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Full text of "USPTO Patents Application 09919224"

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WHAT IS CLAIMED IS: 

1 1 . A method of inhibiting the proliferation of a peripheral blood 

2 mononuclear cell population, comprising contacting the peripheral blood mononuclear cell 

3 population with an amount of rhesus or human CMV IL-10 sufficient to inhibit the proliferation 

4 of the peripheral blood mononuclear cell population. 

1 2. The method of claim 1, wherein the peripheral blood mononuclear 

2 population is contacted with rhesus CMV IL-1 0. 

1 3 . The method of claim 1 , wherein the peripheral blood mononuclear 

2 population is contacted with human CMV IL-10. 

1 4. The method of claim 1, wherein peripheral blood mononuclear, cells are 

2 proliferating when the contacting step is performed. 

1 5. The method of claim 1, wherein the contacting occurs in vitro. 

1 6. The method of claim 1, further comprising adding an agent that induces 

2 the peripheral blood mononuclear cells to proliferate. 

1 7. The method of claim 1 , wherein the level of IFN-y secreted by the 

2 peripheral blood mononuclear is cells is detectably reduced responsive to the contacting step. 

1 8. The method of claim 1 , wherein the level of TNF-a secreted by the 

2 peripheral blood monocular cells is detectably reduced responsive to the contacting step. 

1 9. The method of claim 1 , further comprising monitoring the proliferation 

2 level of the peripheral blood mononuclear cells to determine a reduction in the proliferation level 

3 responsive to the contacting step. 

1 1 0. The method of claim 1 , further comprising monitoring secretion of IFN-y 

2 or TNF-a to determine a reduction in level of secreted IFN-y or TNF-a responsive to the 

3 contacting step. 



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1 11. The method of claim 1 , wherein the mononuclear proliferating cells 

2 are rhesus or human cells. 

1 12. A method of reducing cytokine production of a monocyte cell population, 

2 comprising contacting the monocyte cell population with an amount of rhesus or human CMV 

3 IL-10 sufficient to reduce cytokine production by the monocyte cell population. 

1 13. The method of claim 12, wherein the contacting occurs in vitro. 

1 14. The method of claim 12, wherein the level of IFN-y secreted by the 

2 monocytes is detectably reduced responsive to the contacting step. 

1 15. The method of claim 1 2, wherein the level of TNF-a secreted by the 

2 monocytes is detectably reduced responsive to the contacting step. 

1 16. The method of claim 1 2, wherein the level of GM-CSF secreted by the 

2 monocytes is detectably reduced responsive to the contacting step. 

1 17. The method of claim 1 2, wherein the level of IL- 1 a secreted by the 

2 monocytes is detectably reduced responsive to the contacting step. 

1 18. The method of claim 12, wherein the level of IL-6 secreted by the 

2 monocytes is detectably reduced responsive to the contacting step. 

1 19 The method of claim 12, further comprising monitoring the cytokine 

2 levels of the monocytes to determine a reduction in the proliferation level responsive to the 

3 contacting step. 

1 20. The method of claim 1 2, further comprising monitoring secretion of IFN- 

2 y, TNF-a, GM-CSF, IL-la or IL-6 to determine a reduction in level of secreted IFN-y, TNF-a, 

3 GM-CSF, IL-la or IL-6, responsive to the contacting step. 

21. A method of preventing or treating an immune disorder in a patient, 

2 com^ising: 



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ru 



3 administering rhesus CMV IL-10 or human CMV IL-10 to a patient suffering 

4 from or susceptible td the disorder in a dosage sufficient to inhibit proliferation of 

5 lymphocytes in the parent, and thereby prevent or treat the disorder. 

1 22. The method of claim 21, wherein the rhesus CMV IL-10 or human CMV 

2 IL-10 is a component o\ a pharmaceutical composition further comprising a pharmaceutical^ 

3 acceptable carrier. 

1 23 . Tha method of claim 2 1 , wherein the pharmaceutical composition is 

2 sterile, substantially isotonic and prepared under GMP conditions. 

1 24. The method of claim 21, wherein the patient is suffering from or 

2 susceptible to an immune disorder selected from the group consisting of graft versus host 

3 disease, an autoimmune disease, an inflammatory response, a pathologic delayed type 

4 hypersensitivity response, enabtoxin-induced toxic shock, granulomatis disease, psoriasis, 

5 uveitis, systemic lupus erythematous, multiple sclerosis and contact-dermatitis. 



1 25. The methou of claim 2 1 , further comprising monitoring proliferation of 

* 2 the lymphocytes in the patient to c^etect a reduction in the level of proliferation responsive to the 

is 

□ 3 administering step. 

1 26. The method ofi claim 2 1 , further comprising monitoring a symptom of the 

| a f 2 patient, to detect amelioration or prevention of the symptom responsive to the administering 

3 step. 

1 27. The method of clain}\J2 1 , wherein the patient is suffering from the 

2 disorder. 

1 .28. The method of claim 21, wherein the patient is susceptible to the disorder. 

1 29. The method of claim 28, wherein the patient is an organ transplant patient. 

1 30. The method of claim 29, wherein the organ is a kidney. 



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1 a>«^Sf^L The Aiethod of claim 30, wherein the IFN-a levels are detectably 




2 decreased responsive to the administering of rhesus or human CMV IL-10. 



1 32. The method of claim 2 1 , wherein the inflammatory disorder is a chronic 

2 inflammatory response. 

1 33. The methok of claim 32 wherein the chronic inflammatory disease is 

2 selected from the group consistingW rheumatoid arthritis, inflammatory bowel disease, Crohn's 

3 disease, ulcerative colitis, Graves' oisease, Hashimoto's thyroiditis, systemic lupus 

4 erythematosus, multiple sclerosis, scleroderma, and insulin-dependent diabetes mellitus. 

1 34. The method of ojaim 2 1 , wherein the inflammatory disorder is an allergic 

2 response. 

'% 1 35. The method of claim 34, wherein the inflammatory disorder is asthma. 

=3 1 36. The method of claim \l , wherein the patient is suffering from a type T H 1 

rU 2 jgHB^ g ^ s P onse ' tQ tr ^M ^edgraft^ 

m 1 37. The method of claim 36, wherein the transplanted graft is an organ 

N 2 selected from the group consisting of cornea, lung, heart, liver, bone marrow, kidney, pancreas, 

:3 3 blood, and skin. 

^ \ 

^58. The method of claim 25 wherein the immune disorder is leukemia 

1 39. A method of ameliorating symptoms of hepatitis in an animal host, 

2 comprising administering to the animal infected with hepatitis virus an effective dosage CMV 

3 IL-10 sufficient to ameliorate at least one of the symptoms of hepatitis. 

1 40. The method of claim 39, wherein the administering step ameliorates 

2 damage liver in the patient. 

1 41 . The method of claim 39, wherein the administering step ameliorates liver 

2 disease or liver fibrosis. 



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if # 



1 42. A method of treating or preventing a respiratory viral infection in a 

2 patient, comprising administering rhesus or human CMV IL-10 to the patient suffering from or 

3 susceptible to a virally infected respiratory system in a dosage sufficient to ameliorate at least 

4 one symptom of the respiratory viral infection. 

1 43. A method for reducing an in vivo inflammatory response characterized by 



2 substantially elevated levels of at least one cytokine selected from the group consisting of IL-lct, 

3 GM-CSF, IFN-y and TNF-ct, comprising administering to the patient afflicted with such an 

4 inflammatory response or at risk for developing such an inflammatory response, an effective 

5 dosage of rhesus CMV IL-10 or human CMV IL-10 to substantially lower the levels of said 

6 cytokines. 



□ 1 ahc{\^ ^ method of preventing or treating the symptoms of an inflammatory 

5 2 respprree, comprising administering rhesus CMV IL-10 or human CMV IL-10 to the patient 

\ % 3 suffering from or susceptible to\an inflammatory response in a dosage sufficient to ameliorate at 

[U 4 least some of the symptoms of tne inflammatory condition. 

iH : 1 45 . The method of claim 44, further comprising monitoring proliferation of 

!;1 2 the lymphocytes in the patient to detect a reduction in the level of proliferation responsive to the 

w 3 administering step. \ 

1 46. The method of clainr44, further comprising monitoring a symptom of the 

2 patient, to detect amelioration or prevention of the symptom responsive to the administering 

3 step. \ 

1 47. The method of claim 44, wherein the patient is suffering from the 

2 disorder. \ 

1 48. The method of claim 44 wherein theunflammatory response is a chronic 

2 inflammatory response. 



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t 



1 49. The method of claim 48 wherein the chronic inflammatory disease is 

2 selected from the group consisting of rheumatoid arthritis, Crohn's disease, ulcerative colitis, 

3 Graves 1 disease, Hashimoto's thyroiditis and insulin-dependent diabetes mellitus. 




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