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Full text of "USPTO Patents Application 10617998"

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50704/KMO/B583 



Claims 

1. An amino acid sequence that is a mutant of an enzyme selected from the group 
consisting of leucine dehydrogenase sequences as described in SEQ ID 2, formate 
dehydrogenase sequence as described in SEQ ID 1, galactose oxidase sequences as described 
in SEQ ID 3, and substantial equivalents thereof, wherein: 

when the amino acid sequence is a mutant of a leucine dehydrogenase sequence as 
described in SEQ ID 2 or a substantial equivalent thereof, the amino acid sequence contains 
at least one mutation selected from the group consisting of F102S, V33A, S351T, N145S and 
like mutations in subsantially equivalent sequences; 

when the amino acid sequence is a mutant of a formate dehydrogenase sequence as 
described in SEQ ID I or a substantial equivalent thereof, the amino acid sequence contains 
at least one mutation selected from the group consisting of D195S, Y196H, K356T and like 
mutations in subsantially equivalent sequences; and 

when the amino acid sequence is a mutant of a galactose oxidase sequence as 
described in SEQ ID 3 or a substantial equivalent thereof, the amino acid sequence contains 
at least one mutation selected from the group consisting of N25Y, T94A, D216N, R217C, 
M278T, Y329C, Q406R, Q406L, V492A, V494A, N521S, N535D, T549I, S567T, T578S 
and like mutations in subsantially equivalent sequences. 

2. An amino acid sequence according to claim 1, wherein the sequence is a 
mutant of a leucine dehydrogenase sequence as described in SEQ ID 2 or its substantial 
equivalent. 

3. An amino acid sequence according to claim 1, wherein the mutant is a mutant 
of a leucine dehydrogenase sequence as described in SEQ ID 2. 

4. An amino acid sequence according to claim 1, wherein the mutant is a mutant 
of a leucine dehydrogenase sequence that is at least 45% homologous to the sequence 
described in SEQ ID 2. 



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1 50704/KMO/B583 

5. An amino acid sequence according to claim 1, wherein the mutant is a mutant 
of a leucine dehydrogenase sequence that is at least 70% homologous to the sequence 

5 described in SEQ ID 2. 

6. An amino acid sequence according to claim 1, wherein the mutant is a mutant 
of a leucine dehydrogenase sequence that is at least 80% homologous to the sequence 

j^Q described in SEQ ID 2. 

7. An amino acid sequence according to claim 1, wherein the mutant is a mutant 
of a leucine dehydrogenase sequence that is at least 95% homologous to the sequence 
described in SEQ ID 2. 

15 

8. A deoxyribonucleic acid molecule containing a DNA sequence encoding the 
amino acid sequence of claim 2. 

20 9. An amino acid sequence according to claim 1, wherein the sequence is a 

mutant of a formate dehydrogenase sequence as described in SEQ ID 1, or its substantial 
equivalent. 

2^ 10. An amino acid sequence according to claim 1, wherein the mutant is a mutant 

of a formate dehydrogenase sequence as described in SEQ ED 1. 

11. An amino acid sequence according to claim 1, wherein the mutant is a mutant 
of a formate dehydrogenase sequence that is at least 80% homologous to the sequence 

30 

described in SEQ ID 1. 

12. An amino acid sequence according to claim 1, wherein the mutant is a mutant 
of a formate dehydrogenase sequence that is at least 95% homologous to the sequence 

35 described in SEQ ID 1. 



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1 50704/KMO/B583 

13. A deoxyribonucleic acid molecule containing a DNA sequence encoding the 
amino acid sequence of claim 9. 

5 

14. An amino acid sequence according to claim 1, wlierein the sequence is a 
mutant of a galactose oxidase sequence as described in SEQ ID 3, or its substantial 
equivalent. 

10 

15. An amino acid sequence according to claim 1, wherein the mutant is a mutant 
of a galactose oxidase sequence as described in SEQ ID 3. 

16. An amino acid sequence according to claim 1, wherein the mutant is a mutant 

15 

of a galactose oxidase sequence that is at least 80% homologous to the sequence described in 
SEQ ID 3. 

17. An amino acid sequence according to claim 1, wherein the mutant is a mutant 
20 of a galactose oxidase sequence that is at least 95% homologous to the sequence described in 

SEQ ID 3. 

18. A deoxyribonucleic acid molecule containing a DNA sequence encoding the 
2^ amino acid sequence of claim 14. 

19. A method for the production of an amino acid that comprises contacting a 
ketoacid with the amino acid sequence of claim 2 in the presence of a reduced nicotinamide 
cofactor and an ammonia source. 

30 

20. A method for the recycling of a nicotinamide cofactor that comprises 
contacting an oxidized nicotinamid cofactor with an amino acid sequence of claim 9 in the 
presence of a formate source. 

35 



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