 


He 


Val 


Val 


Leu 


Ser 


Ala 


Leu 


Ser 


Leu 


1 








5 










10 










15 




Ser 


Ser 


Thr 


Thr 


Ala 


Leu 


Ala 


Ala 


Ala 


Thr 


Thr 


Val 


Asn 


Gly 


Gly 


Thr 








20 










25 










30 






Val 


His 


Phe 


Lys 


Gly 


Glu 


Val 


Val 


Asn 


Ala 


Ala 


Cys 


Ala 


Val 


Asp 


Ala 






35 










40 










45 








Gly 


Ser 


Val 


Asp 


Gin 


Thr 


Val 


Gin 


Leu 


Gly 


Gin 


Val 


Arg 


Thr 


Ala 


Ser 




50 










55 










60 










Leu 


Ala 


Gin 


Glu 


Gly 


Ala 


Thr 


Ser 


Ser 


Ala 


Val 


Gly 


Phe 


Asn 


He 


Gin 


65 










70 










75 










80 


Leu 


Asn 


Asp 


Cys 


Asp 


Thr 


Asn 


Val 


Ala 


Ser 


Lys 


Ala 


Ala 


Val 


Ala 


Phe 










85 










90 










95 




Leu 


Gly 


Thr 


Ala 


He 


Asp 


Ala 


Gly 


His 


Thr 


Asn 


Val 


Leu 


Ala 


Leu 


Gin 



-66- 

100 105 110 

Ser Ser Ala Ala Gly Ser Ala Thr Asn Val Gly Val Gin lie Leu Asp 

115 120 125 

Arg Thr Gly Ala Ala Leu Thr Leu Asp Gly Ala Thr Phe Ser Ser Glu 

130 135 140 

Thr Thr Leu Asn Asn Gly Thr Asn Thr lie Pro Phe Gin Ala Arg Tyr 
145 150 155 160 

Phe Ala Thr Gly Ala Ala Thr Pro Gly Ala Ala Asn Ala Asp Ala Thr 

165 170 175 

Phe Lys Val Gin Tyr Gin 
180 



<210> 147 
<211> 11 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> FLAG peptide 
<400> 147 

Cys Gly Gly Asp Tyr Lys Asp Asp Asp Asp Lys 
15 10 



<210> 148 
<211> 31 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> primer 
<400> 148 

ccggaattca tggacattga cccttataaa g 31 



<210> 149 
<211> 37 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> primer 
<400> 149 

gtgcagtatg gtgaggtgag gaatgctcag gagactc 



<210> 150 
<211> 37 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> primer 



<400> 150 

gsgtctcctg agcattcctc acctcaccat actgcac 



37 



-67- 



<210> 151 
<211> 33 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> primer 
<400> 151 

cttccaaaag tgagggaaga aatgtgaaac cac 



<210> 152 
<211> 47 
<212> DNA 

<213> Artificial Sequence 



<220> 

<223> primer 
<400> 152 

cgcgtcccaa gcttctaaac aacagtagtc tccggaagcg ttgatag 47 



<210> 153 
<211> 33 
<212> DNA 

<213> Artificial Seque 



<220> 

<223> primer 
<400> 153 

gtggtttcac atttcttccc tcacttttgg aag 33 



<210> 154 
<211> 281 
<212> PRT 

<213> Saccharomyces cerevisiae 
<400> 154 

Met Ser Glu Tyr Gin Pro Ser Leu Phe Ala Leu Asn Pro Met Gly Phe 
15 10 15 

Ser Pro Leu Asp Gly Ser Lys Ser Thr Asn Glu Asn Val Ser Ala Ser 
20 25 30 

Thr Ser Thr Ala Lys Pro Met Val Gly Gin Leu lie Phe Asp Lys Phe 
35 40 45 

lie Lys Thr Glu Glu Asp Pro lie lie Lys Gin Asp Thr Pro Ser Asn 
50 55 60 

Leu Asp Phe Asp Phe Ala Leu Pro Gin Thr Ala Thr Ala Pro Asp Ala 
65 70 75 80 

Lys Thr Val Leu Pro lie Pro Glu Leu Asp Asp Ala Val Val Glu Ser 
85 90 95 



Phe Phe Ser Ser Ser Thr Asp Ser Thr Pro Met Phe Glu Tyr Glu Asn 
100 105 110 



-68- 

Leu Glu Asp Asn Ser Lys Glu Trp Thr Ser Leu Phe Asp Asn Asp He 
115 120 125 

Pro Val Thr Thr Asp Asp Val Ser Leu Ala Asp Lys Ala He Glu Ser 
130 135 140 

Thr Glu Glu Val Ser Leu Val Pro Ser Asn Leu Glu Val Ser Thr Thr 
145 150 155 160 

Ser Phe Leu Pro Thr Pro Val Leu Glu Asp Ala Lys Leu Thr Gin Thr 
165 170 175 

Arg Lys Val Lys Lys Pro Asn Ser Val Val Lys Lys Ser His His Val 
180 185 190 

Gly Lys Asp Asp Glu Ser Arg Leu Asp His Leu Gly Val Val Ala Tyr 
195 200 205 

Asn Arg Lys Gin Arg Ser He Pro Leu Ser Pro He Val Pro Glu Ser 
210 215 220 

Ser Asp Pro Ala Ala Leu Lys Arg Ala Arg Asn Thr Glu Ala Ala Arg 
225 230 235 240 

Arg Ser Arg Ala Arg Lys Leu Gin Arg Met Lys Gin Leu Glu Asp Lys 
245 250 255 

Val Glu Glu Leu Leu Ser Lys Asn Tyr His Leu Glu Asn Glu Val Ala 
260 265 270 

Arg Leu Lys Lys Leu Val Gly Glu Arg 
275 280 



<210> 155 
<211> 181 
<212> PRT 
<213> Esche: 



richia coli 



<400> 155 

Met Lys He Lys Thr Leu Ala He 
1 5 



Ser Ser Thr Ala Ala Leu Ala Ala 
20 



Val His Phe Lys Gly Glu Val Val 
35 40 



Gly Ser Val Asp Gin Thr Val Gin 
50 55 



Leu Ala Gin Glu Gly Ala Thr Ser 
65 70 

Leu Asn Asp Cys Asp Thr Asn Val 
85 



Leu Gly Thr Ala He Asp Ala Gly 

100 



Ser Ser Ala Ala Gly Ser Ala Thr 
115 120 



Val Val Leu Ser Ala Leu Ser Leu 
10 15 

Ala Thr Thr Val Asn Gly Gly Thr 
25 30 

Asn Ala Ala Cys Ala Val Asp Ala 
45 



Leu Gly Gin Val Arg Thr Ala Ser 
60 

Ser Ala Val Gly Phe Asn He Gin 

75 80 

Ala Ser Lys Ala Ala Val Ala Phe 
90 95 

His Thr Asn Val Leu Ala Leu Gin 
105 110 

Asn Val Gly Val Gin He Leu Asp 
125 



Arg Thr Gly Ala Ala Leu Thr Leu Asp Gly Ala Thr Phe Ser Ser Glu 

130 135 140 

Thr Thr Leu Asn Asn Gly Thr Asn Thr lie Pro Phe Gin Ala Arg Tyr 

145 150 155 160 

Phe Ala Gly Ala Ala Thr Pro Gly Ala Ala Asn Ala Asp Ala Thr Phe 

165 170 175 



Lys Val Gin Tyr Gin 
180 



<210> 156 
<211> 447 
<212> DNA 
<213> Hepatitis B 

<220> 

<221> CDS 

<222> (1) . . (447) 

<400> 156 

atg gac att gac cct tat aaa gaa ttt gga get act gtg gag tta etc 

Met Asp lie Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

teg ttt ttg cct tct gac ttc ttt cct tec gta cga gat ctt eta gat 
Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

acc gec gca get ctg tat egg gat gec tta gag tct cct gag cat tgt 
Thr Ala Ala Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys 



tea cct cac cat act gca etc agg caa gca att ctt tgc tgg gga gac 
Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Asp 



tta atg act eta get acc tgg gtg ggt act aat tta gaa gat cca gca 
Leu Met Thr Leu Ala Thr Trp Val Gly Thr Asn Leu Glu Asp Pro Ala 



tct agg gac eta gta gtc agt tat gtc aac act aat gtg ggc eta aag 
Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Val Gly Leu Lys 
85 90 95 

ttc aga caa tta ttg tgg ttt cac att tct tgt etc act ttt gga aga 
Phe Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr Phe Gly Arg 
100 105 110 

gaa acg gtt eta gag tat ttg gtc tct ttt gga gtg tgg att cgc act 
Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp He Arg Thr 
115 120 125 

cct cca gec tat aga cca cca aat gec cct ate eta tea acg ctt ccg 
Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser Thr Leu Pro 
130 135 140 



gag act act gtt gtt 
Glu Thr Thr Val Val 
145 



-70- 



<210> 157 
<211> 149 
<212> PRT 
<213> Hepatitis 

<400> 157 
Met Asp lie Asp 
1 

Ser Phe Leu Pro 
20 

Thr Ala Ala Ala 
35 

Ser Pro His His 
50 

Leu Met Thr Leu 
65 

Ser Arg Asp Leu 



Phe Arg Gin Leu 
100 

Glu Thr Val Leu 
115 

Pro Pro Ala Tyr 
130 

Glu Thr Thr Val 
145 



B 



Pro Tyr Lys Glu 
5 

Ser Asp Phe Phe 



Leu Tyr Arg Asp 
40 

Thr Ala Leu Arg 
55 

Ala Thr Trp Val 
70 

Val Val Ser Tyr 
85 

Leu Trp Phe His 



Glu Tyr Leu Val 
120 

Arg Pro Pro Asn 
135 

Val 



Phe Gly Ala Thr 
10 

Pro Ser Val Arg 
25 

Ala Leu Glu Ser 



Gin Ala He Leu 
60 



Gly Thr Asn Leu 
75 

Val Asn Thr Asn 
90 



He Ser Cys Leu 
105 



Ser Phe Gly Val 



Ala Pro He Leu 
140 



Val Glu Leu Leu 
15 

Asp Leu Leu Asp 
30 

Pro Glu His Cys 
45 

Cys Trp Gly Asp 



Glu Asp Pro Ala 
80 

Val Gly Leu Lys 
95 

Thr Phe Gly Arg 

110 

Trp He Arg Thr 
125 

Ser Thr Leu Pro 



<210> 158 
<211> 152 
<212> PRT 
<213> Hepatitis B 

<400> 158 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ala Ala Leu Tyr Arg Asp Ala Leu Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala lie Leu Cys Trp Gly Asp 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Thr Asn Leu Glu Asp Gly Gly 
65 70 75 80 

Lys Gly Gly Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Val 
85 90 95 

Gly Leu Lys Phe Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr 



-71- 



100 105 110 

Phe Gly Arg Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp 
115 120 125 

lie Arg Thr Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro lie Leu Ser 
130 135 140 

Thr Leu Pro Glu Thr Thr Val Val 
145 150 



<210> 159 

<211> 56 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Oligonucleotide 

<400> 159 

tagatgatta cgccaagctt ataatagaaa tagttttttg aaaggaaagc agcatg 



<210> 160 

<211> 45 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Oligonucleotide 

<400> 160 

gtcaaaggcc ttgtcgacgt tattccatta cgcccgtcat tttgg 



<210> 161 

<211> 4623 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> pFIMAIC 



<400> 161 

agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt 60 

tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 120 

ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 180 

taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 240 

tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 300 

gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 360 



-72- 



atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 420 

ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 480 

cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 540 

ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 600 

aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 660 

ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 720 

gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 780 

ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 840 

gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 900 

ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 960 

tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 1020 

cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 1080 

tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 1140 

atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 1200 

tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 1260 

tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 1320 

ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 1380 

cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 144 0 

ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 1500 

gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 1560 

tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 1620 

gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 1680 

ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 17 40 

tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 1800 

ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 18 60 

tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 1920 

attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 1980 

tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 2040 

ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 2100 

aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 2160 

ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat 2220 

gaccatgatt acgccaagct tataatagaa atagtttttt gaaaggaaag cagcatgaaa 2280 



-73- 



attaaaactc tggcaatcgt tgttctgtcg gctctgtccc tcagttctac agcggctctg 2340 

gccgctgcca cgacggttaa tggtgggacc gttcacttta aaggggaagt tgttaacgcc 2400 

gcttgcgcag ttgatgcagg ctctgttgat caaaccgttc agttaggaca ggttcgtacc 2460 

gcatcgctgg cacaggaagg agcaaccagt tctgctgtcg gttttaacat tcagctgaat 2520 

gattgcgata ccaatgttgc atctaaagcc gctgttgcct ttttaggtac ggcgattgat 2580 

gcgggtcata ccaacgttct ggctctgcag agttcagctg cgggtagcgc aacaaacgtt 2640 

ggtgtgcaga tcctggacag aacgggtgct gcgctgacgc tggatggtgc gacatttagt 2700 

tcagaaacaa ccctgaataa cggaaccaat accattccgt tccaggcgcg ttattttgca 27 60 

accggggccg caaccccggg tgctgctaat gcggatgcga ccttcaaggt tcagtatcaa 2820 

taacctaccc aggttcaggg acgtcattac gggcagggat gcccaccctt gtgcgataaa 2880 

aataacgatg aaaaggaaga gattatttct attagcgtcg ttgctgccaa tgtttgctct 2940 

ggccggaaat aaatggaata ccacgttgcc cggcggaaat atgcaatttc agggcgtcat 3000 

tattgcggaa acttgccgga ttgaagccgg tgataaacaa atgacggtca atatggggca 3060 

aatcagcagt aaccg.gtttc atgcggttgg ggaagatagc gcaccggtgc cttttgttat 3120 

tcatttacgg gaatgtagca cggtggtgag tgaacgtgta ggtgtggcgt ttcacggtgt 3180 

cgcggatggt aaaaatccgg atgtgctttc cgtgggagag gggccaggga tagccaccaa 3240 

tattggcgta gcgttgtttg atgatgaagg aaacctcgta ccgattaatc gtcctccagc 3300 

aaactggaaa cggctttatt caggctctac ttcgctacat ttcatcgcca aatatcgtgc 3360 

taccgggcgt cgggttactg gcggcatcgc caatgcccag gcctggttct ctttaaccta 3420 

tcagtaattg ttcagcagat aatgtgataa caggaacagg acagtgagta ataaaaacgt 3480 

caatgtaagg aaatcgcagg aaataacatt ctgcttgctg gcaggtatcc tgatgttcat 3540 

ggcaatgatg gttgccggac gcgctgaagc gggagtggcc ttaggtgcga ctcgcgtaat 3600 

ttatccggca gggcaaaaac aagagcaact tgccgtgaca aataatgatg aaaatagtac 3660 

ctatttaatt caatcatggg tggaaaatgc cgatggtgta aaggatggtc gttttatcgt 3720 

gacgcctcct ctgtttgcga tgaagggaaa aaaagagaat accttacgta ttcttgatgc 3780 

aacaaataac caattgccac aggaccggga aagtttattc tggatgaacg ttaaagcgat 3840 

tccgtcaatg gataaatcaa aattgactga gaatacgcta cagctcgcaa ttatcagccg 3900 

cattaaactg tactatcgcc cggctaaatt agcgttgcca cccgatcagg ccgcagaaaa 3960 

attaagattt cgtcgtagcg cgaattctct gacgctgatt aacccgacac cctattacct 4020 

gacggtaaca gagttgaatg ccggaacccg ggttcttgaa aatgcattgg tgcctccaat 4080 

gggcgaaagc acggttaaat tgccttctga tgcaggaagc aatattactt accgaacaat 4140 

aaatgattat ggcgcactta cccccaaaat gacgggcgta atggaataac gtcgactcta 4200 



gaggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg 42 60 

gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 4320 

cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 4380 

gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 4440 

tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg 4500 

ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 4560 

gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 4 620 
gcg 



<210> 162 

<211> 42 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Oligonucleotide 



<400> 162 

aagatcttaa gctaagcttg aattctctga cgctgattaa cc 



<210> 163 

<211> 41 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Oligonucleotide 

<400> 163 

acgtaaagca tttctagacc gcggatagta atcgtgctat c 



<210> 164 

<211> 5681 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> pFIMD 



4623 



<400> 164 

tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag 60 

gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg 120 

cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 180 

caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 240 



-75- 

ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 300 

gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 360 

gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 420 

atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 480 

caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 54 0 

gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 600 

accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 660 

ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 720 

gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 780 

acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 84 0 

atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 900 

ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 960 

gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 1020 

gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 1080 

tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 1140 

taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 1200 

cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 12 60 

gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1320 

gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1380 

agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1440 

aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1500 

agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1560 

cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1620 

accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1680 

aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1740 

ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 1800 

cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 18 60 

gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 1920 

tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 1980 

agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 2040 

aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 2100 

gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 2160 



-76- 



ccccaggctt tacactttat gcttccggct 
caatttcaca caggaaacag ctatgaccat 
gattaacccg acaccctatt acctgacggt 
tgaaaatgca ttggtgcctc caatgggcga 
aagcaatatt acttaccgaa caataaatga 
cgtaatggaa taacgcaggg ggaatttttc 
gattttaagc cggaggaata atgtcatatc 
aatgcttgca tattcgtaag catcgtttgg 
gtgcttttgc cgcacaggca cctttgtcat 
tagcggatga tccccaggct gtggccgatt 
cgccagggac gtatcgcgtc gatatctatt 
tcacatttaa tacgggcgac agtgaacaag 
tcgccagtat ggggctgaat acggcttctg 
cctgtgtgcc attaaccaca atggtccagg 
agcgactgaa cctgacgatc cctcaggcat 
ctcctgagtt atgggatccc ggtattaatg 
atagtgtaca gaatcggatt gggggtaaca 
ggttaaatat tggtgcgtgg cgtttacgcg 
acagatcatc aggtagcaaa aataaatggc 
taataccgtt acgttcccgg ctgacgctgg 
atggtattaa ctttcgcggc gcacaattgg 
aaagaggatt tgccccggtg atccacggta 
aacaaaatgg gtatgacatt tataatagta 
atatctatgc cgcaggtaat agtggtgact 
gcacgcagat ttttaccgta ccctattcgt 
ctcgttattc cattacggca ggagaatacc 
gctttttcca gagtacatta ctccacggcc 
cgcaactggc ggatcgttat cgtgctttta 
tgggcgctct gtctgtggat atgacgcagg 
atgacggaca atcggtgcgt tttctctata 
ttcagttagt gggttaccgt tattcgacca 
acagtcgaat gaatggctac aacattgaaa 



cgtatgttgt gtggaattgt gagcggataa 2220 

gattacgcca agcttgaatt ctctgacgct 2280 

aacagagttg aatgccggaa cccgggttct 2340 

aagcacggtt aaattgcctt ctgatgcagg 2400 

ttatggcgca cttaccccca aaatgacggg 2460 

gcctgaataa aaagaattga ctgccggggt 2520 

tgaatttaag actttaccag cgaaacacac 2580 

ctggtttttt tgtccgactc gttgtcgcct 2640 

ctgccgacct ctattttaat ccgcgctttt 2700 

tatcgcgttt tgaaaatggg caagaattac 27 60 

tgaataatgg ttatatggca acgcgtgatg 2820 

ggattgttcc ctgcctgaca cgcgcgcaac 2880 

tcgccggtat gaatctgctg gcggatgatg 2 940 

acgctactgc gcatctggat gttggtcagc 3000 

ttatgagtaa tcgcgcgcgt ggttatattc 3060 

ccggattgct caattataat ttcagcggaa 3120 

gccattatgc atatttaaac ctacagagtg 3180 

acaataccac ctggagttat aacagtagcg 3240 

agcatatcaa tacctggctt gagcgagaca 3300 

gtgatggtta tactcagggc gatattttcg 3360 

cctcagatga caatatgtta cccgatagtc 3420 

ttgctcgtgg tactgcacag gtcactatta 3480 

cggtgccacc ggggcctttt accatcaacg 3540 

tgcaggtaac gatcaaagag gctgacggca 3600 

cagtcccgct tttgcaacgt gaagggcata 3660 

gtagtggaaa tgcgcagcag gaaaaaaccc 3720 

ttccggctgg ctggacaata tatggtggaa 3780 

atttcggtat cgggaaaaac atgggggcac 3840 

ctaattccac acttcccgat gacagtcagc 3900 

acaaatcgct caatgaatca ggcacgaata 3960 

gcggatattt taatttcgct gatacaacat 4020 

cacaggacgg agttattcag gttaagccga 4080 



-77- 

aattcaccga ctattacaac ctcgcttata acaaacgcgg gaaattacaa ctcaccgtta 4140 

ctcagcaact cgggcgcaca tcaacactgt atttgagtgg tagccatcaa acttattggg 4200 

gaacgagtaa tgtcgatgag caattccagg ctggattaaa tactgcgttc gaagatatca 4260 

actggacgct cagctatagc ctgacgaaaa acgcctggca aaaaggacgg gatcagatgt 4320 

tagcgcttaa cgtcaatatt cctttcagcc actggctgcg ttctgacagt aaatctcagt 4380 

ggcgacatgc cagtgccagc tacagcatgt cacacgatct caacggtcgg atgaccaatc 4440 

tggctggtgt atacggtacg ttgctggaag acaacaacct cagctatagc gtgcaaaccg 4500 

gctatgccgg gggaggcgat ggaaatagcg gaagtacagg ctacgccacg ctgaattatc 4560 

gcggtggtta cggcaatgcc aatatcggtt acagccatag cgatgatatt aagcagctct 4 620 

attacggagt cagcggtggg gtactggctc atgccaatgg cgtaacgctg gggcagccgt 4 680 

taaacgatac ggtggtgctt gttaaagcgc ctggcgcaaa agatgcaaaa gtcgaaaacc 4740 

agacgggggt gcgtaccgac tggcgtggtt atgccgtgct gccttatgcc actgaatatc 4800 

gggaaaatag agtggcgctg gataccaata ccctggctga taacgtcgat ttagataacg 4860 

cggttgctaa cgttgttccc actcgtgggg cgatcgtgcg agcagagttt aaagcgcgcg 4 920 

ttgggataaa actgctcatg acgctgaccc acaataataa gccgctgccg tttggggcga 4 980 

tggtgacatc agagagtagc cagagtagcg gcattgttgc ggataatggt caggtttacc 5040 

tcagcggaat gcctttagcg ggaaaagttc aggtgaaatg gggagaagag gaaaatgctc 5100 

actgtgtcgc caattatcaa ctgccaccag agagtcagca gcagttatta acccagctat 5160 

cagctgaatg tcgttaaggg ggcgtgatga gaaacaaacc tttttatctt ctgtgcgctt 5220 

ttttgtggct ggcggtgagt cacgctttgg ctgcggatag cacgattact atccgcggtc 5280 

tagaggatcc ccgggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact 5340 

gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 5400 

ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 54 60 

gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 5520 

tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc 5580 

cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 5640 

aagctgtgac cgtctccggg agctgcatgt gtcagaggtt t 5681 

<210> 165 
<211> 40 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Oligonucleotide 



<400> 165 

aattacgtga gcaagcttat gagaaacaaa cctttttatc 



40 



<210> 166 

<211> 41 

<212> DNA 

<213> Artificial Sequence 
<220> 

<223> Oligonucleotide 

<400> 166 

gactaaggcc tttctagatt attgataaac aaaagtcacg 



<210> 167 

<211> 4637 

<212> DNA 

<213> Artificial Sequence 



<220> 

<223> pFIMFGH 



<400> 167 

aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 60 

gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 120 

aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 180 

ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 240 

ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 300 

agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 360 

tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 420 

tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 480 

ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 540 

gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 600 

acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 660 

tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 720 

gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 780 

actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 840 

aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 900 

cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 960 

tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 1020 



-79- 



cgctgagata ggtgcctcac tgattaagca 
tatactttag attgatttaa aacttcattt 
ttttgataat ctcatgacca aaatccctta 
ccccgtagaa aagatcaaag gatcttcttg 
cttgcaaaca aaaaaaccac cgctaccagc 
aactcttttt ccgaaggtaa ctggcttcag 
agtgtagccg tagttaggcc accacttcaa 
tctgctaatc ctgttaccag tggctgctgc 
ggactcaaga cgatagttac cggataaggc 
cacacagccc agcttggagc gaacgaccta 
atgagaaagc gccacgcttc ccgaagggag 
ggtcggaaca ggagagcgca cgagggagct 
tcctgtcggg tttcgccacc tctgacttga 
gcggagccta tggaaaaacg ccagcaacgc 
gccttttgct cacatgttct ttcctgcgtt 
cgcctttgag tgagctgata ccgctcgccg 
gagcgaggaa gcggaagagc gcccaatacg 
tcattaatgc agctggcacg acaggtttcc 
aattaatgtg agttagctca ctcattaggc 
tcgtatgttg tgtggaattg tgagcggata 
tgattacgcc aagcttatga gaaacaaacc 
ggcggtgagt cacgctttgg ctgcggatag 
taacggctgt agtgtggccg ctgaatcaac 
ggcgaagcaa tttaacaaca ttggcgcgac 
gtcaccctgt ggtaatgccg tttctgccgt 
ccacaatgcc aacctgcttg cacttgaaaa 
acagcttctg aatgagcagc aaaatcaaat 
gtggacgacc ctgacgccgg gtaaaccaaa 
gacacaggtg cctgtcactg cggggcatat 
tcagtaactg gagatgctca tgaaatggtg 
ggcgctcgca agtgcgacga tacaggcagc 
cgtcgccaaa ccgtgtacgg tttccaccac 



ttggtaactg tcagaccaag tttactcata 1080 

ttaatttaaa aggatctagg tgaagatcct 1140 

acgtgagttt tcgttccact gagcgtcaga 1200 

agatcctttt tttctgcgcg taatctgctg 1260 

ggtggtttgt ttgccggatc aagagctacc 1320 

cagagcgcag ataccaaata ctgtccttct 1380 

gaactctgta gcaccgccta catacctcgc 1440 

cagtggcgat aagtcgtgtc ttaccgggtt 1500 

gcagcggtcg ggctgaacgg ggggttcgtg 1560 

caccgaactg agatacctac agcgtgagct 1620 

aaaggcggac aggtatccgg taagcggcag 1680 

tccaggggga aacgcctggt atctttatag 1740 

gcgtcgattt ttgtgatgct cgtcaggggg 1800 

ggccttttta cggttcctgg ccttttgctg 1860 

atcccctgat tctgtggata accgtattac 1920 

cagccgaacg accgagcgca gcgagtcagt 1980 

caaaccgcct ctccccgcgc gttggccgat 2040 

cgactggaaa gcgggcagtg agcgcaacgc 2100 

accccaggct ttacacttta tgcttccggc 2160 

acaatttcac acaggaaaca gctatgacca 2220 

tttttatctt ctgtgcgctt ttttgtggct 2280 

cacgattact atccgcggct atgtcaggga 2340 

caattttact gttgatctga tggaaaacgc 2400 

gactcctgtt gttccatttc gtattttgct 2460 

aaaggttggg tttactggcg ttgcagatag 2520 

tacggtgtca gcggcttcgg gactgggaat 2580 

accccttaat gctccatcgt ccgcgctttc 2640 

tacgctgaat ttttacgccc ggctaatggc 2700 

caatgccacg gctaccttca ctcttgaata 27 60 

caaacgtggg tatgtattgg cggcaatatt 2820 

cgatgtcacc atcacggtga acggtaaggt 2880 

caatgccacg gttgatctcg gcgatcttta 2940 



-80- 

ttctttcagt cttatgtctg ccggggcggc atcggcctgg catgatgttg cgcttgagtt 3000 

gactaattgt ccggtgggaa cgtcgagggt cactgccagc ttcagcgggg cagccgacag 3060 

taccggatat tataaaaacc aggggaccgc gcaaaacatc cagttagagc tacaggatga 3120 

cagtggcaac acattgaata ctggcgcaac caaaacagtt caggtggatg attcctcaca 3180 

atcagcgcac ttcccgttac aggtcagagc attgacagta aatggcggag ccactcaggg 324 0 

aaccattcag gcagtgatta gcatcaccta tacctacagc tgaacccgaa gagatgattg 3300 

taatgaaacg agttattacc ctgtttgctg tactgctgat gggctggtcg gtaaatgcct 3360 

ggtcattcgc ctgtaaaacc gccaatggta ccgctatccc tattggcggt ggcagcgcca 3420 

atgtttatgt aaaccttgcg cccgtcgtga atgtggggca aaacctggtc gtggatcttt 34 80 

cgacgcaaat cttttgccat aacgattatc cggaaaccat tacagactat gtcacactgc 3540 

aacgaggctc ggcttatggc ggcgtgttat ctaatttttc cgggaccgta aaatatagtg 3600 

gcagtagcta tccatttcct accaccagcg aaacgccgcg cgttgtttat aattcgagaa 3660 

cggataagcc gtggccggtg gcgctttatt tgacgcctgt gagcagtgcg ggcggggtgg 3720 

cgattaaagc tggctcatta attgccgtgc ttattttgcg acagaccaac aactataaca 3780 

gcgatgattt ccagtttgtg tggaatattt acgccaataa tgatgtggtg gtgcctactg 3840 

gcggctgcga tgtttctgct cgtgatgtca ccgttactct gccggactac cctggttcag 3900 

tgccaattcc tcttaccgtt tattgtgcga aaagccaaaa cctggggtat tacctctccg 3960 

gcacaaccgc agatgcgggc aactcgattt tcaccaatac cgcgtcgttt tcacctgcac 4020 

agggcgtcgg cgtacagttg acgcgcaacg gtacgattat tccagcgaat aacacggtat 4080 

cgttaggagc agtagggact tcggcggtga gtctgggatt aacggcaaat tatgcacgta 4140 

ccggagggca ggtgactgca gggaatgtgc aatcgattat tggcgtgact tttgtttatc 4200 

aataatctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt 4260 

cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 4320 

gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 4380 

ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca 4440 

caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc 4500 

cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct 4560 

tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca 4620 

ccgaaacgcg cgagacg 4 637 

<210> 168 
<211> 9299 



<212> DNA 

<213> Artificial Sequence 
<220> 

<223> pFIMAICDFGH 
<400> 168 

cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg 60 

tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 120 

ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 180 

aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 240 

tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 300 

atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 360 

agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 420 

tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 4 80 

tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 540 

atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 600 

ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 660 

tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 720 

acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 780 

ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 840 

aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 900 

ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 960 

cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 1020 

gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 1080 

actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 1140 

agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 1200 

cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 1260 

tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 1320 

agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 1380 

tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 1440 

acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 1500 

ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 1560 

gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 1620 



-82- 

gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 1680 

gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 1740 

tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 1800 

caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 18 60 

tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 1920 

gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 1980 

agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 2040 

ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 2100 

gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 2160 

ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 2220 

atgaccatga ttacgccaag cttataatag aaatagtttt ttgaaaggaa agcagcatga 2280 

aaattaaaac tctggcaatc gttgttctgt cggctctgtc cctcagttct acagcggctc 2340 

tggccgctgc cacgacggtt aatggtggga ccgttcactt taaaggggaa gttgttaacg 24 00 

ccgcttgcgc agttgatgca ggctctgttg atcaaaccgt tcagttagga caggttcgta 24 60 

ccgcatcgct ggcacaggaa ggagcaacca gttctgctgt cggttttaac attcagctga 2520 

atgattgcga taccaatgtt gcatctaaag ccgctgttgc ctttttaggt acggcgattg 2580 

atgcgggtca taccaacgtt ctggctctgc agagttcagc tgcgggtagc gcaacaaacg 2640 

ttggtgtgca gatcctggac agaacgggtg ctgcgctgac gctggatggt gcgacattta 2700 

gttcagaaac aaccctgaat aacggaacca ataccattcc gttccaggcg cgttattttg 2760 

caaccggggc cgcaaccccg ggtgctgcta atgcggatgc gaccttcaag gttcagtatc 2820 

aataacctac ccaggttcag ggacgtcatt acgggcaggg atgcccaccc ttgtgcgata 2880 

aaaataacga tgaaaaggaa gagattattt ctattagcgt cgttgctgcc aatgtttgct 2940 

ctggccggaa ataaatggaa taccacgttg cccggcggaa atatgcaatt tcagggcgtc 3000 

attattgcgg aaacttgccg gattgaagcc ggtgataaac aaatgacggt caatatgggg 3060 

caaatcagca gtaaccggtt tcatgcggtt ggggaagata gcgcaccggt gccttttgtt 3120 

attcatttac gggaatgtag cacggtggtg agtgaacgtg taggtgtggc gtttcacggt 3180 

gtcgcggatg gtaaaaatcc ggatgtgctt tccgtgggag aggggccagg gatagccacc 3240 

aatattggcg tagcgttgtt tgatgatgaa ggaaacctcg taccgattaa tcgtcctcca 3300 

gcaaactgga aacggcttta ttcaggctct acttcgctac atttcatcgc caaatatcgt 3360 

gctaccgggc gtcgggttac tggcggcatc gccaatgccc aggcctggtt ctctttaacc 3420 

tatcagtaat tgttcagcag ataatgtgat aacaggaaca ggacagtgag taataaaaac 3480 

gtcaatgtaa ggaaatcgca ggaaataaca ttctgcttgc tggcaggtat cctgatgttc 354 0 



atggcaatga 
atttatccgg 
acctatttaa 
gtgacgcctc 
gcaacaaata 
attccgtcaa 
cgcattaaac 
aaattaagat 
ctgacggtaa 
atgggcgaaa 
ataaatgatt 
aatttttcgc 
gtcatatctg 
tcgtttggct 
tttgtcatct 
ggccgattta 
tatctatttg 
tgaacaaggg 
ggcttctgtc 
ggtccaggac 
tcaggcattt 
tattaatgcc 
gggtaacagc 
tttacgcgac 
taaatggcag 
gacgctgggt 
acaattggcc 
ccacggtatt 
taatagtacg 
tggtgacttg 
ctattcgtca 
agaataccgt 



tggttgccgg acgcgctgaa 
cagggcaaaa acaagagcaa 
ttcaatcatg ggtggaaaat 
ctctgtttgc gatgaaggga 
accaattgcc acaggaccgg 
tggataaatc aaaattgact 
tgtactatcg cccggctaaa 
ttcgtcgtag cgcgaattct 
cagagttgaa tgccggaacc 
gcacggttaa attgccttct 
atggcgcact tacccccaaa 
ctgaataaaa agaattgact 
aatttaagac tttaccagcg 
ggtttttttg tccgactcgt 
gccgacctct attttaatcc 
tcgcgttttg aaaatgggca 
aataatggtt atatggcaac 
attgttccct gcctgacacg 
gccggtatga atctgctggc 
gctactgcgc atctggatgt 
atgagtaatc gcgcgcgtgg 
ggattgctca attataattt 
cattatgcat atttaaacct 
aataccacct ggagttataa 
catatcaata cctggcttga 
gatggttata ctcagggcga 
tcagatgaca atatgttacc 
gctcgtggta ctgcacaggt 
gtgccaccgg ggccttttac 
caggtaacga tcaaagaggc 
gtcccgcttt tgcaacgtga 
agtggaaatg cgcagcagga 



-83- 

gcgggagtgg ccttaggtgc 
cttgccgtga caaataatga 
gccgatggtg taaaggatgg 
aaaaaagaga ataccttacg 
gaaagtttat tctggatgaa 
gagaatacgc tacagctcgc 
ttagcgttgc cacccgatca 
ctgacgctga ttaacccgac 
cgggttcttg aaaatgcatt 
gatgcaggaa gcaatattac 
atgacgggcg taatggaata 
gccggggtga ttttaagccg 
aaacacacaa tgcttgcata 
tgtcgcctgt gcttttgccg 
gcgcttttta gcggatgatc 
agaattaccg ccagggacgt 
gcgtgatgtc acatttaata 
cgcgcaactc gccagtatgg 
ggatgatgcc tgtgtgccat 
tggtcagcag cgactgaacc 
ttatattcct cctgagttat 
cagcggaaat agtgtacaga 
acagagtggg ttaaatattg 
cagtagcgac agatcatcag 
gcgagacata ataccgttac 
tattttcgat ggtattaact 
cgatagtcaa agaggatttg 
cactattaaa caaaatgggt 
catcaacgat atctatgccg 
tgacggcagc acgcagattt 
agggcatact cgttattcca 
aaaaacccgc tttttccaga 



gactcgcgta 


3600 


tgaaaatagt 


3660 


tcgttttatc 


3720 


tattcttgat 


3780 


cgttaaagcg 


3840 


aattatcagc 


3900 


ggccgcagaa 


3960 


accctattac 


4020 


ggtgcctcca 


4080 


ttaccgaaca 


4140 


acgcaggggg 


4200 


gaggaataat 


4260 


ttcgtaagca 


4320 


cacaggcacc 


4380 


cccaggctgt 


4440 


atcgcgtcga 


4500 


cgggcgacag 


4560 


ggctgaatac 


4620 


taaccacaat 


4680 


tgacgatccc 


4740 


gggatcccgg 


4800 


atcggattgg 


4860 


gtgcgtggcg 


4920 


gtagcaaaaa 


4 980 


gttcccggct 


5040 


ttcgcggcgc 


5100 


ccccggtgat 


5160 


atgacattta 


5220 


caggtaatag 


5280 


ttaccgtacc 


5340 


ttacggcagg 


5400 


gtacattact 


5460 



-84- 

ccacggcctt ccggctggct ggacaatata tggtggaacg caactggcgg atcgttatcg 5520 

tgcttttaat ttcggtatcg ggaaaaacat gggggcactg ggcgctctgt ctgtggatat 5580 

gacgcaggct aattccacac ttcccgatga cagtcagcat gacggacaat cggtgcgttt 5640 

tctctataac aaatcgctca atgaatcagg cacgaatatt cagttagtgg gttaccgtta 5700 

ttcgaccagc ggatatttta atttcgctga tacaacatac agtcgaatga atggctacaa 5760 

cattgaaaca caggacggag ttattcaggt taagccgaaa ttcaccgact attacaacct 5820 

cgcttataac aaacgcggga aattacaact caccgttact cagcaactcg ggcgcacatc 5880 

aacactgtat ttgagtggta gccatcaaac ttattgggga acgagtaatg tcgatgagca 5940 

attccaggct ggattaaata ctgcgttcga agatatcaac tggacgctca gctatagcct 6000 

gacgaaaaac gcctggcaaa aaggacggga tcagatgtta gcgcttaacg tcaatattcc 6060 

tttcagccac tggctgcgtt ctgacagtaa atctcagtgg cgacatgcca gtgccagcta 6120 

cagcatgtca cacgatctca acggtcggat gaccaatctg gctggtgtat acggtacgtt 6180 

gctggaagac aacaacctca gctatagcgt gcaaaccggc tatgccgggg gaggcgatgg 6240 

aaatagcgga agtacaggct acgccacgct gaattatcgc ggtggttacg gcaatgccaa 6300 

tatcggttac agccatagcg atgatattaa gcagctctat tacggagtca gcggtggggt 6360 

actggctcat gccaatggcg taacgctggg gcagccgtta aacgatacgg tggtgcttgt 6420 

taaagcgcct ggcgcaaaag atgcaaaagt cgaaaaccag acgggggtgc gtaccgactg 6480 

gcgtggttat gccgtgctgc cttatgccac tgaatatcgg gaaaatagag tggcgctgga 6540 

taccaatacc ctggctgata acgtcgattt agataacgcg gttgctaacg ttgttcccac 6600 

tcgtggggcg atcgtgcgag cagagtttaa agcgcgcgtt gggataaaac tgctcatgac 6660 

gctgacccac aataataagc cgctgccgtt tggggcgatg gtgacatcag agagtagcca 6720 

gagtagcggc attgttgcgg ataatggtca ggtttacctc agcggaatgc ctttagcggg 6780 

aaaagttcag gtgaaatggg gagaagagga aaatgctcac tgtgtcgcca attatcaact 684 0 

gccaccagag agtcagcagc agttattaac ccagctatca gctgaatgtc gttaaggggg 6900 

cgtgatgaga aacaaacctt tttatcttct gtgcgctttt ttgtggctgg cggtgagtca 6960 

cgctttggct gcggatagca cgattactat ccgcggctat gtcagggata acggctgtag 7020 

tgtggccgct gaatcaacca attttactgt tgatctgatg gaaaacgcgg cgaagcaatt 7080 

taacaacatt ggcgcgacga ctcctgttgt tccatttcgt attttgctgt caccctgtgg 7140 

taatgccgtt tctgccgtaa aggttgggtt tactggcgtt gcagatagcc acaatgccaa 7200 

cctgcttgca cttgaaaata cggtgtcagc ggcttcggga ctgggaatac agcttctgaa 72 60 

tgagcagcaa aatcaaatac cccttaatgc tccatcgtcc gcgctttcgt ggacgaccct 7320 

gacgccgggt aaaccaaata cgctgaattt ttacgcccgg ctaatggcga cacaggtgcc 7380 



-85- 

tgtcactgcg gggcatatca atgccacggc taccttcact cttgaatatc agtaactgga 7440 

gatgctcatg aaatggtgca aacgtgggta tgtattggcg gcaatattgg cgctcgcaag 7500 

tgcgacgata caggcagccg atgtcaccat cacggtgaac ggtaaggtcg tcgccaaacc 7560 

gtgtacggtt tccaccacca atgccacggt tgatctcggc gatctttatt ctttcagtct 7620 

tatgtctgcc ggggcggcat cggcctggca tgatgttgcg cttgagttga ctaattgtcc 7 680 

ggtgggaacg tcgagggtca ctgccagctt cagcggggca gccgacagta ccggatatta 7740 

taaaaaccag gggaccgcgc aaaacatcca gttagagcta caggatgaca gtggcaacac 7800 

attgaatact ggcgcaacca aaacagttca ggtggatgat tcctcacaat cagcgcactt 7860 

cccgttacag gtcagagcat tgacagtaaa tggcggagcc actcagggaa ccattcaggc 7 920 

agtgattagc atcacctata cctacagctg aacccgaaga gatgattgta atgaaacgag 7 980 

ttattaccct gtttgctgta ctgctgatgg gctggtcggt aaatgcctgg tcattcgcct 8040 

gtaaaaccgc caatggtacc gctatcccta ttggcggtgg cagcgccaat gtttatgtaa 8100 

accttgcgcc cgtcgtgaat gtggggcaaa acctggtcgt ggatctttcg acgcaaatct 8160 

tttgccataa cgattatccg gaaaccatta cagactatgt cacactgcaa cgaggctcgg 8220 

cttatggcgg cgtgttatct aatttttccg ggaccgtaaa atatagtggc agtagctatc 8280 

catttcctac caccagcgaa acgccgcgcg ttgtttataa ttcgagaacg gataagccgt 8340 

ggccggtggc gctttatttg acgcctgtga gcagtgcggg cggggtggcg attaaagctg 84 00 

gctcattaat tgccgtgctt attttgcgac agaccaacaa ctataacagc gatgatttcc 8460 

agtttgtgtg gaatatttac gccaataatg atgtggtggt gcctactggc ggctgcgatg 8520 

tttctgctcg tgatgtcacc gttactctgc cggactaccc tggttcagtg ccaattcctc 8580 

ttaccgttta ttgtgcgaaa agccaaaacc tggggtatta cctctccggc acaaccgcag 8640 

atgcgggcaa ctcgattttc accaataccg cgtcgttttc acctgcacag ggcgtcggcg 8700 

tacagttgac gcgcaacggt acgattattc cagcgaataa cacggtatcg ttaggagcag 87 60 

tagggacttc ggcggtgagt ctgggattaa cggcaaatta tgcacgtacc ggagggcagg 8820 

tgactgcagg gaatgtgcaa tcgattattg gcgtgacttt tgtttatcaa taatctagaa 8880 

ggatccccgg gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc gtgactggga 8940 

aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 9000 

taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 9060 

atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg 9120 

gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc 9180 

aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 9240 

tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcg 9299 



-86- 



<210> 169 
<211> 8464 
<212> DNA 

<213> Artificial Sequence 
<220> 

<223> pFIMAICDFG 
<400> 169 

cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg 60 

tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 120 

ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 180 

aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 240 

tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 300 

atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 360 

agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 420 

tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 480 

tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 540 

atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 600 

ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 660 

tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 720 

acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 780 

ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 840 

aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 900 

ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 960 

cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 1020 

gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 1080 

actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 1140 

agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 1200 

cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 1260 

tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 1320 

agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 1380 

tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 1440 



acctcgctct 
ccgggttgga 
gttcgtgcac 
gtgagctatg 
gcggcagggt 
tttatagtcc 
caggggggcg 
tttgctggcc 
gtattaccgc 
agtcagtgag 
ggccgattca 
gcaacgcaat 
ttccggctcg 
atgaccatga 
aaattaaaac 
tggccgctgc 
ccgcttgcgc 
ccgcatcgct 
atgattgcga 
atgcgggtca 
ttggtgtgca 
gttcagaaac 
caaccggggc 
aataacctac 
aaaataacga 
ctggccggaa 
attattgcgg 
caaatcagca 
attcatttac 
gtcgcggatg 
aatattggcg 
gcaaactgga 



gctaatcctg ttaccagtgg 
ctcaagacga tagttaccgg 
acagcccagc ttggagcgaa 
agaaagcgcc acgcttcccg 
cggaacagga gagcgcacga 
tgtcgggttt cgccacctct 
gagcctatgg aaaaacgcca 
ttttgctcac atgttctttc 
ctttgagtga gctgataccg 
cgaggaagcg gaagagcgcc 
ttaatgcagc tggcacgaca 
taatgtgagt tagctcactc 
tatgttgtgt ggaattgtga 
ttacgccaag cttataatag 
tctggcaatc gttgttctgt 
cacgacggtt aatggtggga 
agttgatgca ggctctgttg 
ggcacaggaa ggagcaacca 
taccaatgtt gcatctaaag 
taccaacgtt ctggctctgc 
gatcctggac agaacgggtg 
aaccctgaat aacggaacca 
cgcaaccccg ggtgctgcta 
ccaggttcag ggacgtcatt 
tgaaaaggaa gagattattt 
ataaatggaa taccacgttg 
aaacttgccg gattgaagcc 
gtaaccggtt tcatgcggtt 
gggaatgtag cacggtggtg 
gtaaaaatcc ggatgtgctt 
tagcgttgtt tgatgatgaa 
aacggcttta ttcaggctct 



-87- 

ctgctgccag tggcgataag 
ataaggcgca gcggtcgggc 
cgacctacac cgaactgaga 
aagggagaaa ggcggacagg 
gggagcttcc agggggaaac 
gacttgagcg tcgatttttg 
gcaacgcggc ctttttacgg 
ctgcgttatc ccctgattct 
ctcgccgcag ccgaacgacc 
caatacgcaa accgcctctc 
ggtttcccga ctggaaagcg 
attaggcacc ccaggcttta 
gcggataaca atttcacaca 
aaatagtttt ttgaaaggaa 
cggctctgtc cctcagttct 
ccgttcactt taaaggggaa 
atcaaaccgt tcagttagga 
gttctgctgt cggttttaac 
ccgctgttgc ctttttaggt 
agagttcagc tgcgggtagc 
ctgcgctgac gctggatggt 
ataccattcc gttccaggcg 
atgcggatgc gaccttcaag 
acgggcaggg atgcccaccc 
ctattagcgt cgttgctgcc 
cccggcggaa atatgcaatt 
ggtgataaac aaatgacggt 
ggggaagata gcgcaccggt 
agtgaacgtg taggtgtggc 
tccgtgggag aggggccagg 
ggaaacctcg taccgattaa 
acttcgctac atttcatcgc 



tcgtgtctta 


1500 


tgaacggggg 


1560 


tacctacagc 


1620 


tatccggtaa 


1680 


gcctggtatc 


1740 


tgatgctcgt 


1800 


ttcctggcct 


1860 


gtggataacc 


1920 


gagcgcagcg 


1980 


cccgcgcgtt 


2040 


ggcagtgagc 


2100 


cactttatgc 


2160 


ggaaacagct 


2220 


agcagcatga 


2280 


acagcggctc 


2340 


gttgttaacg 


2400 


caggttcgta 


2460 


attcagctga 


2520 


acggcgattg 


2580 


gcaacaaacg 


2640 


gcgacattta 


2700 


cgttattttg 


2760 


gttcagtatc 


2820 


ttgtgcgata 


2880 


aatgtttgct 


2940 


tcagggcgtc 


3000 


caatatgggg 


3060 


gccttttgtt 


3120 


gtttcacggt 


3180 


gatagccacc 


3240 


tcgtcctcca 


3300 


caaatatcgt 


3360 



-88- 

gctaccgggc gtcgggttac tggcggcatc gccaatgccc aggcctggtt ctctttaacc 3420 

tatcagtaat tgttcagcag ataatgtgat aacaggaaca ggacagtgag taataaaaac 3480 

gtcaatgtaa ggaaatcgca ggaaataaca ttctgcttgc tggcaggtat cctgatgttc 3540 

atggcaatga tggttgccgg acgcgctgaa gcgggagtgg ccttaggtgc gactcgcgta 3600 

atttatccgg cagggcaaaa acaagagcaa cttgccgtga c'aaataatga tgaaaatagt 3660 

acctatttaa ttcaatcatg ggtggaaaat gccgatggtg taaaggatgg tcgttttatc 3720 

gtgacgcctc ctctgtttgc gatgaaggga aaaaaagaga ataccttacg tattcttgat 3780 

gcaacaaata accaattgcc acaggaccgg gaaagtttat tctggatgaa cgttaaagcg 3840 

attccgtcaa tggataaatc aaaattgact gagaatacgc tacagctcgc aattatcagc 3900 

cgcattaaac tgtactatcg cccggctaaa ttagcgttgc cacccgatca ggccgcagaa 3960 

aaattaagat ttcgtcgtag cgcgaattct ctgacgctga ttaacccgac accctattac 4020 

ctgacggtaa cagagttgaa tgccggaacc cgggttcttg aaaatgcatt ggtgcctcca 4080 

atgggcgaaa gcacggttaa attgccttct gatgcaggaa gcaatattac ttaccgaaca 4140 

ataaatgatt atggcgcact tacccccaaa atgacgggcg taatggaata acgcaggggg 4200 

aatttttcgc ctgaataaaa agaattgact gccggggtga ttttaagccg gaggaataat 4260 

gtcatatctg aatttaagac tttaccagcg aaacacacaa tgcttgcata ttcgtaagca 4320 

tcgtttggct ggtttttttg tccgactcgt tgtcgcctgt gcttttgccg cacaggcacc 4380 

tttgtcatct gccgacctct attttaatcc gcgcttttta gcggatgatc cccaggctgt 4440 

ggccgattta tcgcgttttg aaaatgggca agaattaccg ccagggacgt atcgcgtcga 4500 

tatctatttg aataatggtt atatggcaac gcgtgatgtc acatttaata cgggcgacag 4560 

tgaacaaggg attgttccct gcctgacacg cgcgcaactc gccagtatgg ggctgaatac 4 620 

ggcttctgtc gccggtatga atctgctggc ggatgatgcc tgtgtgccat taaccacaat 4680 

ggtccaggac gctactgcgc atctggatgt tggtcagcag cgactgaacc tgacgatccc 4740 

tcaggcattt atgagtaatc gcgcgcgtgg ttatattcct cctgagttat gggatcccgg 4800 

tattaatgcc ggattgctca attataattt cagcggaaat agtgtacaga atcggattgg 4860 

gggtaacagc cattatgcat atttaaacct acagagtggg ttaaatattg gtgcgtggcg 4920 

tttacgcgac aataccacct ggagttataa cagtagcgac agatcatcag gtagcaaaaa 4980 

taaatggcag catatcaata cctggcttga gcgagacata ataccgttac gttcccggct 5040 
gacgctgggt gatggttata ctcagggcga tattttcgat ggtattaact ttcgcggcgc 5100 
acaattggcc tcagatgaca atatgttacc cgatagtcaa agaggatttg ccccggtgat 5160 
ccacggtatt gctcgtggta ctgcacaggt cactattaaa caaaatgggt atgacattta 5220 
taatagtacg gtgccaccgg ggccttttac catcaacgat atctatgccg caggtaatag 5280 



-89- 

tggtgacttg caggtaacga tcaaagaggc tgacggcagc acgcagattt ttaccgtacc 5340 

ctattcgtca gtcccgcttt tgcaacgtga agggcatact cgttattcca ttacggcagg 54 00 

agaataccgt agtggaaatg cgcagcagga aaaaacccgc tttttccaga gtacattact 5460 

ccacggcctt ccggctggct ggacaatata tggtggaacg caactggcgg atcgttatcg 5520 

tgcttttaat ttcggtatcg ggaaaaacat gggggcactg ggcgctctgt ctgtggatat 5580 

gacgcaggct aattccacac ttcccgatga cagtcagcat gacggacaat cggtgcgttt 5640 

tctctataac aaatcgctca atgaatcagg cacgaatatt cagttagtgg gttaccgtta 5700 

ttcgaccagc ggatatttta atttcgctga tacaacatac agtcgaatga atggctacaa 57 60 

cattgaaaca caggacggag ttattcaggt taagccgaaa ttcaccgact attacaacct 5820 

cgcttataac aaacgcggga aattacaact caccgttact cagcaactcg ggcgcacatc 5880 

aacactgtat ttgagtggta gccatcaaac ttattgggga acgagtaatg tcgatgagca 5940 

attccaggct ggattaaata ctgcgttcga agatatcaac tggacgctca gctatagcct 6000 

gacgaaaaac gcctggcaaa aaggacggga tcagatgtta gcgcttaacg tcaatattcc 6060 

tttcagccac tggctgcgtt ctgacagtaa atctcagtgg cgacatgcca gtgccagcta 6120 

cagcatgtca cacgatctca acggtcggat gaccaatctg gctggtgtat acggtacgtt 6180 

gctggaagac aacaacctca gctatagcgt gcaaaccggc tatgccgggg gaggcgatgg 6240 

aaatagcgga agtacaggct acgccacgct gaattatcgc ggtggttacg gcaatgccaa 6300 

tatcggttac agccatagcg atgatattaa gcagctctat tacggagtca gcggtggggt 6360 

actggctcat gccaatggcg taacgctggg gcagccgtta aacgatacgg tggtgcttgt 6420 

taaagcgcct ggcgcaaaag atgcaaaagt cgaaaaccag acgggggtgc gtaccgactg 64 80 

gcgtggttat gccgtgctgc cttatgccac tgaatatcgg gaaaatagag tggcgctgga 6540 

taccaatacc ctggctgata acgtcgattt agataacgcg gttgctaacg ttgttcccac 6600 

tcgtggggcg atcgtgcgag cagagtttaa agcgcgcgtt gggataaaac tgctcatgac 6660 

gctgacccac aataataagc cgctgccgtt tggggcgatg gtgacatcag agagtagcca 6720 

gagtagcggc attgttgcgg ataatggtca ggtttacctc agcggaatgc ctttagcggg 6780 

aaaagttcag gtgaaatggg gagaagagga aaatgctcac tgtgtcgcca attatcaact 6840 

gccaccagag agtcagcagc agttattaac ccagctatca gctgaatgtc gttaaggggg 6900 

cgtgatgaga aacaaacctt tttatcttct gtgcgctttt ttgtggctgg cggtgagtca 6960 

cgctttggct gcggatagca cgattactat ccgcggctat gtcagggata acggctgtag 7020 

tgtggccgct gaatcaacca attttactgt tgatctgatg gaaaacgcgg cgaagcaatt 7080 

taacaacatt ggcgcgacga ctcctgttgt tccatttcgt attttgctgt caccctgtgg 7140 

taatgccgtt tctgccgtaa aggttgggtt tactggcgtt gcagatagcc acaatgccaa 7200 



cctgcttgca cttgaaaata cggtgtcagc ggcttcggga ctgggaatac agcttctgaa 7260 

tgagcagcaa aatcaaatac cccttaatgc tccatcgtcc gcgctttcgt ggacgaccct 7320 

gacgccgggt aaaccaaata cgctgaattt ttacgcccgg ctaatggcga cacaggtgcc 7380 

tgtcactgcg gggcatatca atgccacggc taccttcact cttgaatatc agtaactgga 7440 

gatgctcatg aaatggtgca aacgtgggta tgtattggcg gcaatattgg cgctcgcaag 7500 

tgcgacgata caggcagccg atgtcaccat cacggtgaac ggtaaggtcg tcgccaaacc 7560 

gtgtacggtt tccaccacca atgccacggt tgatctcggc gatctttatt ctttcagtct 7620 

tatgtctgcc ggggcggcat cggcctggca tgatgttgcg cttgagttga ctaattgtcc 7 680 

ggtgggaacg tcgagggtca ctgccagctt cagcggggca gccgacagta ccggatatta 7740 

taaaaaccag gggaccgcgc aaaacatcca gttagagcta caggatgaca gtggcaacac 7800 

attgaatact ggcgcaacca aaacagttca ggtggatgat tcctcacaat cagcgcactt 7860 

cccgttacag gtcagagcat tgacagtaaa tggcggagcc actcagggaa ccattcaggc 7920 
agtgattagc atcacctata cctacagctg aacccgaaga gatgattgta atgaaacgag 
ttattaccct gtttgctgta ctgctgatgg gctggtcggt aaatgcctgg tcattcgcct 
gtaaaaccgc caatggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac 
tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 

tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 8220 
ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc 
atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac 
ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga 

caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa 8460 

cgcg 8464 



<210> 170 

<211> 27 

<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Synthetic M2 Peptide 

<400> 170 

Ser Leu Leu Thr Glu Val Glu Thr Pro He Arg Asn Glu Trp Gly Cys 



7980 
8040 
8100 
8160 



8280 
8340 
8400 



Arg Cys Asn Gly Ser Ser Asp Gly Gly Gly Cys 



<210> 171 



<211> 97 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> Matrix protein M2 
<400> 171 

Met Ser Leu Leu Thr Glu Val Glu Thr Pro He Arg Asn Glu Trp Gly 
15 10 15 

Cvs Arq Cys Asn Gly Ser Ser Asp Pro Leu Ala He Ala Ala Asn He 
20 25 30 

He Gly He Leu His Leu He Leu Trp He Leu Asp Arg Leu Phe Phe 
35 40 45 

Lys Cys He Tyr Arg Arg Phe Lys Tyr Gly Leu Lys Gly Gly Pro Ser 
50 55 60 

Thr Glu Gly Val Pro Lys Ser Met Arg Glu Glu Tyr Arg Lys Glu Gin 
65 70 75 80 

Gin Ser Ala Val Asp Ala Asp Asp Gly His Phe Val Ser He Glu Leu 



<210> 172 

<211> 770 

<212> PRT 

<213> Homo Sapiens 

<400> 172 

Met Leu Pro Gly Leu Ala Leu Leu Leu Leu Ala Ala Trp Thr Ala Arg 
15 10 15 

Ala Leu Glu Val Pro Thr Asp Gly Asn Ala Gly Leu Leu Ala Glu Pro 
20 25 30 

Gin He Ala Met Phe Cys Gly Arg Leu Asn Met His Met Asn Val Gin 
35 40 45 

Asn Gly Lys Trp Asp Ser Asp Pro Ser Gly Thr Lys Thr Cys He Asp 
50 55 60 

Thr Lys Glu Gly He Leu Gin Tyr Cys Gin Glu Val Tyr Pro Glu Leu 
65 70 75 80 

Gin He Thr Asn Val Val Glu Ala Asn Gin Pro Val Thr He Gin Asn 
85 90 95 

Trp Cys Lys Arg Gly Arg Lys Gin Cys Lys Thr His Pro His Phe Val 
100 105 HO 



-92- 



Ile Pro Tyr Arg Cys Leu Val Gly Glu Phe Val Ser Asp Ala Leu Leu 
115 120 125 

Val Pro Asp Lys Cys Lys Phe Leu His Gin Glu Arg Met Asp Val Cys 
130 135 140 

Glu Thr His Leu His Trp His Thr Val Ala Lys Glu Thr Cys Ser Glu 
145 150 155 160 

Lys Ser Thr Asn Leu His Asp Tyr Gly Met Leu Leu Pro Cys Gly He 
165 170 175 

Asp Lys Phe Arg Gly Val Glu Phe Val Cys Cys Pro Leu Ala Glu Glu 
180 185 190 

Ser Asp Asn Val Asp Ser Ala Asp Ala Glu Glu Asp Asp Ser Asp Val 
195 200 205 

Trp Trp Gly Gly Ala Asp Thr Asp Tyr Ala Asp Gly Ser Glu Asp Lys 
210 215 220 

Val Val Glu Val Ala Glu Glu Glu Glu Val Ala Glu Val Glu Glu Glu 
225 230 235 240 

Glu Ala Asp Asp Asp Glu Asp Asp Glu Asp Gly Asp Glu Val Glu Glu 
245 250 255 

Glu Ala Glu Glu Pro Tyr Glu Glu Ala Thr Glu Arg Thr Thr Ser He 
260 265 270 

Ala Thr Thr Thr Thr Thr Thr Thr Glu Ser Val Glu Glu Val Val Arg 
275 280 285 

Glu Val Cys Ser Glu Gin Ala Glu Thr Gly Pro Cys Arg Ala Met He 
290 295 300 

Ser Arg Trp Tyr Phe Asp Val Thr Glu Gly Lys Cys Ala Pro Phe Phe 
305 310 315 320 

Tyr Gly Gly Cys Gly Gly Asn Arg Asn Asn Phe Asp Thr Glu Glu Tyr 
325 330 335 

Cys Met Ala Val Cys Gly Ser Ala Met Ser Gin Ser Leu Leu Lys Thr 
340 345 350 

Thr Gin Glu Pro Leu Ala Arg Asp Pro Val Lys Leu Pro Thr Thr Ala 
355 360 365 

Ala Ser Thr Pro Asp Ala Val Asp Lys Tyr Leu Glu Thr Pro Gly Asp 
370 375 380 

Glu Asn Glu His Ala His Phe Gin Lys Ala Lys Glu Arg Leu Glu Ala 
385 390 395 400 

Lys His Arg Glu Arg Met Ser Gin Val Met Arg Glu Trp Glu Glu Ala 
405 410 415 

Glu Arg Gin Ala Lys Asn Leu Pro Lys Ala Asp Lys Lys Ala Val He 
420 425 430 

Gin His Phe Gin Glu Lys Val Glu Ser Leu Glu Gin Glu Ala Ala Asn 
435 440 445 



-93- 



Glu Arg Gin Gin Leu Val Glu Thr His Met Ala Arg Val Glu Ala Met 
450 455 460 

Leu Asn Asp Arg Arg Arg Leu Ala Leu Glu Asn Tyr He Thr Ala Leu 
465 470 475 480 

Gin Ala Val Pro Pro Arg Pro Arg His Val Phe Asn Met Leu Lys Lys 
485 490 495 

Tyr Val Arg Ala Glu Gin Lys Asp Arg Gin His Thr Leu Lys His Phe 
500 505 510 

Glu His Val Arg Met Val Asp Pro Lys Lys Ala Ala Gin He Arg Ser 
515 520 525 

Gin Val Met Thr His Leu Arg Val He Tyr Glu Arg Met Asn Gin Ser 
530 535 540 

Leu Ser Leu Leu Tyr Asn Val Pro Ala Val Ala Glu Glu He Gin Asp 
545 550 555 560 

Glu Val Asp Glu Leu Leu Gin Lys Glu Gin Asn Tyr Ser Asp Asp Val 
565 570 575 

Leu Ala Asn Met He Ser Glu Pro Arg He Ser Tyr Gly Asn Asp Ala 
580 585 590 

Leu Met Pro Ser Leu Thr Glu Thr Lys Thr Thr Val Glu Leu Leu Pro 
595 600 605 

Val Asn Gly Glu Phe Ser Leu Asp Asp Leu Gin Pro Trp His Ser Phe 
610 615 620 

Gly Ala Asp Ser Val Pro Ala Asn Thr Glu Asn Glu Val Glu Pro Val 
625 630 635 640 

Asp Ala Arg Pro Ala Ala Asp Arg Gly Leu Thr Thr Arg Pro Gly Ser 
645 650 655 

Gly Leu Thr Asn He Lys Thr Glu Glu He Ser Glu Val Lys Met Asp 
660 665 670 

Ala Glu Phe Arg His Asp Ser Gly Tyr Glu Val His His Gin Lys Leu 
675 680 685 

Val Phe Phe Ala Glu Asp Val Gly Ser Asn Lys Gly Ala He He Gly 
690 695 700 

Leu Met Val Gly Gly Val Val He Ala Thr Val He Val He Thr Leu 
705 710 715 720 

Val Met Leu Lys Lys Lys Gin Tyr Thr Ser He His His Gly Val Val 
725 730 735 

Glu Val Asp Ala Ala Val Thr Pro Glu Glu Arg His Leu Ser Lys Met 
740 745 750 

Gin Gin Asn Gly Tyr Glu Asn Pro Thr Tyr Lys Phe Phe Glu Gin Met 
755 760 765 



Gin Asn 
770 



-94- 



<210> 173 

<211> 82 

<212> PRT 

<213> Homo Sapiens 

<400> 173 

Gly Ser Gly Leu Thr Asn He Lys Thr Glu Glu He Ser Glu Val Lys 
15 10 15 

Met Asp Ala Glu Phe Arg His Asp Ser Gly Tyr Glu Val His His Gin 
20 25 30 

Lys Leu Val Phe Phe Ala Glu Asp Val Gly Ser Asn Lys Gly Ala He 
35 40 45 

He Gly Leu Met Val Gly Gly Val Val He Ala Thr Val He He He 
50 55 60 

Thr Leu Val Met Leu Lys Lys Gin Tyr Thr Ser Asn His His Gly Val 
65 70 75 80 

Val Glu 

<210> 174 
<211> 42 
<212> PRT 
<213> Unknown 
<220> 

<223> Amyloid Beta Peptide 
<400> 174 

Asp Ala Glu Phe Arg His Asp Ser Gly Tyr Glu Val His His Gin Lys 
15 10 15 

Leu Val Phe Phe Ala Glu Asp Val Gly Ser Asn Lys Gly Ala He He 
20 25 30 

Gly Leu Met Val Gly Gly Val Val He Ala 
35 40 

<210> 175 

<211> 12 

<212> PRT 

<213> Artificial Sequence 



-95- 



<220> 

<223> p33 peptide 
<400> 175 

Cys Gly Gly Lys Ala Val Tyr Asn Phe Ala Thr Met 
1 5 10 

<210> 176 

<211> 37 

<212> PRT 

<213> Artificial Sequence 
<220> 

<223> DP178c peptide 
<400> 176 

Cys Tyr Thr Ser Leu He His Ser Leu He Glu Glu Ser Gin Asn Gin 
15 10 15 

Gin Glu Lys Asn Glu Gin Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser 
20 25 30 

Leu Trp Asn Trp Phe 
35 

<210> 177 
<211> 6 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> c-terminal linker 
<400> 177 

Gly Ser Gly Gly Cys Gly 
1 5 

<210> 178 

<211> 65 



-96- 



<212> PRT 

<213> Artificial Sequence 
<220> 

<223> GRA2 
<400> 178 

Lys Glu Ala Ala Gly Arg Gly Met Val Thr Val Gly Lys Lys Leu Ala 
15 10 15 

Asn Val Glu Ser Asp Arg Ser Thr Thr Thr Thr Gin Ala Pro Asp Ser 
20 25 30 

Pro Asn Gly Leu Ala Glu Thr Glu Val Pro Val Glu Pro Gin Gin Arg 
35 40 45 

Ala Ala His Val Pro Val Pro Asp Phe Ser Gin Gly Ser Gly Gly Cys 
50 55 60 

Gly 
65 

<210> 179 
<211> 18 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> D2 peptide 
<400> 179 

Cys Gly Gly Thr Ser Asn Gly Ser Asn Pro Ser Thr Ser Tyr Gly Phe 
15 10 15 

Ala Asn 

<210> 180 

<211> 18 

<212> PRT 

<213> Artificial Sequence 



-97- 



<220> 

<223> B2 peptide 
<400> 180 

Cys Gly Gly Asp He Ser Asn Gly Tyr Gly Ala Ser Tyr Gly Asp Asn 
15 10 15 

Asp He 

<210> 181 
<211> 14 
<212> PRT 

<213> Artificial Sequence 
<220> 

<223> muTNFa peptide 
<400> 181 

Cys Gly Gly Val Glu Glu Gin Leu Glu Trp Leu Ser Gin Arg 
15 10 

<210> 182 

<211> 22 

<212> PRT 

<213> Artificial Sequence 
<220> 

<223> TNFa II ( 3 ' -TNFa II) 
<40O> 182 

Ser Ser Gin Asn Ser Ser Asp Lys Pro Val Ala His Val Val Ala Asn 
15 10 15 

His Gly Val Gly Gly Cys 
20 

<210> 183 
<211> 20 



<212> PRT 

<213> Artificial Sequence 



<220> 

<223> TNFa II (5' TNFa II) 



<400> 183 

Cys Ser Ser Gin Asn Ser Ser Asp Lys Pro Val Ala His Val Val Ala 
1 5 10 15 

Asn His Gly Val 
20 

<210> 184 
<211> 182 
<212> PRT 

<213> Escherichia coli 
<400> 184 

Met Lys lie Lys Thr Leu Ala He Val Val Leu Ser Ala Leu Ser Leu 
15 10 15 

Ser Ser Thr Ala Ala Leu Ala Ala Ala Thr Thr Val Asn Gly Gly Thr 
20 25 30 

Val His Phe Lys Gly Glu Val Val Asn Ala Ala Cys Ala Val Asp Ala 
35 40 45 

Gly Ser Val Asp Gin Thr Val Gin Leu Gly Gin Val Arg Thr Ala Ser 
50 55 60 

Leu Ala Gin Glu Gly Ala Thr Ser Ser Ala Val Gly Phe Asn He Gin 
65 70 75 80 

Leu Asn Asp Cys Asp Thr Asn Val Ala Ser Lys Ala Ala Val Ala Phe 
85 90 95 

Leu Gly Thr Ala He Asp Ala Gly His Thr Asn Val Leu Ala Leu Gin 
100 105 HO 

Ser Ser Ala Ala Gly Ser Ala Thr Asn Val Gly Val Gin He Leu Asp 
115 120 125 

Arg Thr Gly Ala Ala Leu Thr Leu Asp Gly Ala Thr Phe Ser Ser Glu 
130 135 140 

Thr Thr Leu Asn Asn Gly Thr Asn Thr He Pro Phe Gin Ala Arg Tyr 
145 150 155 160 



-99- 

Phe Ala Thr Gly Ala Ala Thr Pro Gly Ala Ala Asn Ala Asp Ala Thr 
165 170 175 

Phe Lys Val Gin Tyr Gin 
180 

<210> 185 
<211> 152 
<212> PRT 

<213> Hepatitis B virus 



<400> 185 

Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 

Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
20 25 30 

Thr Ala Ser Ala Leu Tyr Arg Glu Ala He Glu Ser Pro Glu His Cys 
35 40 45 

Ser Pro His His Thr Ala Leu Arg Gin Ala He Leu Cys Trp Gly Glu 
50 55 60 

Leu Met Thr Leu Ala Thr Trp Val Gly Thr Asn Leu Glu Asp Gly Gly 
65 70 75 80 

Lys Gly Gly Ser Arg Asp Leu Val Val Ser Tyr Val Asn Thr Asn Met 
85 90 95 

Gly Leu Lys He Arg Gin Leu Leu Trp Phe His He Ser Cys Leu Thr 
100 105 HO 

Phe Gly Arg Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp 
115 120 125 

He Arg Thr Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro He Leu Ser 
130 135 140 

Thr Leu Pro Glu Thr Thr Val Val 
145 150 

<210> 186 

<211> 152 

<212> PRT 

<213> Hepatitis B virus 



<400> 186 



Met Asp He Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
15 10 15 



-100- 



Ser Phe Leu Pro Ser Asp Phe Phe 
20 



Thr Ala Ser Ala Leu Tyr Arg Glu 
35 40 

Ser Pro His His Thr Ala Leu Arg 
50 55 

Leu Met Thr Leu Ala Thr Trp Val 
65 70 



Lys Gly Gly Ser Arg Asp Leu Val 
85 



Gly Leu Lys lie Arg Gin Leu Leu 
100 



Phe Gly Arg Glu Thr Val Leu Glu 
115 120 



lie Arg Thr Pro Pro Ala Tyr Arg 
130 135 



Pro Ser Val Arg Asp Leu Leu Asp 
25 ■ 30 



Ala Leu Glu Ser Pro Glu His Ser 
45 



Gin Ala lie Leu Cys Trp Gly Glu 
60 



Gly Thr Asn Leu Glu Asp Gly Gly 
75 80 

Val Ser Tyr Val Asn Thr Asn Met 
90 95 

Trp Phe His lie Ser Ser Leu Thr 
105 110 



Tyr Leu Val Ser Phe Gly Val Trp 
125 



Pro Pro Asn Ala Pro lie Leu Ser 
140 



Thr Leu Pro Glu Thr Thr Val Val 
145 150